| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 955,153 | T→C | 26.7% | intergenic (+107/‑206) | cyoE → / → alaC | protoheme IX farnesyltransferase/aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 955,153 | 0 | T | C | 26.7% | 42.2 / 15.7 | 30 | intergenic (+107/‑206) | cyoE/alaC | protoheme IX farnesyltransferase/aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (12/10); new base C (2/6); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCA > NC_002947/955006‑955296 | gCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTc < 7:105803/149‑1 (MQ=255) aCCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTa < 5:75603/149‑1 (MQ=255) ccGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAg < 1:130599/149‑1 (MQ=255) cGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAgg > 5:17953/1‑149 (MQ=255) cGGATTTGCTTATGACCTACGCACGATGAAACTGCTGCCTGTTTCACGAAAACCCCGTCCACTGTGCCGGTGTTTTTTTTTGGTGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCgg < 6:17953/149‑1 (MQ=38) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGc < 4:252292/147‑1 (MQ=38) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCgg < 1:320700/149‑1 (MQ=255) ggATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCggg < 5:71430/149‑1 (MQ=37) ggATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCggg < 5:214613/149‑1 (MQ=37) ggATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCggg < 8:192098/149‑1 (MQ=37) tgtgCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGttt < 4:58789/149‑1 (MQ=255) gatgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGt > 1:299680/1‑131 (MQ=255) gatgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGt < 2:299680/131‑1 (MQ=255) agcctcaaaCTCCTGCCTTTCTCACCAAACCCCCGGCCACTGGGCGGGGTTTTTTTTTTGGGGTTTTTTTCCCTGTGCCGGCCTCTTCGCGGGTAACCCCGCTCCAACAAGGTTCCTGTTGGAGGGGGTTAACCCGCGAAGAGGCCa < 2:271772/141‑1 (MQ=21) ccTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTGCTACAAGGTTGCTGTTGGAGCGGGTTTTCCCGCGAaga > 5:171494/1‑127 (MQ=255) ccGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCggg > 7:306175/1‑96 (MQ=18) ccGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGGAGCggg < 8:306175/96‑1 (MQ=18) cGGGGTTTTTTTTTGGGGGTATTTTTCATGTGCCGGCCTCTTCGCGGGTAAAACCCCTACTAAAAGGTTACTGTAGGAGCGGGTTTAACCGCGAAGAGGCCAGCACAGAAAAATAAAAATACAATAATTTGAAAAAATAGAAATGaaa > 6:92984/1‑148 (MQ=25) ttCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTAAAAAAATAGATCTGAAAAGTTATAATAAAACAGGAAATTATCATTTACAGGCATACTg > 8:9566/1‑149 (MQ=255) aaaCCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCa < 5:66386/148‑1 (MQ=255) aaaCCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCa > 6:66386/1‑148 (MQ=255) cccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAt > 1:302560/1‑149 (MQ=255) ccGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGAATCCTGTTTCGGCACTAtc > 7:148989/1‑149 (MQ=255) cGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCtaa < 4:34960/102‑1 (MQ=21) cGCTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCtaa > 3:34960/1‑102 (MQ=21) tACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAt > 5:312124/1‑149 (MQ=255) aCAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAt > 8:164840/1‑148 (MQ=255) cAAGGTTCCTGTAGGAGCGGCTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACATGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAAATTTCCTTTACAGGCATCATGTTGCGGCACTAGCATGTGAtt > 4:88658/1‑148 (MQ=38) aatgTTCCTTTAGTATCTTGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCa < 5:92984/146‑1 (MQ=255) aaGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAtt > 4:170682/1‑147 (MQ=255) | GCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGATTCA > NC_002947/955006‑955296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |