Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 787,188 C→G 7.9% A102A (GCC→GCG)  ettA → ADP/ATP ratio sensor/translation inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947787,1880CG 7.9% 144.7 / 4.4 51A102A (GCC→GCG) ettAADP/ATP ratio sensor/translation inhibitor
Reads supporting (aligned to +/- strand):  ref base C (25/21);  new base G (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-01

GAATTCGACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGCCC  >  NC_002947/787054‑787333
                                                                                                                                      |                                                                                                                                                  
gAATTCGACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCGGGCCTGGGCgtcct                                                                                                                                      <  1:99130/149‑5 (MQ=255)
gAATTCGACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCGGGCCTGGGCgtcct                                                                                                                                      <  6:159562/149‑5 (MQ=255)
  aTTCGACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTc                                                                                                                                     >  7:64051/1‑148 (MQ=255)
     cGACGGCGAAGCCCGTCCCATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAAACAAGACCGAGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTAcg                                                                                                                                 >  4:121463/1‑149 (MQ=255)
      gACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTAcgc                                                                                                                                >  5:247071/1‑149 (MQ=255)
         ggCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGc                                                                                                                             >  7:15687/1‑149 (MQ=255)
                               aCATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGAc                                                                                                       <  7:127242/149‑1 (MQ=255)
                                cATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACt                                                                                                      >  5:243052/1‑149 (MQ=255)
                                 aTCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACtt                                                                                                     <  8:15687/149‑1 (MQ=255)
                                    aaCGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGa                                                                                                  >  5:151333/1‑149 (MQ=255)
                                            ttACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGcc                                                                                                                 <  5:39803/126‑1 (MQ=255)
                                            ttACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGcc                                                                                                                 >  6:39803/1‑126 (MQ=255)
                                                cTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGgccgc                                                                                      >  1:224346/1‑149 (MQ=255)
                                                   ccACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGgccgcc                                                                                     >  3:7502/1‑147 (MQ=255)
                                                   ccACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGccgccg                                                                                    >  3:227784/1‑148 (MQ=255)
                                                   ccACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGccgccg                                                                                    <  4:227784/148‑1 (MQ=255)
                                                     acaGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCACGCCTGGACGCAGTCTACGCGGACTACGCCGAGCCGGATGCCGCCTGCGACAAGCAGGCCGCAGAGc                                                                                 >  4:219996/1‑149 (MQ=255)
                                                       aGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAg                                                                               <  6:247071/149‑1 (MQ=255)
                                                       aGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAg                                                                               >  2:61849/1‑149 (MQ=255)
                                                          aaCCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGcc                                                                            <  6:243052/149‑1 (MQ=255)
                                                               cAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCt                                                                       <  4:301505/149‑1 (MQ=255)
                                                                     gACCCTACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGGCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAg                                                                  <  1:290360/148‑1 (MQ=255)
                                                                     gACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGc                                                                 >  7:133145/1‑149 (MQ=255)
                                                                         caaccaaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑Tc                                                            <  4:7502/149‑1 (MQ=255)
                                                                         caaccaaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑Tc                                                            <  5:325998/149‑1 (MQ=255)
                                                                          aaccaaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGCAGCCA‑Tcc                                                           >  2:249121/1‑149 (MQ=255)
                                                                              aaGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTg                                                         >  1:289429/1‑147 (MQ=255)
                                                                               aGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAg                                                      <  8:267351/149‑1 (MQ=255)
                                                                                 aCCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGgc                                                    >  7:290890/1‑149 (MQ=255)
                                                                                     tgcgtgAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGgccgcc                                                >  6:280803/1‑149 (MQ=255)
                                                                                         ggAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATg                                            <  8:206666/148‑1 (MQ=255)
                                                                                          gAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATACCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATgg                                           >  6:273797/1‑149 (MQ=255)
                                                                                                   gAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCt                                  >  5:224967/1‑149 (MQ=255)
                                                                                                    aGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTg                                 >  8:93277/1‑149 (MQ=255)
                                                                                                    aGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTg                                 <  6:151333/149‑1 (MQ=255)
                                                                                                          cGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAgcgc                           <  8:290890/149‑1 (MQ=255)
                                                                                                            gTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAg                                                      <  8:265047/120‑1 (MQ=255)
                                                                                                            gTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAg                                                      >  7:265047/1‑120 (MQ=255)
                                                                                                             tCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAg                        >  2:213253/1‑149 (MQ=255)
                                                                                                                   tAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCATTCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGa                   <  1:61849/149‑1 (MQ=255)
                                                                                                                      tCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTc               <  5:105365/149‑1 (MQ=255)
                                                                                                                        aaGGACGCCCAGGCCCGCCTGGACGAGGCCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGc             >  2:274405/1‑149 (MQ=255)
                                                                                                                         acctcgtccAGGCGGGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCt            >  4:131770/8‑149 (MQ=255)
                                                                                                                         aGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCt            >  4:175851/1‑149 (MQ=255)
                                                                                                                           gACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGc          <  7:93277/149‑1 (MQ=255)
                                                                                                                           gACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGc          >  5:152725/1‑149 (MQ=255)
                                                                                                                            tcgtccAGGCGGGCCTGGGCGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGcc         >  7:158677/5‑149 (MQ=255)
                                                                                                                               cccAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATACCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGAc      <  5:273797/149‑1 (MQ=255)
                                                                                                                                ccAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACg     <  8:133145/149‑1 (MQ=255)
                                                                                                                                ccAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCCACg     >  4:239094/1‑149 (MQ=255)
                                                                                                                                   ggCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGccc  <  2:289429/149‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                                  
GAATTCGACGGCGAAGCCCGTCCGATGCCCGACATCAACGTGGGTTACCTGCCACAGGAACCGCAACTGGACCCAACCAAGACCGTGCGTGAAGTGGTCGAGGAAGCGGTCAGCGTAATCAAGGACGCCCAGGCCCGCCTGGACGAGGTCTACGCGGCCTACGCCGAGCCGGATGCCGACTTCGACAAGCTGGCCGCCGAGCAGGCCAAGCTGGAAGCCA‑TCCTGCAGGCCGCCGATGGCCACAACCTGGAGCGCCAGCTGGACGTCGCTGCCGACGCCC  >  NC_002947/787054‑787333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑