Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 837,926 G→T 6.3% P13P (CCC→CCA)  pth ← peptidyl‑tRNA hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947837,9260GT 6.3% 235.2 / 6.0 79P13P (CCC→CCA) pthpeptidyl‑tRNA hydrolase
Reads supporting (aligned to +/- strand):  ref base G (34/40);  new base T (3/2);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-02

ACAAACGAACGTCGTTGCCCTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGA  >  NC_002947/837778‑838071
                                                                                                                                                    |                                                                                                                                                  
acaaACGAACGTCGTTGCCCTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCg                                                                                                                                                    >  5:75213/1‑149 (MQ=255)
 caaACGAACGTCGTTGCCCTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCgg                                                                                                                                                   >  8:186326/1‑149 (MQ=255)
  aaaCGAACGTCGTTGCCCTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCtgg                                                                                                                                                  >  1:34278/1‑149 (MQ=255)
  aaaCGAACGTCGTTGCCCTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCggg                                                                                                                                                  >  5:214208/1‑149 (MQ=255)
  aaaCGAACGTCGTTGCCCTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCggg                                                                                                                                                  >  2:22296/1‑149 (MQ=255)
  aaaCGAACGTCGTTGCCCTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCggg                                                                                                                                                  <  3:54674/149‑1 (MQ=255)
   aacgaacgTCGTTGCCCTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCggg                                                                                                                                                  >  1:125218/1‑148 (MQ=255)
   aacgaacgTCGTTGCCCTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCggg                                                                                                                                                  <  2:125218/148‑1 (MQ=255)
           tcgtTGCCCTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAg                                                                                                                                         <  7:6067/149‑1 (MQ=255)
             gtTGCCCTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAgg                                                                                                                                        <  1:22296/148‑1 (MQ=255)
              ttGCCCTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCTGGGTAACCCCGGcc                                                                                                                                      <  2:34278/149‑1 (MQ=255)
                 cccTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCaa                                                                                                                                    <  7:157883/148‑1 (MQ=255)
                 cccTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCaa                                                                                                                                    <  2:53609/148‑1 (MQ=255)
                  ccTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAAc                                                                                                                                   >  8:230351/1‑148 (MQ=255)
                  ccTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAAc                                                                                                                                   >  5:60351/1‑148 (MQ=255)
                  ccTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAAc                                                                                                                                   <  6:60351/148‑1 (MQ=255)
                    tGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGAt                                                                                                                                >  7:304245/1‑149 (MQ=255)
                           tGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGTTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTg                                                                                                                          >  8:62075/1‑148 (MQ=255)
                           tGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGGTACCCAGGCCAACGATCAACTgg                                                                                                                         >  8:66413/1‑149 (MQ=255)
                           tGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGCTCTGTTCGTATTCGGGGCCGTGGTTACCCAGGCCAACGATCAACTg                                                                                                                          >  7:156216/1‑148 (MQ=255)
                             aaTTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTgg                                                                                                                         <  7:275545/147‑1 (MQ=255)
                             aaTTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTgg                                                                                                                         <  1:63845/147‑1 (MQ=255)
                               tttAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATgg                                                                                                                     >  5:50369/1‑149 (MQ=255)
                                 tAGCCTGCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGc                                                                                                                    <  4:85548/148‑1 (MQ=255)
                                   gCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGt                                                                                                                 >  5:104582/1‑149 (MQ=255)
                                   gCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGt                                                                                                                 >  6:292558/1‑149 (MQ=255)
                                   gCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGt                                                                                                                 >  6:287690/1‑149 (MQ=255)
                                    ccGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGt                                                                                                                 >  8:150668/1‑148 (MQ=255)
                                       tCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACg                                                                                                             >  8:249323/1‑149 (MQ=255)
                                         aGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGt                                                                                                            <  1:107602/148‑1 (MQ=255)
                                           gCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAg                                                                                                         >  5:9092/1‑149 (MQ=255)
                                           gCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAg                                                                                                         <  6:9092/149‑1 (MQ=255)
                                            ccGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAgg                                                                                                        <  4:77888/149‑1 (MQ=255)
                                             cGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCg                                                                                                                   <  8:292392/137‑1 (MQ=255)
                                             cGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAggg                                                                                                       >  3:264979/1‑149 (MQ=255)
                                             cGAAAGACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCg                                                                                                                   >  7:292392/1‑137 (MQ=255)
                                                  tACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGctct                                                                                                  >  4:91796/1‑149 (MQ=255)
                                                    cTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTc                                                                                                >  7:295294/1‑149 (MQ=255)
                                                        ttGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGTGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCtt                                                                                             <  3:8401/148‑1 (MQ=255)
                                                         tGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTg                                                                                            <  7:186326/148‑1 (MQ=255)
                                                          gtcagcggtcagcgAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTgg                                                                                           <  5:222220/148‑1 (MQ=255)
                                                          gtcagcggtcagcgAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTgg                                                                                           <  7:104968/148‑1 (MQ=255)
                                                           tcagcggtcagcgAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAg                                                                                         >  7:121451/1‑149 (MQ=255)
                                                              gcggtcagcgAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTtg                                                                                      >  7:13108/1‑149 (MQ=255)
                                                               cggtcagcgAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTtgg                                                                                     <  7:150668/149‑1 (MQ=255)
                                                                  tcagcgAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTtggtgg                                                                                  >  2:312016/1‑149 (MQ=255)
                                                                    agcgAAACAAGCTGGGCGATGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGt                                                                                >  8:190300/1‑149 (MQ=255)
                                                                      cgAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTa                                                                              >  7:20929/1‑149 (MQ=255)
                                                                      cgAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTa                                                                              >  8:72499/1‑149 (MQ=255)
                                                                        aaaCACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACa                                                                            <  8:121451/149‑1 (MQ=255)
                                                                        aaaCACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACa                                                                            <  1:312016/149‑1 (MQ=255)
                                                                        aaaCACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACa                                                                            <  4:264979/149‑1 (MQ=255)
                                                                        aaaCACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACa                                                                            >  5:271644/1‑149 (MQ=255)
                                                                                    cgcTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCa                                                               <  6:75213/149‑1 (MQ=255)
                                                                                      cTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACt                                                             <  7:72499/149‑1 (MQ=255)
                                                                                         gCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGt                                                           <  3:310212/148‑1 (MQ=255)
                                                                                          cAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGC‑CGCGCGCACTGTaa                                                         <  3:91796/149‑1 (MQ=255)
                                                                                          cAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGC‑CGCGCGCACTGTaa                                                         <  8:295294/149‑1 (MQ=255)
                                                                                           aaGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAAc                                                        <  7:230351/149‑1 (MQ=255)
                                                                                                gTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGAt                                                   >  3:121418/1‑149 (MQ=255)
                                                                                                gTTCAACGAAAAGAGCCCCTGCATTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGAt                                                   >  7:47767/1‑149 (MQ=255)
                                                                                                       gAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGcc                                            >  7:249298/1‑149 (MQ=255)
                                                                                                          aaGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGc                                         <  8:249298/149‑1 (MQ=255)
                                                                                                            gagCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGAGCG‑GCACTGTAACCCGATCAACGCCGGCGt                                       >  3:43258/1‑149 (MQ=255)
                                                                                                              gCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGc                                     <  8:20929/149‑1 (MQ=255)
                                                                                                               ccccTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGc                                     >  4:38508/1‑148 (MQ=255)
                                                                                                               ccccTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCCTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGc                                     >  8:171264/1‑148 (MQ=255)
                                                                                                                 ccTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGc                                                                                                                                       <  7:236585/49‑1 (MQ=255)
                                                                                                                 ccTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGc                                                                                                                                       >  8:236585/1‑49 (MQ=255)
                                                                                                                  cTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGt                                 >  7:46732/1‑149 (MQ=255)
                                                                                                                  cTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGt                                 <  8:47767/149‑1 (MQ=255)
                                                                                                                     cGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGa                              <  3:38508/149‑1 (MQ=255)
                                                                                                                     cGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGa                              <  7:190300/149‑1 (MQ=255)
                                                                                                                        tATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGAtt                            >  3:290089/1‑148 (MQ=255)
                                                                                                                          tGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACt                         <  7:249323/149‑1 (MQ=255)
                                                                                                                               ggTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCgg                      >  5:8810/1‑147 (MQ=255)
                                                                                                                                 tCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAgc                  <  5:287690/149‑1 (MQ=255)
                                                                                                                                 tCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAgc                  <  5:292558/149‑1 (MQ=255)
                                                                                                                                       cGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCctt             <  4:290089/148‑1 (MQ=255)
                                                                                                                                        gTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCcttc            <  4:43258/148‑1 (MQ=255)
                                                                                                                                           ttCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCcttctgc         <  6:50369/148‑1 (MQ=255)
                                                                                                                                              ttggCCTGGGTAACCCCGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCcttctgcttct     <  3:17688/147‑1 (MQ=255)
                                                                                                                                              ttggCCTGGGTAACCCCGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCcttctgcttct     >  4:17688/3‑149 (MQ=255)
                                                                                                                                              ttggCCTGGGTAACCCCGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCcttctgcttct     >  4:219307/3‑149 (MQ=255)
                                                                                                                                              ttggCCTGGGTAACCCCGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCcttctgcttct     >  1:202480/3‑149 (MQ=255)
                                                                                                                                                ggCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTgg   <  6:271644/149‑1 (MQ=255)
                                                                                                                                                 gCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGa  <  6:214208/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACAAACGAACGTCGTTGCCCTGATGGCTGAATTTAGCCGTCAGGCCGAAATACTTTTTGTCAGCGGTCAGCGAAACACGCTGGGCGCTGGCAAGGCGTTCAACGAAAAGAGCCCCTGCGTTATGCCGGGTCTGTTCGTATTCGGGGCCGGGGTTACCCAGGCCAACGATCAACTGGATGGCGGTCACGTCAGGGGCTCTTCCTTGGAGTTGGTGGGTTACAGTGCGCGCG‑GCACTGTAACCCGATCAACGCCGGCGTGCGAGTGGATTACTCGGCAGCGCCTTCTGCTTCTGGA  >  NC_002947/837778‑838071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑