Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	983,265	T→C	10.0%	A92A (GCT→GCC) ‡	fdx →	ferredoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	983,265	0	T	C	10.0%	102.1 / 2.9	40	A92A (GCT→GCC) ‡	fdx	ferredoxin
Reads supporting (aligned to +/- strand):  ref base T (20/16);  new base C (2/2);  total (22/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

TCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAAAGCAAGCCTGAGGTCGATC  >  NC_002947/983123‑983411                                                                                                                                               |                                                                                                                                                    tCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGatg                                                                                                                                                 >  2:306558/1‑145 (MQ=255)   aaGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTCTTCGTCAGCGACGaaaa                                                                                                                                             >  4:293314/1‑147 (MQ=255)    aGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTCTTCGTCAGCGACGAAAAc                                                                                                                                            <  3:293314/149‑1 (MQ=255)      gCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACAAGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCt                                                                                                                                          >  2:136669/1‑149 (MQ=255)        cTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTCTTCGTCAGCGACGAAAACCTgg                                                                                                                                        <  1:45791/149‑2 (MQ=255)             ccACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATc                                                                                                                                   <  1:316900/149‑1 (MQ=255)                        tGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCt                                                                                                                                                     >  6:56972/1‑120 (MQ=255)                        tGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCt                                                                                                                                                     <  5:56972/120‑1 (MQ=255)                        tGCATCGTGCGCAACGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGCAAGAGGACATGCTGGACAAAGCCTGGGGCCTCGAAGCGCAATCGCGCCTGCGTTGCCAGGATATCCTCGCTGACGAAGACCTGACCAACTAGATcccc                                                                                                                          >  2:198863/1‑147 (MQ=255)                          cATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCaa                                                                                                                        <  5:34747/147‑1 (MQ=255)                          cATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCaa                                                                                                                        <  6:116939/147‑1 (MQ=255)                               tGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCg                                                                                                                 <  8:102708/149‑1 (MQ=255)                                          ttCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCAtgc                                                                                                      >  6:57548/1‑149 (MQ=255)                                           tCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCAtgct                                                                                                     >  6:11439/1‑149 (MQ=255)                                                  tcTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAg                                                                                              >  8:298701/2‑149 (MQ=255)                                                  gCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAg                                                                                              <  3:57524/149‑1 (MQ=255)                                                       aaGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCg                                                                                         >  3:210858/1‑149 (MQ=255)                                                             cTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGa                                                                                   <  7:298701/149‑1 (MQ=255)                                                                aCGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAg                                                                                  <  5:11439/147‑1 (MQ=255)                                                                       ggAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTa                                                                          >  3:233491/1‑148 (MQ=255)                                                                          agatGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATg                                                                      <  7:205020/149‑1 (MQ=255)                                                                               acttggtGGAAAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGTGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCGCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTc                                                                  <  5:326046/142‑1 (MQ=255)                                                                                   gCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGaa‑a                                                            >  3:251994/1‑149 (MQ=255)                                                                                    cTGGACATAGGGTGGGGCCTTGAAGCGCAATCGCGGCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCTTCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑At                                                           <  1:198863/149‑1 (MQ=255)                                                                                     tGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATg                                                          >  7:36930/1‑149 (MQ=255)                                                                                        aCAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGt                                                       <  4:233491/149‑1 (MQ=255)                                                                                          aaaGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGtt                                                      >  8:27898/1‑148 (MQ=255)                                                                                                       tGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGACGCTGACGAAGACCTTACCATCCAGATCCCCAAGTATTCGCTCAACAATCATGCCGAAGCACCCCACTGAGGGCAAGTACATGAGTCTGAA‑ATGGGTTGATGTACtt                                             >  4:285981/1‑144 (MQ=255)                                                                                                            gcgcAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCaa                                   >  2:93010/1‑149 (MQ=255)                                                                                                                    gcgcCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCAATCCAGCt                            <  4:251994/148‑1 (MQ=255)                                                                                                                          gggTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGa                      >  5:310358/1‑148 (MQ=255)                                                                                                                           ggTTGCCAGGTTTTCGTCGCTGATGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGaaa                    <  1:306558/149‑1 (MQ=255)                                                                                                                              ggtcAGGTCTTCGTCAGCGACGAAAACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagc                  >  4:165765/4‑148 (MQ=255)                                                                                                                                ccAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCAATcc                                <  2:311087/132‑1 (MQ=255)                                                                                                                                ccAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCAATcc                                >  1:311087/1‑132 (MQ=255)                                                                                                                                ccAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagcaag               >  2:322230/1‑149 (MQ=255)                                                                                                                                ccAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATCAGTCTGAA‑ATGCGTTGATGTACTTGAGATCGCAATCCAGCTTTCAGAaagcaag               >  7:132686/1‑149 (MQ=255)                                                                                                                                 cAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagcaagc              >  5:4110/1‑149 (MQ=255)                                                                                                                                 cAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagcaagc              >  8:162031/1‑149 (MQ=255)                                                                                                                                 cAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagcaagc              <  8:36930/149‑1 (MQ=255)                                                                                                                                         cgtcgCTGACGAAGACCTGACCATCGAGATCCCCAAGAATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAAAGCAAGCCTGAGGTc      >  1:58782/1‑149 (MQ=255)                                                                                                                                              cTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAACTTGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAAAGCAAGCCTGAGGTCGATc  <  1:93010/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    TCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAA‑ATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAAAGCAAGCCTGAGGTCGATC  >  NC_002947/983123‑983411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑