Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	117,253	T→C	6.3%	intergenic (+33/+61)	PP_0111 → / ← metQ	electron transport protein SCO1/SenC/methionine ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	117,253	0	T	C	6.3%	179.0 / 2.1	63	intergenic (+33/+61)	PP_0111/metQ	electron transport protein SCO1/SenC/methionine ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (30/29);  new base C (2/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

ACAGCGGTAACCTGGCGATTGTCGGCCCGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCTCAGGCGAGTGCAGTGCAGCCGCCAGTTTC  >  NC_002947/117105‑117398                                                                                                                                                     |                                                                                                                                                    acaGCGGTAACCTGGCGATTGTCGGCCCGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCt                                                                                                                                                     >  1:237462/1‑149 (MQ=255) acaGCGGTAACCTGGCGATTGTCGGCCCGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCt                                                                                                                                                     >  2:212697/1‑149 (MQ=255)  caGCGGTAACCTGGCGATTGTCGGCCCGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTc                                                                                                                                                    >  4:138932/1‑149 (MQ=255)    gCGGTAACCTGGCGATTGTCGGCCCGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTcc                                                                                                                                                   >  3:24668/1‑148 (MQ=255)      ggTAACCTGGCGATTGTCGGCCCGGATGGGAGACAGCGTGTGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAATCTACGCGGGTAAACCCGCTCCT‑a                                                                                                                                                <  6:203049/148‑1 (MQ=255)        tAACGTGGCGATTGGCGGCCCGGATGGGAGACAGCGGGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACaa                                                                                                                                             <  6:130707/149‑1 (MQ=255)          aCCTGGCGATTGTCGGCCCGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTc                                                                                                                                                    <  2:60445/141‑1 (MQ=255)          aCCTGGCGATTGTCGGCCCGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTc                                                                                                                                                    >  1:60445/1‑141 (MQ=255)            cTGGCGATTGTCGGCCCGGATGGGAGACAGCGTGGGTTCCTTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGAt                                                                                                                                         >  5:78286/1‑149 (MQ=255)               gCGATTGTCGGCCCGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGAtgt                                                                                                                                       <  7:66278/148‑1 (MQ=255)               gCGATTGTCGGCCCGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGGCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGAtgt                                                                                                                                       <  6:78286/148‑1 (MQ=255)                 gATTGTCGGCCCGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCCCCT‑ACacacatcg                                                                                                                                       <  1:132812/146‑8 (MQ=255)                 gATTGTCGGCCCGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCCCCT‑ACacacatcg                                                                                                                                       >  2:132812/1‑139 (MQ=255)                          cccGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCgg                                                                                                                          <  4:270833/149‑1 (MQ=255)                           gcGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCTTTCAACAACCTGAAGCTGTGGTCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCGCCAAAAGGTACGCTGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCggg                                                                                                                         <  3:101927/148‑1 (MQ=255)                           ccGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCggg                                                                                                                         >  6:302998/1‑149 (MQ=255)                            cGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCTAAAGCTTCGCGGGTAAACCCGCCCCT‑ACACACATCG‑TTGTAGGAGCGGGt                                                                                                                        <  1:243410/149‑1 (MQ=37)                            cGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCggg                                                                                                                         <  1:42627/148‑1 (MQ=255)                             ggATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGtt                                                                                                                       <  3:138932/149‑1 (MQ=255)                               aTGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGACCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGCGGCGGGTTTa                                                                                                                     <  5:82889/149‑1 (MQ=255)                                   gagaCAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCcg                                                                                                                 >  5:25563/1‑149 (MQ=255)                                    agaCAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGAt                                                                                                                                         <  7:300821/125‑1 (MQ=255)                                    agaCAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGAt                                                                                                                                         >  8:300821/1‑125 (MQ=255)                                        aGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCTCACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCgaa                                                                                                             <  1:212697/149‑1 (MQ=255)                                          cGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCgaagaa                                                                                                          <  4:301646/149‑1 (MQ=255)                                           gTGGTTTCATTCGGGCGCCGTTCAACAACCATAAGGTGGGGGCGCAGTTGCCGGGGCTGGTCATGGGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCAT‑ACAACTATGT‑GTGTTGGGGCGGGTTTACCCGCGAAGAAt                                                                                                         <  6:25563/149‑1 (MQ=255)                                           gTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCcgcg                                                                                                               <  8:175648/143‑1 (MQ=255)                                           gTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCcgcg                                                                                                               >  7:175648/1‑143 (MQ=255)                                           gTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCA‑ACAACGATGTAGTGTAGGGGCGGGTTTACCCGCgaagaa                                                                                                          <  1:185579/149‑1 (MQ=255)                                              ggTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATaga                                                                                                      <  3:300551/149‑1 (MQ=255)                                                           gcCGTTCAACAACCAGAAGCTGGGGGCGCAGTAGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCCCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAAAAGAGCGTGATAGGTCg                                                                                         >  4:131513/1‑149 (MQ=255)                                                                 caacaaCCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGAt                                                                                      >  6:74986/1‑146 (MQ=255)                                                                 caaaaaCCAGAAGCTGGGTGCGCAGTTGCCGGGGCTGTTCAAGCGGGATTTAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCa                                                                                    <  6:119168/148‑1 (MQ=255)                                                                  aacaacCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTTCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAg                                                                                   >  4:233669/1‑148 (MQ=255)                                                                   acaacCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGaa                                                                                 >  8:209503/1‑149 (MQ=255)                                                                   acaacCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCACAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGaa                                                                                 >  8:205720/1‑149 (MQ=255)                                                                        cAGAAGCTGGGGGCGCAGTTGCGGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCg                                                                            <  1:123704/149‑1 (MQ=255)                                                                          gAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑CCAACGCTGT‑GTGTAGGGGCGCGGTAACCCGCGCAGAATAGAGCTAGATAGGGCGGCTCAGACCGCCgg                                                                           >  2:236156/1‑148 (MQ=255)                                                                          gAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATa                                                                                                        <  5:233081/119‑1 (MQ=255)                                                                          gAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATa                                                                                                        >  6:233081/1‑119 (MQ=255)                                                                            aGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACTTTGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAAc                                                                        <  4:24668/149‑1 (MQ=255)                                                                              cTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTAc                                                                                                                    >  5:323899/1‑103 (MQ=255)                                                                              cTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTAc                                                                                                                    <  6:323899/103‑1 (MQ=255)                                                                                gggggCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACca                                                                      >  2:229293/1‑147 (MQ=255)                                                                                       cAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCtt                                                             <  1:233039/149‑1 (MQ=255)                                                                                        aGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGAGAAGAATATAGCTTGATAGGTCGGATCAGAACGCCGGACCCACAGCGCCttt                                                            >  8:298255/1‑149 (MQ=255)                                                                                         gTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTGGATAGGTCGGATCAGCACGCCGGAACCACAGCGCCATTg                                                           >  2:285948/1‑149 (MQ=255)                                                                                                 ggCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTtctc                                                   >  3:80293/1‑149 (MQ=255)                                                                                                 ggCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTtctc                                                   <  5:74986/149‑1 (MQ=255)                                                                                                        cAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCgatgat                                             >  7:54123/1‑148 (MQ=255)                                                                                                         aaGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGatgatg                                            <  2:192107/148‑1 (MQ=255)                                                                                                            agggATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAAAGATAT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTAGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACt                                        >  6:122371/2‑149 (MQ=255)                                                                                                                      tCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCt                               >  5:269523/1‑148 (MQ=255)                                                                                                                      tCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCt                               >  1:142267/1‑148 (MQ=255)                                                                                                                        aGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCTCAg                            <  6:269523/149‑1 (MQ=255)                                                                                                                          ccccAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCTCAGGc                          >  3:254288/1‑149 (MQ=255)                                                                                                                            ccAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCTCAGGc                          >  3:292349/1‑147 (MQ=255)                                                                                                                             cAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCTCAGGCGag                       >  1:301278/1‑149 (MQ=255)                                                                                                                                  gCTACGGGGGAAAACCCGCTCCT‑CCAACTTGGG‑GGTTCGGGGGGGTTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCTCAGGCGagtgcag                  <  2:142267/149‑1 (MQ=255)                                                                                                                                      cgcgGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCTCAGGCGagtgcagtg                >  5:270693/1‑147 (MQ=255)                                                                                                                                           gTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTACTCGATGATGAACTGCTTCACCTCAGGCGAGTGCAGTGCAgccgc         <  1:229293/149‑1 (MQ=255)                                                                                                                                            tAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCTCAGGCGAGTGCAGTGCAgccgc         >  5:53161/1‑148 (MQ=255)                                                                                                                                              aaCCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCTCAGGCGAGTGCAGTGCAGCCGCCAg      >  3:131779/1‑149 (MQ=255)                                                                                                                                                 ccGCCCCT‑ACAAACATCG‑TTGTAGGAGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAAAGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCTCAGGCGAGTGCAGTGCAGCCGCCAGttt   >  4:180940/1‑149 (MQ=38)                                                                                                                                                  cGCTGCT‑ACACCTTTGT‑GTGTAGGGGCGGTTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCTCAGGCGAGTGCAGTGCAGCCGCCAGTttc  <  8:114375/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    ACAGCGGTAACCTGGCGATTGTCGGCCCGGATGGGAGACAGCGTGGGTTCATTCGGGCGCCGTTCAACAACCAGAAGCTGGGGGCGCAGTTGCCGGGGCTGGTCAAGCGGGATTGAGTCAGCCCCAAAAGCTACGCGGGTAAACCCGCTCCT‑ACAACGATGT‑GTGTAGGGGCGGGTTTACCCGCGAAGAATAGAGCTTGATAGGTCGGATCAGAACGCCGGAACCACAGCGCCTTTGTACTTCTCGATGATGAACTGCTTCACCTCAGGCGAGTGCAGTGCAGCCGCCAGTTTC  >  NC_002947/117105‑117398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑