Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,038,222 C→A 7.4% P391P (CCG→CCT)  PP_0897 ← class 1 fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,038,2220CA 7.4% 212.3 / 5.2 67P391P (CCG→CCT) PP_0897class 1 fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base C (30/32);  new base A (3/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

CCGCCAACGGCCATCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGCA  >  NC_002947/1038076‑1038370
                                                                                                                                                  |                                                                                                                                                    
ccgccAACGGCCATCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCgg                                                                                                                                                    >  2:81322/1‑149 (MQ=255)
     aaCGGCCATCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCgg                                                                                                                                               <  6:94685/149‑1 (MQ=255)
             tCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCAGGCTGGGcc                                                                                                                                        >  7:304910/1‑148 (MQ=255)
                   acacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTgg                                                                                                                                           >  1:237125/1‑139 (MQ=255)
                   acacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTgg                                                                                                                                           <  2:237125/139‑1 (MQ=255)
                    cacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACtt                                                                                                                                >  2:43349/1‑149 (MQ=255)
                    cacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTAGGCCCAGCCGGCCCGACCACtg                                                                                                                                <  8:144673/149‑2 (MQ=255)
                    cacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTAGGCCCAGCCGGCCCGACCACtg                                                                                                                                >  7:144673/1‑148 (MQ=255)
                    cacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTAGGCCCAGCCGGCCCGACCACt                                                                                                                                 <  4:164552/148‑1 (MQ=255)
                     acGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTc                                                                                                                               <  7:287448/149‑1 (MQ=255)
                      cGGCCTTGGTGGCCTTGATCGCCGCGATGGCGGTCGGGCCCCGCGCGGACTTGCCAATCATGCCCAGCAGGCCAGGCTGCTCAAGGCTCTGGCGAGTGAACTTGTCCATACGGGTAGCAGGGGTCGGGCCGGCTGGGCCTACCACTTCg                                                                                                                              <  7:117575/149‑1 (MQ=255)
                       ggCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGt                                                                                                                             >  6:217011/1‑149 (MQ=255)
                       ggCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGt                                                                                                                             >  5:121204/1‑149 (MQ=255)
                        gCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTc                                                                                                                            <  4:115232/149‑1 (MQ=255)
                         ccTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTca                                                                                                                           >  8:90287/1‑149 (MQ=255)
                                 tCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCgg                                                                                                                   <  5:217011/149‑1 (MQ=255)
                                 tCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCgg                                                                                                                   >  2:53825/1‑149 (MQ=255)
                                 tCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCgg                                                                                                                   >  8:165422/1‑149 (MQ=255)
                                 tCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCgg                                                                                                                   <  3:55803/149‑1 (MQ=255)
                                    ttGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCgg                                                                                                                   >  1:156647/1‑146 (MQ=255)
                                    ttGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCgg                                                                                                                   <  2:156647/146‑1 (MQ=255)
                                       aTCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGa                                                                                                              <  6:121204/148‑1 (MQ=255)
                                        tCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGAcc                                                                                                            >  8:138566/1‑149 (MQ=255)
                                                      gTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACgtagta                                                                                              <  7:90287/149‑1 (MQ=255)
                                                                    ggACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCt                                                                                 <  5:168381/148‑1 (MQ=255)
                                                                      aCTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACGCACCGGGTCGACCGGGCCGACGTAGTAGa                                                                                            <  8:7365/135‑1 (MQ=255)
                                                                      aCTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGa                                                                                            >  7:7365/1‑135 (MQ=255)
                                                                      aCTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTc                                                                              <  8:65342/149‑1 (MQ=255)
                                                                       cTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCa                                                                             <  2:112207/149‑1 (MQ=255)
                                                                       cTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCa                                                                             <  6:201/149‑1 (MQ=255)
                                                                        ttGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                            >  6:79360/1‑149 (MQ=255)
                                                                        ttGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                            >  2:226695/1‑149 (MQ=255)
                                                                          gCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCCCCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                            <  1:226695/147‑1 (MQ=255)
                                                                          gCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCAACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCATTTAAGGt                                                                          >  8:44451/1‑149 (MQ=255)
                                                                             aaTCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGCCCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTa                                                                       >  5:167777/1‑149 (MQ=255)
                                                                              aTCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCTGACGTAGTAGATGAAGCGGCCTTTCAGGTCTAc                                                                      <  1:43349/149‑1 (MQ=255)
                                                                               tCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTAcc                                                                     <  7:138566/149‑1 (MQ=255)
                                                                                   gCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTcac                                                                                                                          <  4:37329/92‑1 (MQ=255)
                                                                                   gCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTcac                                                                                                                          >  3:37329/1‑92 (MQ=255)
                                                                                            gCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGc                                                        >  4:222587/1‑149 (MQ=255)
                                                                                            gCCAGTCTGCTCAAGGATCTAGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGc                                                        >  6:183381/1‑149 (MQ=255)
                                                                                               aGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCAc                                                     <  7:44451/149‑1 (MQ=255)
                                                                                                     cTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGAAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGa                                               <  1:81322/149‑1 (MQ=255)
                                                                                                      tCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAg                                              >  2:277501/1‑149 (MQ=255)
                                                                                                        aaGGCTCGGGCGAGTTAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCA‑CCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCAt                                           <  8:304910/149‑1 (MQ=255)
                                                                                                        aaGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTc                                                                              >  8:199783/1‑115 (MQ=255)
                                                                                                        aaGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTc                                                                              <  7:199783/115‑1 (MQ=255)
                                                                                                           gATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCAttt                                         <  5:79360/149‑1 (MQ=255)
                                                                                                                 gCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCaa                                      <  1:277501/146‑1 (MQ=255)
                                                                                                                 gCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCATTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCa                                   >  6:46092/1‑149 (MQ=255)
                                                                                                                   gAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATg                                 >  8:114333/1‑149 (MQ=255)
                                                                                                                      tGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATgcgc                              >  4:172654/1‑149 (MQ=255)
                                                                                                                        aaCTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAAc                                     <  7:128032/140‑1 (MQ=255)
                                                                                                                        aaCTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAAc                                     >  8:128032/1‑140 (MQ=255)
                                                                                                                        aaCTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCtt                            <  7:165422/149‑1 (MQ=255)
                                                                                                                        aaCTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCtt                            <  7:102314/149‑1 (MQ=255)
                                                                                                                         aCTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCtt                            <  1:53825/148‑1 (MQ=255)
                                                                                                                         aCTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTtg                           <  3:172654/149‑1 (MQ=255)
                                                                                                                          cTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGAGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTtgt                          >  1:47968/1‑149 (MQ=255)
                                                                                                                           ttGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTtgtg                         >  1:13675/1‑149 (MQ=255)
                                                                                                                             gTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTgcg                       >  4:310078/1‑149 (MQ=255)
                                                                                                                                 aTACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCgg                    >  8:285369/1‑148 (MQ=255)
                                                                                                                                  tACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCa                  >  5:178758/1‑149 (MQ=255)
                                                                                                                                      tgacgaaGTGGTAGGCCCAGCCGGCCCGACCACTTTGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCAc              >  5:49673/7‑149 (MQ=255)
                                                                                                                                      ggTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCAc              <  7:114333/149‑1 (MQ=255)
                                                                                                                                        tAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACgg            <  3:222587/149‑1 (MQ=255)
                                                                                                                                           aaGTGGTAGGCCCAGCCGGCCCGACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCg         >  8:214434/2‑149 (MQ=255)
                                                                                                                                                  cGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGCa  >  1:311431/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCGCCAACGGCCATCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGCA  >  NC_002947/1038076‑1038370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑