Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,013,765 C→G 6.1% V206V (GTG→GTC) ‡ potF‑I ← putrescine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,013,7650CG 6.1% 203.8 / 2.9 65V206V (GTG→GTC) ‡potF‑Iputrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (32/29);  new base G (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

CCCACCTTCACGCCATTGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCATTGTCCAGCACCGCGACGCCACAGCTTTTCAGCTTGCTCATGTACT  >  NC_002947/1013622‑1013913
                                                                                                                                               |                                                                                                                                                    
cccACCTTCACGCCATTGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACttt                                                                                                                                                 >  5:253280/1‑149 (MQ=255)
 ccACCTTCACGCCATTGTTCGCCTCGTCTGCGCGGTTCTTCGCCTGCAGCACATCCCCTTAGAACCCCACTACCACGCAGATATCGCCGTTGGCCATGTAGCCCGTTTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTtc                                                                                                                                                <  4:4226/149‑1 (MQ=255)
 ccACCTTCACGCCATTGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACttt                                                                                                                                                 >  1:131470/1‑148 (MQ=255)
 ccACCTTCACGCCATTGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACttt                                                                                                                                                 >  7:241456/1‑148 (MQ=255)
 ccACCTTCACGCCATTGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTtc                                                                                                                                                <  3:198438/149‑1 (MQ=255)
      ttCACGCCATTGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATcag                                                                                                                                           >  5:232575/1‑149 (MQ=255)
      ttCACGCCATTGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATcag                                                                                                                                           >  8:14744/1‑149 (MQ=255)
      ttCACGCCATTGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATcag                                                                                                                                           >  3:242989/1‑149 (MQ=255)
        cACGCCATTGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGAAAGTGCGGCCTTACATcagc                                                                                                                                          <  7:25707/148‑1 (MQ=255)
           gCCATTGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTtcatc                                                                                                                                             >  4:87434/1‑142 (MQ=255)
           gCCATTGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTtcatc                                                                                                                                             <  3:87434/142‑1 (MQ=255)
            ccATTGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGAAAGTGCGGCCTTACATcagctattt                                                                                                                                     <  8:279889/149‑6 (MQ=38)
               ttGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCtt                                                                                                                                  <  5:12806/149‑1 (MQ=255)
                 gTTCGGCTCGTCGGCGCGTTTCTTCGCCTTCAGCACATCCCCTGAGAACACCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTTTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTg                                                                                                                                 <  2:131470/148‑1 (MQ=255)
                 gTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTg                                                                                                                                 <  8:241456/148‑1 (MQ=255)
                     gCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCt                                                                                                                             >  6:187297/1‑148 (MQ=255)
                       cTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTtct                                                                                                                          >  2:146506/1‑149 (MQ=255)
                        tcgtcgGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTtctc                                                                                                                         >  7:66421/1‑149 (MQ=255)
                        tagtcgGCGCGGATGGTCGCCTGCCGCAAATCCCCTGAGCAGCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGGGTAGTTGGACGAGTGGATATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGGCCTTGGCCTtctc                                                                                                                         >  6:188651/3‑149 (MQ=255)
                         cggcgGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCGGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGTCCTTCTCg                                                                                                                        <  5:188651/146‑1 (MQ=255)
                          gtcgGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCg                                                                                                                        <  5:41954/148‑1 (MQ=255)
                           tcgGCTCGTTTCTTCGCCTTCAGCACATCCCCTGAGAACCCCCCTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGGGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTa                                                                                                                      <  6:327740/149‑1 (MQ=255)
                                  ggTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTg                                                                                                               >  5:256626/1‑149 (MQ=255)
                                    ttcttcGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTggg                                                                                                             <  5:238216/149‑1 (MQ=255)
                                     tcttcGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCGTTGGCCTTCTCGTAGTCCTTgtgg                                                                                                            >  5:286722/1‑149 (MQ=255)
                                       ttcGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTg                                                                                                               <  3:188894/144‑1 (MQ=255)
                                       ttcGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTg                                                                                                               >  4:188894/1‑144 (MQ=255)
                                              gcagcaCATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCtg                                                                                                   >  6:27628/1‑149 (MQ=255)
                                               cagcaCATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCtgt                                                                                                  <  5:187297/149‑1 (MQ=255)
                                                agcaCATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCtgtg                                                                                                 <  4:242989/149‑1 (MQ=255)
                                                agcaCATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCtgta                                                                                                 >  3:180872/1‑148 (MQ=255)
                                                agcaCATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCtg                                                                                                   <  4:180872/147‑1 (MQ=255)
                                                    caTCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATgg                                                                                             <  6:256626/149‑1 (MQ=255)
                                                    caTCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGATGGTCCTTGGCCTTAACGTAGTCCTTGGGGTCTTGGATGTTATgg                                                                                             >  3:234411/1‑149 (MQ=255)
                                                     aTCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATggg                                                                                            <  1:146506/149‑1 (MQ=255)
                                                       ccccTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATgggg                                                                                           >  8:45341/1‑148 (MQ=255)
                                                        cccTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCTCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTGTTCGCTGTGATGGGGc                                                                                          >  4:209925/1‑148 (MQ=255)
                                                        accTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGc                                                                                          <  5:318127/147‑1 (MQ=255)
                                                          cTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTgg                                                                                                              >  4:220391/1‑126 (MQ=255)
                                                          cTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTgg                                                                                                              <  3:220391/126‑1 (MQ=255)
                                                              gaACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGcc                                                                                     >  2:241428/1‑147 (MQ=255)
                                                                     ccTAACACGCAGATTTCGGCGTTGGCCAGGGCGCCCGTGGACTTGGACGAGTGGAAATATCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTg                                                                            <  4:234411/148‑1 (MQ=255)
                                                                     aCTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTg                                                                            <  6:253280/149‑1 (MQ=255)
                                                                               aGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATc                                                                  <  7:45341/149‑1 (MQ=255)
                                                                                 atatCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCgg                                                                <  8:66421/149‑1 (MQ=255)
                                                                                   atCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCgg                                                                >  1:321393/1‑147 (MQ=255)
                                                                                      gCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCACGTAGTGCAGGGCGATCGgcagca                                                           >  6:246181/1‑149 (MQ=255)
                                                                                                 ggTCCCCCGGGAACTGGTACCCGTGTAAATACCTGATGTACGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGGCTTGGCTGTTATGGGGCAAGCCCAGTTAGTGCAGGGCTATCGCCAGCAGTTCGGGGCCa                                                <  2:86498/149‑1 (MQ=255)
                                                                                                  gTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCAt                                               <  6:232575/149‑1 (MQ=255)
                                                                                                     gCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCgg                                                     >  4:41155/1‑140 (MQ=255)
                                                                                                     gCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCgg                                                     <  3:41155/140‑1 (MQ=255)
                                                                                                                  gACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCATTGTCCAGCACCGCGAc                               >  3:62622/1‑149 (MQ=255)
                                                                                                                        tGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCATTGTCCAGCACCGCGACGCca                           >  2:311402/1‑147 (MQ=255)
                                                                                                                          gAAATTGCTTATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCATTGTCCa                                         <  2:289807/131‑1 (MQ=255)
                                                                                                                          gAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCATTGTCCAGCACCGCGACGCCACAGc                       <  7:14744/149‑1 (MQ=255)
                                                                                                                          gAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCATTGTCCAGCACCGCGACGCCACAGc                       >  5:268714/1‑149 (MQ=255)
                                                                                                                            aaTAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCATTGTCCAGCACCGCGACGCCACAGCtt                     >  5:14802/1‑149 (MQ=255)
                                                                                                                             cctgCTGATGAAAGTGCGGCCTTACATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCATTGTCCAGCACCGCGACGCCACAGCttt                    >  5:74829/4‑149 (MQ=255)
                                                                                                                                gCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCATTGTCCAGCACCGCGACGCCACAGCtttt                   >  6:153667/1‑147 (MQ=255)
                                                                                                                                gCTGATGAAAGTGCGGCCTTACATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCATTCTCCAGCACAGCGACGCCACAGCtttt                   >  1:125985/1‑147 (MQ=255)
                                                                                                                                 cTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGcc                                                                                     <  6:146358/80‑1 (MQ=255)
                                                                                                                                 cTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGcc                                                                                     <  6:141597/80‑1 (MQ=255)
                                                                                                                                 cTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGcc                                                                                     >  5:146358/1‑80 (MQ=255)
                                                                                                                                 cTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGcc                                                                                     >  5:141597/1‑80 (MQ=255)
                                                                                                                                   gATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCATTGTCCAGCACCGCGACGCCACAGCTTTTCAGCt              >  2:124936/1‑149 (MQ=255)
                                                                                                                                               cACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCATTGTCCAGCACCGCGACGCCACAGCTTTTCAGCTTGCTCATGTACt  >  7:89298/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CCCACCTTCACGCCATTGTTCGCCTCGTCGGCGCGGTTCTTCGCCTGCAGCACATCCCCTGAGAACCCCACTACCACGCAGATATCGCCGTTGGCCAGGTCGCCCGTGTACTTGGACGAGTGGAAATAGCTGATGTAAGGCCGCACTTTCATCAGCAGGTCCTTGGCCTTCTCGTAGTCCTTGGGGTCTTGGCTGTGATGGGGCAAGCCCAGGTAGTGCAGGGCGATCGGCAGCAGTTCGGGGCCATTGTCCAGCACCGCGACGCCACAGCTTTTCAGCTTGCTCATGTACT  >  NC_002947/1013622‑1013913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑