Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 902,620 A→T 8.7% intergenic (+46/+30) trxB → / ← nadC thioredoxin reductase/quinolinate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947902,6200AT 8.7% 149.6 / 3.4 55intergenic (+46/+30)trxB/nadCthioredoxin reductase/quinolinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (26/24);  new base T (2/3);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GTTTACCGCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGTCGTTTCGTT  >  NC_002947/902479‑902760
                                                                                                                                             |                                                                                                                                            
gTTTACCGCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCgg                                                                                                                                       <  8:82003/149‑1 (MQ=255)
gTTTACCGCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCgg                                                                                                                                       >  7:254105/1‑149 (MQ=255)
     ccGCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGttttt                                                                                                                                  <  4:202521/149‑1 (MQ=255)
      cGCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGtttttt                                                                                                                                 >  3:5007/1‑149 (MQ=255)
         cAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCGCTTGGCCGGTTTTTTAtt                                                                                                                              <  5:260938/149‑1 (MQ=255)
             ccATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCt                                                                                                                          <  5:321581/149‑1 (MQ=255)
             ccATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCt                                                                                                                          >  6:143396/1‑149 (MQ=255)
             ccATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCt                                                                                                                          <  7:284336/149‑1 (MQ=255)
             ccATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCt                                                                                                                          <  2:229661/149‑1 (MQ=255)
                  aCTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAAt                                                                                                                     >  8:265366/1‑149 (MQ=255)
                   cTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTa                                                                                                                                <  1:107801/137‑1 (MQ=255)
                   cTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTa                                                                                                                                >  2:107801/1‑137 (MQ=255)
                             tGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAgg                                                                                                          <  8:254105/149‑1 (MQ=255)
                             tGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAgg                                                                                                          <  6:227773/149‑1 (MQ=255)
                                   cTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGt                                                                                                    >  4:139225/1‑149 (MQ=255)
                                      cATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTc                                                                                                               >  2:160977/1‑135 (MQ=255)
                                      cATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTc                                                                                                               <  1:160977/135‑1 (MQ=255)
                                       aTGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCa                                                                                                <  6:105054/149‑1 (MQ=255)
                                        tGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCAt                                                                                               <  6:95049/149‑1 (MQ=255)
                                            ggcgCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGa                                                                                            >  4:225493/1‑148 (MQ=255)
                                            ggcgCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGAc                                                                                           <  3:139225/149‑1 (MQ=255)
                                                 tGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTc                                                                                      >  4:3530/1‑149 (MQ=255)
                                                      gTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGc                                                                                                                            <  2:223817/106‑1 (MQ=255)
                                                      gTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGc                                                                                                                            >  1:223817/1‑106 (MQ=255)
                                                       tCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGc                                                                                <  1:6789/149‑1 (MQ=255)
                                                        cGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAg                                                                                                           >  3:185388/1‑121 (MQ=255)
                                                        cGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAg                                                                                                           <  4:185388/121‑1 (MQ=255)
                                                         gAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCt                                                                               >  4:300995/1‑148 (MQ=255)
                                                         gAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCt                                                                               <  3:300995/148‑1 (MQ=255)
                                                                      aCGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCTCATCGACAGGTCCACCGCTTTCACATCCTTGGt                                                                 <  2:300759/149‑1 (MQ=255)
                                                                            tGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATgg                                                            <  5:147305/148‑1 (MQ=255)
                                                                            tGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATgg                                                            <  3:3530/148‑1 (MQ=255)
                                                                              gCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCAc                                                         <  4:262552/149‑1 (MQ=255)
                                                                                 aaCGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCaa                                                      <  2:33401/149‑1 (MQ=255)
                                                                                   cGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATc                                                    >  6:133051/1‑149 (MQ=255)
                                                                                       tCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCtt                                                                    <  4:303538/129‑1 (MQ=255)
                                                                                       tCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCtt                                                                    >  3:303538/1‑129 (MQ=255)
                                                                                               atctgaGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTc                                         >  8:284041/1‑148 (MQ=255)
                                                                                                   gagaAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTGTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCAccc                                    >  3:32996/1‑149 (MQ=255)
                                                                                                    agaAAACGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGGGCCGGTTTTGTTTTGCCGGCCTTCAGCTCACAGGCTCAGTCGCGGCGACAGGTCGCCCGCGTGCACAGGCGTGGTCAGGGCGCCGCTCGAGCTGTAGTCcccccc                                   >  2:209079/1‑149 (MQ=255)
                                                                                                              gTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGc                          >  6:49733/1‑148 (MQ=255)
                                                                                                                  aaaGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCa                     >  6:256297/1‑149 (MQ=255)
                                                                                                                           ccAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAggg            >  7:137144/1‑149 (MQ=255)
                                                                                                                             aaTAAAAAACCGGCGCTTGGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTg                                                                   <  1:128516/92‑1 (MQ=37)
                                                                                                                             aaTAAAAAACCGGCGCTTGGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTg                                                                   >  2:128516/1‑92 (MQ=37)
                                                                                                                             aaTAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtc          >  8:115521/1‑149 (MQ=255)
                                                                                                                              aTAAAAAACCGGCGCTTGGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCAc                                                                                   >  6:92936/1‑75 (MQ=25)
                                                                                                                              aTAAAAAACCGGCGCTTGGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCAc                                                                                   <  5:92936/75‑1 (MQ=25)
                                                                                                                              aTAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtcg         >  4:180580/1‑149 (MQ=255)
                                                                                                                                 aaaaaCCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtcgttt      >  7:9410/1‑149 (MQ=255)
                                                                                                                                   aaaCCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtcgtttcg    >  7:178245/1‑149 (MQ=255)
                                                                                                                                   aaaCCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtcgtttcg    >  5:143678/1‑149 (MQ=255)
                                                                                                                                    aaCCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtcgtttcgt   <  3:225493/149‑1 (MQ=255)
                                                                                                                                    aaCCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCAACAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGgtcgtgtcgt   >  6:300946/1‑149 (MQ=255)
                                                                                                                                     aCCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtcgtttcgtt  <  5:133051/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
GTTTACCGCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGTCGTTTCGTT  >  NC_002947/902479‑902760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑