Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	983,263	G→A	10.0%	A92T (GCT→ACT) ‡	fdx →	ferredoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	983,263	0	G	A	10.0%	103.9 / 2.7	40	A92T (GCT→ACT) ‡	fdx	ferredoxin
Reads supporting (aligned to +/- strand):  ref base G (20/16);  new base A (2/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01

TCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAAAGCAAGCCTGAGGTC  >  NC_002947/983123‑983407                                                                                                                                             |                                                                                                                                                 tCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGatg                                                                                                                                            >  2:306558/1‑145 (MQ=255)   aaGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTCTTCGTCAGCGACGaaaa                                                                                                                                        >  4:293314/1‑147 (MQ=255)    aGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTCTTCGTCAGCGACGAAAAc                                                                                                                                       <  3:293314/149‑1 (MQ=255)      gCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACAAGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCt                                                                                                                                     >  2:136669/1‑149 (MQ=255)        cTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTCTTCGTCAGCGACGAAAACCTgg                                                                                                                                   <  1:45791/149‑2 (MQ=255)             ccACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATc                                                                                                                              <  1:316900/149‑1 (MQ=255)                        tGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCt                                                                                                                                                >  6:56972/1‑120 (MQ=255)                        tGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCt                                                                                                                                                <  5:56972/120‑1 (MQ=255)                        tGCATCGTGCGCAACGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGCAAGAGGACATGCTGGACAAAGCCTGGGGCCTCGAAGCGCAATCGCGCCTGCGTTGCCAGGATATCCTCGCTGACGAAGACCTGACCAACTAGATcccc                                                                                                                     >  2:198863/1‑147 (MQ=255)                          cATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCaa                                                                                                                   <  6:116939/147‑1 (MQ=255)                          cATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCaa                                                                                                                   <  5:34747/147‑1 (MQ=255)                               tGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCg                                                                                                            <  8:102708/149‑1 (MQ=255)                                          ttCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCAtgc                                                                                                 >  6:57548/1‑149 (MQ=255)                                           tCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCAtgct                                                                                                >  6:11439/1‑149 (MQ=255)                                                  tcTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAg                                                                                         >  8:298701/2‑149 (MQ=255)                                                  gCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAg                                                                                         <  3:57524/149‑1 (MQ=255)                                                       aaGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCg                                                                                    >  3:210858/1‑149 (MQ=255)                                                             cTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGa                                                                              <  7:298701/149‑1 (MQ=255)                                                                aCGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAg                                                                             <  5:11439/147‑1 (MQ=255)                                                                       ggAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTa                                                                     >  3:233491/1‑148 (MQ=255)                                                                          agatGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATg                                                                 <  7:205020/149‑1 (MQ=255)                                                                               acttggtGGAAAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGTGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCGCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTc                                                             <  5:326046/142‑1 (MQ=255)                                                                                   gCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGaaa                                                        >  3:251994/1‑149 (MQ=255)                                                                                    cTGGACATAGGGTGGGGCCTTGAAGCGCAATCGCGGCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCTTCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAAt                                                       <  1:198863/149‑1 (MQ=255)                                                                                     tGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATg                                                      >  7:36930/1‑149 (MQ=255)                                                                                        aCAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGt                                                   <  4:233491/149‑1 (MQ=255)                                                                                          aaaGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGtt                                                  >  8:27898/1‑148 (MQ=255)                                                                                                       tGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGACGCTGACGAAGACCTTACCATCCAGATCCCCAAGTATTCGCTCAACAATCATGCCGAAGCACCCCACTGAGGGCAAGTACATGAGTCTGAAATGGGTTGATGTACtt                                         >  4:285981/1‑144 (MQ=255)                                                                                                            gcgcAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCaa                               >  2:93010/1‑149 (MQ=255)                                                                                                                    gcgcCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCt                        <  4:251994/148‑1 (MQ=255)                                                                                                                          gggTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGa                  >  5:310358/1‑148 (MQ=255)                                                                                                                           ggTTGCCAGGTTTTCGTCGCTGATGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGaaa                <  1:306558/149‑1 (MQ=255)                                                                                                                              ggtcAGGTCTTCGTCAGCGACGAAAACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagc              >  4:165765/4‑148 (MQ=255)                                                                                                                                ccAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATcc                            <  2:311087/132‑1 (MQ=255)                                                                                                                                ccAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATcc                            >  1:311087/1‑132 (MQ=255)                                                                                                                                ccAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagcaag           >  2:322230/1‑149 (MQ=255)                                                                                                                                ccAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATCAGTCTGAAATGCGTTGATGTACTTGAGATCGCAATCCAGCTTTCAGAaagcaag           >  7:132686/1‑149 (MQ=255)                                                                                                                                 cAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagcaagc          >  5:4110/1‑149 (MQ=255)                                                                                                                                 cAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagcaagc          >  8:162031/1‑149 (MQ=255)                                                                                                                                 cAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagcaagc          <  8:36930/149‑1 (MQ=255)                                                                                                                                         cgtcgCTGACGAAGACCTGACCATCGAGATCCCCAAGAATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAAAGCAAGCCTGAGGTc  >  1:58782/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                 TCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAAAGCAAGCCTGAGGTC  >  NC_002947/983123‑983407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑