Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 828,921 C→G 6.4% T178S (ACT→AGT)  kefB‑I → CPA2 family Kef‑type potassium/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947828,9210CG 6.4% 200.8 / 4.2 62T178S (ACT→AGT) kefB‑ICPA2 family Kef‑type potassium/proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (30/28);  new base G (2/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

ACTGCCTTTGGCTTGCAGGTACTGGCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGCTGGTC  >  NC_002947/828776‑829069
                                                                                                                                                 |                                                                                                                                                    
aCTGCCTATGGCTTGCAGGTACTGGCCGAGCGCAAGGACCTGGGCAAACCTCATCGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGAGGAGCCGCTGCTCGGTGGCGATGTCAGCActcc                                                                                                                                                   >  7:103530/1‑147 (MQ=255)
        tGGCTTGCAGGTACTGGCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGaa                                                                                                                                            <  6:65148/148‑1 (MQ=255)
         ggCTTGCAGGTACTGGCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAg                                                                                                                                           <  1:211791/148‑1 (MQ=255)
          gCTTGCAGGTACTGGCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCAGTGCTGAcatcgc                                                                                                                                          <  3:153635/148‑6 (MQ=255)
                  gTACTGGCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACAGTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGcc                                                                                                                                 <  7:214077/149‑1 (MQ=255)
                   tACTGGCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGCCATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGCCGCTGGTGCCTGGccc                                                                                                                                >  1:113255/1‑149 (MQ=255)
                      tGGCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTg                                                                                                                             >  2:147965/1‑149 (MQ=255)
                        gCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGTCGAAGGTGCCTGGCCCTTgct                                                                                                                           <  7:161559/149‑1 (MQ=255)
                         ccGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTgctg                                                                                                                          <  1:221701/149‑1 (MQ=255)
                            aGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTgctggct                                                                                                                       >  7:177613/1‑149 (MQ=255)
                               gcAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCtgtg                                                                                                                    <  1:3510/149‑1 (MQ=255)
                               gcAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGATCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCtgtg                                                                                                                    <  5:121739/149‑1 (MQ=255)
                                      ccTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCg                                                                                                             <  1:22054/149‑1 (MQ=255)
                                       cTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTgctggctg                                                                                                                      >  1:300376/1‑139 (MQ=255)
                                       cTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTgctggctg                                                                                                                      <  2:300376/139‑1 (MQ=255)
                                         gggCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCa                                                                                                          >  6:134151/1‑149 (MQ=255)
                                               aCCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTg                                                                                                       <  4:236274/146‑1 (MQ=255)
                                               aCCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTg                                                                                                       >  3:236274/1‑146 (MQ=255)
                                                  tCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCAGTGCTGAcatc                                                                                                                                            <  5:46333/106‑4 (MQ=255)
                                                  tCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCAGTGCTGAcatc                                                                                                                                            >  6:46333/1‑103 (MQ=255)
                                                   catggccgcatggccgTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCt                                                                                                >  8:120443/1‑149 (MQ=255)
                                                     tggccgcatggccgTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGa                                                                                              <  1:147965/149‑1 (MQ=255)
                                                      ggccgcatggccgTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGAt                                                                                             >  4:315561/1‑149 (MQ=255)
                                                       gccgcatggccgTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGAtt                                                                                            >  3:120121/1‑149 (MQ=255)
                                                          gcatggccgTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGGGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCGGTGGTTGTCGGCATTGGTGTGCTGATTGtt                                                                                         <  6:109117/149‑1 (MQ=255)
                                                                                gTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCgg                                                                                                            >  6:204026/1‑108 (MQ=255)
                                                                                gTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCgg                                                                                                            <  5:204026/108‑1 (MQ=255)
                                                                                 ttCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTgg                                                                                    >  3:213143/1‑131 (MQ=255)
                                                                                 ttCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTgg                                                                                    <  4:213143/131‑1 (MQ=255)
                                                                                   ccAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCg                                                                 <  3:315561/148‑1 (MQ=255)
                                                                                    cAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGa                                                                >  8:46228/1‑148 (MQ=255)
                                                                                    cAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGa                                                                >  5:109511/1‑148 (MQ=255)
                                                                                     aGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGata                                                              >  3:78728/1‑149 (MQ=255)
                                                                                       gACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGatat                                                             >  8:157547/1‑148 (MQ=255)
                                                                                       gACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATAtt                                                            >  1:295623/1‑149 (MQ=255)
                                                                                          aTTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCTGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCaa                                                         <  1:194846/149‑1 (MQ=255)
                                                                                            ttccgccATGACCTTTATTGGCGTGGTGCCGCTGCTCGGGTGGGATTTGAGCACTTCTGTGGAATGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCaa                                                         <  8:103530/145‑1 (MQ=255)
                                                                                             gccgccATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGt                                                       <  7:157547/148‑1 (MQ=255)
                                                                                                gccATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGAc                                                   >  3:234120/1‑149 (MQ=255)
                                                                                                   aTTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTTCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGt                                                <  6:109511/149‑1 (MQ=255)
                                                                                                       ccTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGGACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTgg                                              <  8:116785/147‑1 (MQ=255)
                                                                                                       ccTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTgg                                              >  7:116785/1‑147 (MQ=255)
                                                                                                       ccTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGt                                            >  8:66289/1‑149 (MQ=255)
                                                                                                       ccTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGt                                            >  4:125981/1‑149 (MQ=255)
                                                                                                       ccTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGt                                            >  1:285761/1‑149 (MQ=255)
                                                                                                           gATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCgg                                        >  8:31058/1‑149 (MQ=255)
                                                                                                            atggCCGGGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCgg                                        <  8:239542/145‑1 (MQ=255)
                                                                                                               gCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGgcct                                    >  7:39299/1‑149 (MQ=255)
                                                                                                                ccGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGTTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGgcctg                                   >  3:170266/1‑149 (MQ=255)
                                                                                                                ccGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGgcctg                                   <  2:295623/149‑1 (MQ=255)
                                                                                                                 cGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGgcctgc                                  <  8:177613/149‑1 (MQ=255)
                                                                                                                   tggtgCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGgcctgcct                                >  4:244334/1‑149 (MQ=255)
                                                                                                                       gCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCACGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAAc                            >  5:45074/1‑149 (MQ=255)
                                                                                                                        ccGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACt                           >  3:220527/1‑149 (MQ=255)
                                                                                                                                        aTGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGcc           <  8:39299/149‑1 (MQ=255)
                                                                                                                                             aGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGCt      <  4:234120/149‑1 (MQ=255)
                                                                                                                                              gCAGTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGCTg     >  7:255993/1‑149 (MQ=255)
                                                                                                                                              gCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCGGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGCTg     >  6:70191/1‑149 (MQ=255)
                                                                                                                                              gCACTGCTGACGAAGGTGCCTGGACCTTGCTGGCTGTGGTTGTCGGAATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGCTg     >  7:201703/1‑149 (MQ=255)
                                                                                                                                              gCACTGCTGACGAAGGTGCCT‑GCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGcc           <  3:261199/142‑1 (MQ=255)
                                                                                                                                              gCACTGCTGACGAAGGTGCCT‑GCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGcc           >  4:261199/1‑142 (MQ=255)
                                                                                                                                               ccctgctgACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGCTgg    <  8:201703/147‑1 (MQ=255)
                                                                                                                                               cACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGCTgg    <  7:46228/149‑1 (MQ=255)
                                                                                                                                               cACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGCTgg    <  2:33531/149‑1 (MQ=255)
                                                                                                                                                 ctgctgACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGCTGGTc  <  2:285761/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ACTGCCTTTGGCTTGCAGGTACTGGCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGGCGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGGCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGCTGGTC  >  NC_002947/828776‑829069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑