Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	788,584	A→T	7.9%	intergenic (+34/‑179)	ettA → / → gdhA	ADP/ATP ratio sensor/translation inhibitor/NADP‑dependent glutamate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	788,584	0	A	T	7.9%	183.1 / 4.7	63	intergenic (+34/‑179)	ettA/gdhA	ADP/ATP ratio sensor/translation inhibitor/NADP‑dependent glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (33/25);  new base T (3/2);  total (36/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

GTGTTCTTCGAAGGTAACTACACCGAGTACGAAGCCGACCGCAAGAAGCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTGGTACATTCCTGAA  >  NC_002947/788443‑788732                                                                                                                                              |                                                                                                                                                     gtgtTCTTCGAAGGTAACTACACCGAGTACGAAGCCGCCCGCAAGAAGCGTCTGGGTGCTGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACGGGCCCAGTAAGCCGGTTATCTCAGGCACAAGAATGGGGCCCCAAGTGcccc                                                                                                                                               >  2:266133/1‑149 (MQ=255) gtgtTCTTCGAAGGTAACTACACCGAGTACGAAGCCGACCGCAAGAAGCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGccc                                                                                                                                               >  6:190248/1‑149 (MQ=255)       ttcGAAGGTAACTACACCGAGTACGAAGCCGACCGCAAGAAGCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCAtttt                                                                                                                                         >  6:319893/1‑149 (MQ=255)         cGAAGGTAACTACACCGAGTACGAAGCCGACCGCAAGAAGCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTtg                                                                                                                                       >  7:128802/1‑149 (MQ=255)          gacggTAACTACACCTAGTCCGCCGCCGCCCGCATGAAGCGGCGGGGTGATGCCGCGGCCCTGCCGCTCCGGGTACGGCACAAGAAACTGGCCCAGTCTGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTtgt                                                                                                                                      <  1:266133/146‑1 (MQ=255)          gAAGGTAACTACACCGAGTACGAAGCCGACCGCAAGAAGCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTtgt                                                                                                                                      <  8:129858/149‑1 (MQ=255)              gTAACTACACCGAGTACGAAGCCGACCGCAAGAAGCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCt                                                                                                                                  <  4:10466/149‑1 (MQ=255)                     cacCGAGTACGAAGCCGACCGCAAGAAGCGTCTGGGTGATGCTGCTGGCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGTTGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGa                                                                                                                            <  4:311851/148‑1 (MQ=255)                     cacCGAGTACGAAGCCGACCGCAAGAAGCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAAAATGGGGCCACTTGGGGCCCCATTCTtgt                                                                                                                                      >  1:147734/1‑138 (MQ=38)                     cacCGAGTACGAAGCCGACCGCAAGAAGCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAAAATGGGGCCACTTGGGGCCCCATTCTtgt                                                                                                                                      <  2:147734/138‑1 (MQ=38)                           gTACGAAGCCGACCGCAAGAAGCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCtat                                                                                                                     <  1:33934/149‑1 (MQ=255)                               gatgCCGACGGCATGATGCGTCTGGGTTATGCTGCTGCCCAGCCGCAACGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATTCACAAGAATGGGGCCCCAAGTGGCTCAATTTTTGTGCCTGGCGGACCGCTATaaa                                                                                                                  <  2:203501/145‑1 (MQ=255)                               gAAGCCGAACGCAAGAAGCGTGTGGGTGATGCTTCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAAt                                                                                                                 <  7:275804/149‑1 (MQ=255)                                 aGCCGACCGCAAGAAGCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATgg                                                                                                               >  7:136267/1‑149 (MQ=255)                                     gACCGCAAGAAGCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATggcgag                                                                                                           <  6:151568/149‑1 (MQ=255)                                      aCCGCAAGAAGCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATggcgagg                                                                                                          <  3:56099/149‑1 (MQ=255)                                           aagaagCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAg                                                                                                     >  5:35182/1‑149 (MQ=255)                                           aagaagCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAg                                                                                                     <  8:59840/149‑1 (MQ=255)                                             gaagCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGgt                                                                                                   <  6:35182/149‑1 (MQ=255)                                                       ggTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGt                                                                                          <  2:308227/148‑1 (MQ=255)                                                            tgctgctgcCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATacac                                                                                    <  5:190248/149‑1 (MQ=255)                                                             gctgctgcCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACt                                                                                   >  5:20859/1‑149 (MQ=255)                                                               tgctgcCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTa                                                                                 >  4:151411/1‑149 (MQ=255)                                                               tgctgcCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTa                                                                                 >  1:259727/1‑149 (MQ=255)                                                                    cccAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTAGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATa                                                                             >  6:221203/1‑148 (MQ=255)                                                                     ccAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATaa                                                                            >  1:281229/1‑148 (MQ=255)                                                                     ccAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATaa                                                                            >  4:24823/1‑148 (MQ=255)                                                                      cAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATaaaa                                                                          >  3:268841/1‑149 (MQ=255)                                                                                 tgtACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAaat                                                                >  5:185560/1‑148 (MQ=255)                                                                                    aCGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAAtt                                                            >  8:226132/1‑149 (MQ=255)                                                                                         acaAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCaa                                                        >  7:82733/1‑148 (MQ=255)                                                                                             gAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCaaaaaaa                                                   <  8:128802/149‑1 (MQ=255)                                                                                                  tGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCAcc                                                                    <  6:260728/127‑1 (MQ=255)                                                                                                  tGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCAcc                                                                    >  5:260728/1‑127 (MQ=255)                                                                                                     cccAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACAtt                                            >  1:299213/1‑148 (MQ=255)                                                                                                           aaGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGccc                                      <  4:76456/148‑1 (MQ=255)                                                                                                             gCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTAt                                   >  7:176930/1‑149 (MQ=255)                                                                                                                    ttCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTACGCCCTATTCCCGTg                            >  8:304616/1‑149 (MQ=255)                                                                                                                       tGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTa                                                                                 <  3:238451/93‑1 (MQ=255)                                                                                                                       tGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTa                                                                                 >  4:238451/1‑93 (MQ=255)                                                                                                                         aTGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGAtt                       >  4:64971/1‑149 (MQ=255)                                                                                                                         aTGCACAAGAATGGGGCCCCAAGTGGCACCATTTTTGTTCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAATAAACGACATTTCGCCCTATTCCGGTGCGAt                        <  1:26301/148‑1 (MQ=255)                                                                                                                          tGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGAtt                       <  2:292155/148‑1 (MQ=255)                                                                                                                          tGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTg                      >  7:248101/1‑149 (MQ=255)                                                                                                                           gCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAGACGCACCAAATAATTTCAAAAAAACGAAATTTCGCCCTATTCCGGTGCGATTga                     >  4:43002/1‑148 (MQ=255)                                                                                                                           gCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGc                     >  6:286326/1‑149 (MQ=255)                                                                                                                             acaAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGctt                   <  2:281229/149‑1 (MQ=255)                                                                                                                             acaAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGctt                   >  5:141741/1‑149 (MQ=255)                                                                                                                              caAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGcttc                  <  2:299213/149‑1 (MQ=255)                                                                                                                               aaaaaTGGGGCCACTTGGGGCCCCATTCTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGcttct                 >  4:148920/4‑149 (MQ=255)                                                                                                                               aaGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGcttct                 >  4:11933/1‑149 (MQ=255)                                                                                                                               aaGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGcttct                 >  5:318867/1‑149 (MQ=255)                                                                                                                                 gAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTg               <  3:24823/149‑1 (MQ=255)                                                                                                                                 gAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTg               >  5:51286/1‑149 (MQ=255)                                                                                                                                 gAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTg               <  5:319893/149‑1 (MQ=255)                                                                                                                                 gAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGCATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTg               <  6:329922/149‑1 (MQ=255)                                                                                                                                 aaaTGGGGCCACTTGGGGCCCCATTCTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTg               <  3:148920/148‑1 (MQ=255)                                                                                                                                   aTGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTGGt             <  6:20859/149‑1 (MQ=255)                                                                                                                                    tGGGGCCACTTGGGGCCCCATTCTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTGGTa            >  1:125169/1‑149 (MQ=255)                                                                                                                                      gggCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTGGTACa          >  2:55104/1‑149 (MQ=255)                                                                                                                                      gggCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTGGTACa          >  4:261348/1‑149 (MQ=255)                                                                                                                                        gCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTGGTACAtt        >  3:29111/1‑149 (MQ=255)                                                                                                                                           ccAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTGGTACATTCCt     >  7:214382/1‑149 (MQ=255)                                                                                                                                              aGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTGGTACATTCCTGaa  <  8:136267/149‑1 (MQ=255)                                                                                                                                              aGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTGGTACATTCCTGaa  <  8:82733/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                     GTGTTCTTCGAAGGTAACTACACCGAGTACGAAGCCGACCGCAAGAAGCGTCTGGGTGATGCTGCTGCCCAGCCGCACCGTGTACGGCACAAGAAACTGGCCCAGTAAGCCGGTTTTCTGATGCACAAGAATGGGGCCCCAAGTGGCCCCATTTTTGTGCCTGGCGGACCGCTATAAATGGCGAGGCGAAGGTGTATTTTGTATACACTTACATAAAACGCACCAAATAATTTCAAAAAAACGACATTTCGCCCTATTCCGGTGCGATTGCTTCTTGGTACATTCCTGAA  >  NC_002947/788443‑788732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑