Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	846,736	G→C	6.1%	T155S (ACT→AGT)	PP_0729 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	846,736	0	G	C	6.1%	249.0 / 3.7	78	T155S (ACT→AGT)	PP_0729	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (37/36);  new base C (2/3);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GTGATCGCGAACTCTATGTCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATACTCCTCTCGCCATCTGCTGCACCAGCACATCATCGCGCTCGCGAGCTAG  >  NC_002947/846589‑846881                                                                                                                                                    |                                                                                                                                                  gTGATCGCGAACTCTATGTCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGt                                                                                                                                                  <  2:15834/149‑1 (MQ=255)  tGATCGCGAACTCTATGTCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCAAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGt                                                                                                                                                  >  5:114618/1‑148 (MQ=255)   gATCGCGAACTCTATGTCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGa                                                                                                                                                >  2:880/1‑149 (MQ=255)    aTCGCGTACTCTATGTCCCAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTATTTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGa                                                                                                                                                <  2:274570/148‑1 (MQ=255)       gcgAACTCTATGTCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCAGTACTGacggt                                                                                                                                            <  2:288130/149‑4 (MQ=255)       gcgAACTCTATGTCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCAGTACTGacggt                                                                                                                                            >  5:102267/1‑146 (MQ=255)        cgAACTCTATGTCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCg                                                                                                                                           >  4:293688/1‑149 (MQ=255)         gAACTCTATGTCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGt                                                                                                                                          >  5:194005/1‑149 (MQ=255)             tctATGTCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAg                                                                                                                                       >  3:10051/1‑148 (MQ=255)               tATGTCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTAc                                                                                                                                    >  4:57280/1‑149 (MQ=255)               tATGTCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTAc                                                                                                                                    >  6:257238/1‑149 (MQ=255)               tATGTCCAAGGCATCGCGCCCGG‑TAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTACTGACACCGTCAGTACt                                                                                                                                   >  7:9244/1‑149 (MQ=255)                   tCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTgg                                                                                                                                 >  6:205892/1‑148 (MQ=255)                   tCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGc                                                                                                                                >  5:245836/1‑149 (MQ=255)                    ccAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGt                                                                                                                                          >  3:48109/1‑138 (MQ=255)                    ccAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGt                                                                                                                                          <  4:48109/138‑1 (MQ=255)                     cAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCa                                                                                                                               <  5:257238/148‑1 (MQ=255)                     cAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCAc                                                                                                                              >  2:158189/1‑149 (MQ=255)                     cAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCAc                                                                                                                              <  1:880/149‑1 (MQ=255)                     cAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCAGTACTGACGGTGTCACTACCGGCaa                                                                                                                              <  6:102267/149‑2 (MQ=255)                     cAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCAGTACTGACGGTGTCACTACCGGCaa                                                                                                                              <  5:278562/149‑2 (MQ=255)                      aaGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCAcc                                                                                                                             >  7:279803/1‑149 (MQ=255)                        ggCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTg                                                                                                                           <  2:49281/149‑1 (MQ=255)                        ggCATCGCGCCCGGTTAGAACTTGTACCGCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTg                                                                                                                           >  1:292850/1‑149 (MQ=255)                         gCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTg                                                                                                                           >  8:141522/1‑148 (MQ=255)                         gCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTg                                                                                                                           <  7:141522/148‑1 (MQ=255)                           aTCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTa                                                                                                                        <  4:10051/149‑1 (MQ=255)                           aTCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTa                                                                                                                        >  6:245721/1‑149 (MQ=255)                               cgcCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATa                                                                                                                    <  3:276134/149‑1 (MQ=255)                               cgcCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATa                                                                                                                    <  3:293688/149‑1 (MQ=255)                               cgcCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATa                                                                                                                    <  8:279803/149‑1 (MQ=255)                                gcCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAg                                                                                                                   >  4:276874/1‑149 (MQ=255)                                   cGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTc                                                                                                                 >  1:157134/1‑148 (MQ=255)                                          aaCTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGt                                                                                                         <  6:114618/149‑1 (MQ=255)                                           aCTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTa                                                                                                        <  7:29510/149‑1 (MQ=255)                                            cTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTaa                                                                                                       <  6:194005/149‑1 (MQ=255)                                            cTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTaa                                                                                                       >  3:256138/1‑149 (MQ=255)                                               gTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAata                                                                                                     <  2:157134/148‑1 (MQ=255)                                               gTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAata                                                                                                     <  1:287258/148‑1 (MQ=255)                                                tACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGTGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATTGTCAACTACGTAatat                                                                                                    <  7:79299/148‑1 (MQ=255)                                                tACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAatat                                                                                                    <  5:205892/148‑1 (MQ=255)                                                  ccTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACg                                                                                                          <  7:280152/140‑1 (MQ=255)                                                  ccTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACg                                                                                                          >  8:280152/1‑140 (MQ=255)                                                   ctctTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGc                                                                                                 >  6:287112/1‑148 (MQ=255)                                                       tCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAAcc                                                                                            >  8:208752/1‑149 (MQ=255)                                                         gAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAAccc                                                                                           <  7:208752/148‑1 (MQ=255)                                                                  aaaCAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGAt                                                                                 >  8:82336/1‑149 (MQ=255)                                                                  aaaCAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGAt                                                                                 <  4:273889/149‑1 (MQ=255)                                                                    acaacCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGAGACCGTCAGTACTGGCACGTGGTAGATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCa                                                                               >  8:123539/1‑149 (MQ=255)                                                                     caacCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGTAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCAc                                                                              <  1:158189/149‑1 (MQ=255)                                                                       acCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACgt                                                                            <  8:101050/149‑2 (MQ=255)                                                                       acCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACgg                                                                            <  3:276874/149‑1 (MQ=255)                                                                       acCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACgg                                                                            <  6:245836/149‑1 (MQ=255)                                                                           tGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCa                                                                        >  4:78061/1‑149 (MQ=255)                                                                                 ttCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCa                                                                  >  3:195328/1‑149 (MQ=255)                                                                                   ccGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACt                                                                >  4:146007/1‑149 (MQ=255)                                                                                     gCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTcc                                                              >  6:255645/1‑149 (MQ=255)                                                                                         gCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTg                                                          >  6:1346/1‑149 (MQ=255)                                                                                                   cGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATActc                                                <  3:146007/149‑1 (MQ=255)                                                                                                     cTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATActcct                                              <  7:82336/149‑1 (MQ=255)                                                                                                       tCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCAGTCTCTGGATCACGGGTCATCGCCACTACGCTGGCGAATActcctc                                             >  8:50584/1‑148 (MQ=255)                                                                                                          cTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATGTGGCAAACCCGTCTCTGGATCACggg                                                                           >  4:268873/1‑115 (MQ=255)                                                                                                          cTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATGTGGCAAACCCGTCTCTGGATCACggg                                                                           <  3:268873/115‑1 (MQ=255)                                                                                                             ccAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCAGGCCAATACTCCTCTCGCCAt                                      >  8:38837/1‑149 (MQ=255)                                                                                                                 gggAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATACTCCTCTCGCCATctgc                                  >  7:12654/1‑149 (MQ=255)                                                                                                                                  acaGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATACTCCTCTCGCCATCTGCTGCACCAGCACatcatc                 >  5:56119/1‑149 (MQ=255)                                                                                                                                  acaGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATACTCCTCTCGCCATCTGCTGCACCAGCACatcatc                 <  4:176688/149‑1 (MQ=255)                                                                                                                                  acaGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATACTCCTCTCGCCATCTGCTGCACCAGCACatcatc                 <  4:195328/149‑1 (MQ=255)                                                                                                                                   caGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGACACTCCGCTGGCCAATACTCCTCTCGCCATctgcagaac                             >  4:269805/1‑131 (MQ=255)                                                                                                                                   caGTATCGTTGCCGGTAGTGACACCGTCAGTACTGGCTCCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATACTCCTCTCGCCATCTGCTGCAc                             <  3:269805/136‑1 (MQ=255)                                                                                                                                    aGTCTCGTTGCCGGTAGTTACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATACTCCTCTCGCCATCTGCTGCACCAGCACATCATcgc               <  7:50584/149‑1 (MQ=255)                                                                                                                                         cGTTGCCGGTAGTGACACCGTCGGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATACTCCTCTCGCCATCTGCTGCACCAGCACATCATCGCGCTcg          >  1:220355/1‑149 (MQ=255)                                                                                                                                              ccGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATACTCCTCTCGCCATCTGCTGCACCAGCACATCATCGCGCTCGCGAg      <  8:12654/148‑1 (MQ=255)                                                                                                                                              ccGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATACTCCTCTCGCCATCTGCTGCACCAGCACATCATCGCGCTCGCGAGc     >  3:222976/1‑149 (MQ=255)                                                                                                                                                 gTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCAc                                                                              >  3:37961/1‑73 (MQ=255)                                                                                                                                                 gTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCAc                                                                              <  4:37961/73‑1 (MQ=255)                                                                                                                                                 gTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATACTCCTCTCGCCATCTGCTGCACCAGCACATCATCGCGCTCGCGAGCTAg  <  3:57280/149‑1 (MQ=255)                                                                                                                                                 gTAGTGACACCGTCAGTACTGGCACCTGGCACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATACTCCTCTCGCCATCTGCTGCACCAGCACATCATCGCGCTCGCGAGCTAg  <  5:287112/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GTGATCGCGAACTCTATGTCCAAGGCATCGCGCCCGGTTAGAACTTGTACCTCTTCGAGCAAAGCAAACAACCCTGGTAATTCCGCAGGCTCGCTTGCCGCTTCGCTGCCAAGGGAGACGAAAGCATGCACAGTCTCGTTGCCGGTAGTGACACCGTCAGTACTGGCACCTGGTACATAGTCAACTACGTAATATGGCAAACCCGTCTCTGGATCACGGGTCATCGCCACTCCGCTGGCCAATACTCCTCTCGCCATCTGCTGCACCAGCACATCATCGCGCTCGCGAGCTAG  >  NC_002947/846589‑846881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑