Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 956,584 A→T 8.0% intergenic (+17/‑97) alaC → / → PP_0818 aminotransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947956,5840AT 8.0% 140.0 / 3.3 50intergenic (+17/‑97)alaC/PP_0818aminotransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/24);  new base T (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

TGACTATGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTACTTCTGCGGCACTGGCTCGCACCGTTTCGAC  >  NC_002947/956453‑956731
                                                                                                                                    |                                                                                                                                                   
tGACTATGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCct                                                                                                                                    >  1:19195/1‑149 (MQ=255)
  aCTATGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCTTCGCAGCCCCattg                                                                                                                                        <  7:232964/143‑4 (MQ=255)
  aCTATGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCTTCGCAGCCCCattg                                                                                                                                        >  8:232964/1‑140 (MQ=255)
      tGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTggg                                                                                                                              <  3:130290/149‑1 (MQ=255)
      tGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTgg                                                                                                                               <  1:69342/148‑1 (MQ=255)
      tGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTgg                                                                                                                               <  4:78936/148‑1 (MQ=255)
       ggggACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGt                                                                                                                             >  6:268471/1‑149 (MQ=255)
         ggACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGa                                                                                                                           <  3:226770/149‑1 (MQ=255)
         ggACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCAATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGa                                                                                                                           <  3:222554/149‑1 (MQ=255)
           acgacCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGc                                                                                                                         >  6:330029/1‑149 (MQ=255)
             gacCATGTGCGCTTTGCGTTGCTCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTgg                                                                                                                               >  1:82628/1‑141 (MQ=255)
             gacCATGGGCGCTTTGCGTTGTTCGATAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTgg                                                                                                                               <  2:82628/141‑1 (MQ=255)
               ccATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTc                                                                                                                     >  7:223491/1‑149 (MQ=255)
                    tgCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCgcat                                                                                                                >  1:289543/1‑147 (MQ=255)
                              gTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGt                                                                                                      >  3:160301/1‑149 (MQ=255)
                                  aTCGAGATCCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTAc                                                                                                   <  5:330029/148‑1 (MQ=255)
                                  aTCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGCAAAATGTTCCGGGCTGATGGTTTGGATCGCAATTACGCCCAATGGGGCTGCGAAGCAGCCCCAACTCCATCTTGGTTGCGATTTCCGCATGCGTACTGGTTAc                                                                                                   >  1:75753/1‑149 (MQ=255)
                                  aTCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTAc                                                                                                   <  8:61703/148‑1 (MQ=255)
                                       gaACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTAc                                                                                             <  5:268471/149‑1 (MQ=255)
                                           cAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCt                                                                                                         <  6:288308/133‑1 (MQ=255)
                                           cAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCt                                                                                                         >  5:288308/1‑133 (MQ=255)
                                             ggACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTAc                                                                                       <  5:295877/149‑1 (MQ=255)
                                              gACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTAcc                                                                                      <  7:189529/149‑1 (MQ=255)
                                                 cGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTc                                                                                   <  3:129878/149‑1 (MQ=255)
                                                  gTATTCGCCAGGCCATCGGCGGGATACGGC‑AAATGTTCCGGGCTGTTGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCa                                                                                  <  8:167641/149‑1 (MQ=255)
                                                    tttCGCCTGGCCTGCGGGGGGATCCGGA‑AAATGTTCGGGGCTGTGGGTTGGGTGCGCAAGTAAGCCCATTGGGGGTGCTAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAg                                                                                 <  4:160301/147‑1 (MQ=255)
                                                        gCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACt                                                                             >  7:8352/1‑148 (MQ=255)
                                                          cAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCAt                                                                          <  2:19195/149‑1 (MQ=255)
                                                                aTCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCa                                                                    >  7:50670/1‑149 (MQ=255)
                                                                   cgcgGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCAcc                                                                  >  5:288515/1‑148 (MQ=255)
                                                                     cgGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTg                                                                                                                                >  5:261971/1‑84 (MQ=255)
                                                                     cgGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTg                                                                                                                                <  6:261971/84‑1 (MQ=255)
                                                                     cgGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCaaa                                                               <  8:50670/149‑1 (MQ=255)
                                                                         aTCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCaaa                                                               >  7:78570/1‑145 (MQ=255)
                                                                             ggC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAGGAGATccc                                                       <  6:288515/149‑1 (MQ=255)
                                                                              gC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCTTCGCAGCCCCatt                                                                                                                                         <  4:21857/66‑3 (MQ=37)
                                                                              gC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCTTCGCAGCCCCatt                                                                                                                                         >  3:21857/1‑64 (MQ=37)
                                                                                      ttCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAgt                                               <  8:78570/149‑1 (MQ=255)
                                                                                      ttCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTATCACCAAAGAGATCACCCATGact                                               >  8:264806/1‑147 (MQ=255)
                                                                                             cTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTAc                                                                                             >  6:174005/1‑96 (MQ=255)
                                                                                             cTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTAc                                                                                             <  5:174005/96‑1 (MQ=255)
                                                                                              tGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCAc                                       >  8:74106/1‑149 (MQ=255)
                                                                                              tGATGGTTTGCCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACCCCAACCGTCAg                                                                                 >  7:54264/1‑107 (MQ=255)
                                                                                                                 tAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTACTTCTGCGGCACt                    >  3:228260/1‑149 (MQ=255)
                                                                                                                          tGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTACTTCTGCGGCACTGGCTCGCAc           <  2:75753/149‑1 (MQ=255)
                                                                                                                              gCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCCCCGCCTACTTCTGCGGCGCTGGCTCGCCCCGtt       >  4:56474/1‑149 (MQ=255)
                                                                                                                              gCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCt                                  <  2:221532/122‑1 (MQ=255)
                                                                                                                              gCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCt                                  >  1:221532/1‑122 (MQ=255)
                                                                                                                                  cGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTACTTCTGCGGCACTGGCTCGCACCGTTTc     <  7:74106/147‑1 (MQ=255)
                                                                                                                                   gAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCCCTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTACTTCTGCGGCACTGGCTCGCACCGTTTcgac  >  5:239941/1‑149 (MQ=255)
                                                                                                                                    |                                                                                                                                                   
TGACTATGGGGACGACCATGTGCGCTTTGCGTTGATCGAGAACCAGGACCGTATTCGCCAGGCCATCCGCGGGATCCGGC‑AAATGTTCCGGGCTGATGGTTTGGCTCGCAAGTAAGCCCAATGGGGCTGCGAAGCAGCCCCAACTGCCTCTTGGGTGAGCTTTCCGCCTGCCTTCTGGTTACCCGTACGCCTACCGTCAGCACTCATTTCTCACCAAAGAGATCCCCCATGAGTGTCTTCACCGCCTACTTCTGCGGCACTGGCTCGCACCGTTTCGAC  >  NC_002947/956453‑956731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑