Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	142,401	C→A	10.4%	T115T (ACC→ACA)	PP_0134 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	142,401	0	C	A	10.4%	161.3 / 7.1	57	T115T (ACC→ACA)	PP_0134	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (26/25);  new base A (3/3);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

AACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGCCAAGGAGCTGGCGGGCGTGGTAGCGCGTA  >  NC_002947/142255‑142549                                                                                                                                                   |                                                                                                                                                     aaCGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCt                                                                                                                                                    >  2:227510/1‑149 (MQ=255)   cGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTgg                                                                                                                                                  >  2:302792/1‑149 (MQ=255)         cGAGCGGCAGCTGGCCGAGGATGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGcggcgg                                                                                                                                            <  7:57070/149‑1 (MQ=255)          gAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGt                                                                                                                                           >  4:278930/1‑149 (MQ=255)          gAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGt                                                                                                                                           <  3:229464/149‑1 (MQ=255)          gAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGt                                                                                                                                           >  7:248085/1‑149 (MQ=255)              ggCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATc                                                                                                                                       <  5:173631/149‑1 (MQ=255)               gCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATcc                                                                                                                                      >  1:128924/1‑149 (MQ=255)                 aGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGGAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGt                                                                                                                                    >  3:36948/1‑149 (MQ=255)                   cTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGc                                                                                                                                  <  4:172908/149‑1 (MQ=255)                       ccGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCgg                                                                                                                              <  3:278930/149‑1 (MQ=255)                         gAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCt                                                                                                                            <  3:271035/149‑1 (MQ=255)                               gTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTtgtg                                                                                                                      <  4:254531/149‑1 (MQ=255)                                 ggCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTtgtg                                                                                                                      >  3:262021/1‑147 (MQ=255)                                  gCCCGTGCTACCCGCGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGAAGTGACCCTCGCGGCGGTGATCCGTGCACGGCTGTTGTGCAg                                                                                                                   >  6:3502/1‑149 (MQ=255)                                    ccGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGcc                                                                                                                 >  6:261598/1‑149 (MQ=255)                                             ccGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCg                                                                                                        <  4:262021/149‑1 (MQ=255)                                                 ggCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGGAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGaaaa                                                                                                    <  7:268925/149‑1 (MQ=255)                                                  gCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAg                                                                                                   <  8:115268/149‑1 (MQ=255)                                                       gAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCAcc                                                                                              >  4:196580/1‑149 (MQ=255)                                                       gAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCAc                                                                                               <  1:302792/148‑1 (MQ=255)                                                             gTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAg                                                                                         >  6:30792/1‑148 (MQ=255)                                                                gagaACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGt                                                                                     <  8:19926/149‑1 (MQ=255)                                                                              gCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAgg                                                                       <  7:229557/149‑1 (MQ=255)                                                                              gCTAAATGCCCTTCTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTTCGCCCAGCGGCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCGGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAgg                                                                       <  3:108718/149‑1 (MQ=255)                                                                               cTCAATGCCCAACTGGTGGAGCGTCCGCAGGATCTGCGTGCCTACGCCCAGCGCCTGGATGACGTGACCCTGGCGGCGGTTATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAg                                                                        <  5:3502/147‑1 (MQ=255)                                                                                  aaTGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGgt                                                                     <  3:196580/147‑1 (MQ=255)                                                                                   aTGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGtgtg                                                                  <  5:261598/149‑1 (MQ=255)                                                                                       ccAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGa                                                              >  5:109466/1‑149 (MQ=255)                                                                                            tggtggAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACACTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAATGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTg                                                         >  6:186828/1‑149 (MQ=255)                                                                                            tggtggAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACACTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTg                                                         >  6:306573/1‑149 (MQ=255)                                                                                            tggtggAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACACTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTg                                                         >  6:191336/1‑149 (MQ=255)                                                                                              gtggAGCGTCCCCAGGAGCAGCGTGCCTACCCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGAGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCCAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGCCGTTgag                                                       >  2:35759/1‑147 (MQ=255)                                                                                               tggAGCGTCCGCGGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGt                                                      >  8:299365/1‑149 (MQ=255)                                                                                                     gTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAg                                                <  5:30792/149‑1 (MQ=255)                                                                                                         gCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGACGTACAACCGATAAGGGACCTATAGAAGTAAAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTc                                             >  1:265895/1‑148 (MQ=255)                                                                                                          cAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGc                                                        >  3:138686/1‑136 (MQ=255)                                                                                                          cAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGc                                                        <  4:138686/136‑1 (MQ=255)                                                                                                          cAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACACTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGa                                           <  5:186828/149‑1 (MQ=255)                                                                                                          cAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACACTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGa                                           <  5:306573/149‑1 (MQ=255)                                                                                                          cAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACACTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGa                                           <  5:191336/149‑1 (MQ=255)                                                                                                                 tGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGAGcc                                    >  4:98090/1‑149 (MQ=255)                                                                                                                   cGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGcc                                    <  8:248085/147‑1 (MQ=255)                                                                                                                        cTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGcc                              >  3:181036/1‑148 (MQ=255)                                                                                                                        cTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGcc                              >  1:259285/1‑148 (MQ=255)                                                                                                                         tACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGcc                              >  7:57746/1‑147 (MQ=255)                                                                                                                            gCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGCCAAg                           >  3:171942/1‑147 (MQ=255)                                                                                                                              ccAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGTGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGCCAAGGAGc                       >  7:126870/1‑149 (MQ=255)                                                                                                                              ccAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGCCAAGGAGc                       >  2:273087/1‑149 (MQ=255)                                                                                                                                 gcgcCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGCCAAGGAGCTgg                    <  4:36948/149‑1 (MQ=255)                                                                                                                                          ggaCGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGCCAAGGAGCTGGCGGGCGTgg           <  6:109466/149‑1 (MQ=255)                                                                                                                                           gaCGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGCCAAGGAGCTGGCGGGCGTGGt          <  2:41453/149‑1 (MQ=255)                                                                                                                                             cGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGCCAAGGAGCTGGCGGGCGTGGTAg        >  6:134640/1‑149 (MQ=255)                                                                                                                                              gTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGCCAAGGAGCTGGCGGGCGt             <  6:39392/143‑1 (MQ=255)                                                                                                                                              gTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGCCAAGGAGCTGGCGGGCGt             >  5:39392/1‑143 (MQ=255)                                                                                                                                              gTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGCCAAGGAGCTGGCGGGCGTGGTAgc       <  7:299365/149‑1 (MQ=255)                                                                                                                                                 aCCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGCCAAGGAGCTGGCGGGCGTGGTAGcgcg    >  1:136020/1‑149 (MQ=255)                                                                                                                                                   ccTGGCTGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGTTGTGGTGACGTTGCGTGGCAAGGTCGACCGTGCCGAAGCCAAGGAGCTGGCGCGCGTGGTAGCGCGTa  >  8:89884/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                     AACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAGAGAACCTGCTGCAGCTCAATGCCCAACTGGTGGAGCGTCCGCAGGAGCTGCGTGCCTACGCCCAGCGCCTGGAGGACGTGACCCTGGCGGCGGTGATCCGTGCTCGGCTGTTGTGGAGCCGTACCACCGAAAAGGCACCTATAGAAGTACAGAGCAGCGAGGGTGTGGTGACGTTGCGTGGCAAGGTCGACAGTGCCGAAGCCAAGGAGCTGGCGGGCGTGGTAGCGCGTA  >  NC_002947/142255‑142549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑