Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 782,064 A→T 9.4% intergenic (‑219/+31) glyA‑II ← / ← PP_0672 serine hydroxymethyltransferase/sensory box protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947782,0640AT 9.4% 150.9 / 4.9 53intergenic (‑219/+31)glyA‑II/PP_0672serine hydroxymethyltransferase/sensory box protein
Reads supporting (aligned to +/- strand):  ref base A (26/22);  new base T (3/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GTCAAGTCGCAGACCAATGGGCTTCATCTTGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGATAGCCCTGCACCTCGTCGCAACCATGCTCGCGCAGGAACTC  >  NC_002947/781920‑782211
                                                                                                                                                |                                                                                                                                                   
gTCAAGTCGCAGACCAATGGGCTTCATCTTGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTGCGCGGGTTTAccc                                                                                                                                                 >  2:206673/1‑149 (MQ=255)
     gTCGCAGTCCTATGGGCTTGATGTTGCTTCCCGCGCGGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGATTGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCgcgc                                                                                                                                             <  3:66167/148‑1 (MQ=38)
      tCGCAGACCAATGGGCTTCATCTTGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTGCGCGGGTTTACCcgcgaac                                                                                                                                           <  1:206673/149‑4 (MQ=255)
      tCGCAGACCAATGGGCTTCATCTTGCATACCGCGCCGTTTCAGGGCTGGGCGGGGTCGGGAGTTGAACTTGAAGTCAGCTTTTTTATGCAGTAATGCGGCGCGGTGGATGGCACCTGCTTTGCCGGTGTTCGCGGGTAAACCCGCGcc                                                                                                                                            >  4:118722/1‑148 (MQ=38)
       cGCAGACCAATGGGCTTCATCTTGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAAc                                                                                                                                                   >  4:209422/1‑140 (MQ=255)
       cGCAGACCAATGGGCTTCATCTTGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAAc                                                                                                                                                   <  3:209422/140‑1 (MQ=255)
        gCAGACCAATGGGCTTCATCTTGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTAc                                                                                                                                         <  2:159645/149‑1 (MQ=255)
              cAATGGGCTTCATCTTGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTTTACCcgcgaa                                                                                                                                            <  6:285196/140‑3 (MQ=255)
              cAATGGGCTTCATCTTGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTTTACCcgcgaa                                                                                                                                            >  5:285196/1‑138 (MQ=255)
              cAATGGGCTTCATCTTGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGc                                                                                                                                   <  4:159796/149‑1 (MQ=255)
              cAATGGGCTTCATCTTGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGAAGGAATGCGGCGCGGTGGTTGGCACCGGATTTGCCGGTGTTCGCGGGTAAACCCCCGCATACGGGGGc                                                                                                                                   >  6:46758/1‑149 (MQ=39)
                    gcatCATGTTGCATCCCGGGCCGTTTCTGGGCGGGGCGGGGGCGGGATGTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGTTGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTtgtg                                                                                                                             <  5:46758/146‑1 (MQ=38)
                           cTTGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCtt                                                                                                                                <  7:199142/139‑1 (MQ=255)
                           cTTGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCtt                                                                                                                                >  8:199142/1‑139 (MQ=255)
                             tGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTc                                                                                                                     >  4:261580/1‑148 (MQ=255)
                               cATCCGGCGCCGTTTCTGGTGTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCaa                                                                                                                   <  6:218922/148‑1 (MQ=255)
                                aTCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATg                                                                                                                 <  7:3361/149‑1 (MQ=255)
                                aTCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATg                                                                                                                 <  3:257188/149‑1 (MQ=255)
                                   ccGCGCCGTTTCTGGGCTGGGCTGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGAGGGCACCGGCTTTGCCGGTGTTCGGTGGTAACCCCGCGCCTACGGGGGCCTTGGGTGCGGGTCAATGGaa                                                                                                              <  1:230928/149‑1 (MQ=255)
                                     gcgcCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACa                                                                                                            <  2:281313/149‑1 (MQ=255)
                                     gcgcCGTTTCGGGGCTGGGCGGGGGCGGTAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACa                                                                                                            <  2:312729/149‑1 (MQ=255)
                                       gcCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACAt                                                                                                           >  8:25142/1‑148 (MQ=255)
                                           tttCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAAc                                                                                                       >  3:137483/1‑148 (MQ=255)
                                                  gctgggcGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGtctc                                                                                               >  4:153753/1‑149 (MQ=255)
                                                     gggcgggggaggGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCTGCGCGGTGGATTGCACCGGATTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCGTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGtt                                                                                            <  3:118722/149‑1 (MQ=39)
                                                         gggggcgggAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTg                                                                                           >  4:156263/1‑146 (MQ=255)
                                                          ggggcgggAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGTGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGgag                                                                                        >  6:6817/1‑148 (MQ=255)
                                                                 gAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTgcgc                                                                                <  7:63364/149‑1 (MQ=255)
                                                                                tCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATgcgc                                                                 >  2:69910/1‑149 (MQ=255)
                                                                                 ccGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCt                                                                >  8:182621/1‑149 (MQ=255)
                                                                                  cGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTg                                                               >  1:55249/1‑149 (MQ=255)
                                                                                    cTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAg                                                                                                    >  5:147420/1‑110 (MQ=255)
                                                                                    cTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAg                                                                                                    <  6:147420/110‑1 (MQ=255)
                                                                                         ttATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCgg                                                        >  1:216848/1‑149 (MQ=255)
                                                                                             gCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGgccgc                                                    >  6:194336/1‑149 (MQ=255)
                                                                                              cAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGgccgcc                                                   >  3:153559/1‑149 (MQ=255)
                                                                                                 gAATGCGGCGCGGTGGATGGCACAGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAAAATGAACAAGGTATCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCg                                                 >  2:31587/1‑148 (MQ=255)
                                                                                                   aTGCGGCGCGGTGGATGGCACCGGCGTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAAc                                              <  4:243208/149‑1 (MQ=255)
                                                                                                       tgcgcGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAg                                            <  3:26086/146‑1 (MQ=255)
                                                                                                       ggcgCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGa                                           >  6:147046/1‑148 (MQ=255)
                                                                                                         cgcgGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGGGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCGCGAACTGATGCGCTGGCAGCGGCCGCCCGACCAg                                            >  4:26086/1‑145 (MQ=255)
                                                                                                           cgGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGATAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGATAGc                                       <  5:6817/148‑1 (MQ=255)
                                                                                                                gATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGATAGCCCTGc                                  <  2:271705/148‑1 (MQ=255)
                                                                                                                  tGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCGTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGACCTGATGCGCTGGCATCGGCCGCCCGAACAGATAGCCCTGCAcc                               >  7:222965/1‑149 (MQ=255)
                                                                                                                     cACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGATAGCCCTGCACCtcg                            >  4:185990/1‑149 (MQ=255)
                                                                                                                                 cGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGATAGCCCTGCACCTCGTCGCAACCATGc                >  8:165300/1‑149 (MQ=255)
                                                                                                                                   gtgtTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGATAGCCCTGCACCTCGTCGCAACCATGCTc              >  6:86279/1‑149 (MQ=255)
                                                                                                                                       gcgcgGGTTTACCCGCGCCTACGGGGGCCGTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGATAGCCCTGCACCTCGTCGCAACCATGCTcgcg           >  1:205670/2‑148 (MQ=255)
                                                                                                                                        cgcgGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGATAGCCCTGCACCTCGTCGCAACCATGCTCgcgc          <  5:147046/148‑1 (MQ=255)
                                                                                                                                         gcgGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCTGAACAGATAGCCCTGCACCTCGTCGCAACCATGCTCGCGCa         <  4:153559/148‑1 (MQ=255)
                                                                                                                                         gcgGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGATAGCCCTGCACCTCGTCGCAACCATGCTCGCGCAg        >  5:84083/1‑149 (MQ=255)
                                                                                                                                              tAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGATAGCCCTGCACCtcgtc                          <  7:45485/126‑1 (MQ=255)
                                                                                                                                              tAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGATAGCCCTGCACCtcgtc                          >  8:45485/1‑126 (MQ=255)
                                                                                                                                               aaaCCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGATAGCCCTGCACCTCGTCGCAACCATGCTCGCGCAGGAACTc  >  4:272632/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GTCAAGTCGCAGACCAATGGGCTTCATCTTGCATCCCGCGCCGTTTCTGGGCTGGGCGGGGGCGGGAGTTGACCTGGAAGTCCGCTTTTTTATGCAGGAATGCGGCGCGGTGGATGGCACCGGCTTTGCCGGTGTTCGCGGGTAAACCCGCGCCTACGGGGGCCTTGTGTGCGGGTCAATGGAACATGAACAAGGTCTCGTTGGAGAATTGCGCCTCGAACTGATGCGCTGGCATCGGCCGCCCGAACAGATAGCCCTGCACCTCGTCGCAACCATGCTCGCGCAGGAACTC  >  NC_002947/781920‑782211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑