Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,038,234 G→C 7.6% G387G (GGC→GGG)  PP_0897 ← class 1 fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,038,2340GC 7.6% 203.4 / 7.1 66G387G (GGC→GGG) PP_0897class 1 fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base G (30/31);  new base C (3/2);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01

TCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGCATCTTG  >  NC_002947/1038089‑1038375
                                                                                                                                                 |                                                                                                                                             
tCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCAGGCTGGGcc                                                                                                                                             >  7:304910/1‑148 (MQ=255)
       cacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACtt                                                                                                                                     >  2:43349/1‑149 (MQ=255)
       cacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTAGGCCCAGCCGGCCCGACCACtg                                                                                                                                     <  8:144673/149‑2 (MQ=255)
       cacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTAGGCCCAGCCGGCCCGACCACtg                                                                                                                                     >  7:144673/1‑148 (MQ=255)
       cacGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTAGGCCCAGCCGGCCCGACCACt                                                                                                                                      <  4:164552/148‑1 (MQ=255)
        acGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTc                                                                                                                                    <  7:287448/149‑1 (MQ=255)
         cGGCCTTGGTGGCCTTGATCGCCGCGATGGCGGTCGGGCCCCGCGCGGACTTGCCAATCATGCCCAGCAGGCCAGGCTGCTCAAGGCTCTGGCGAGTGAACTTGTCCATACGGGTAGCAGGGGTCGGGCCGGCTGGGCCTACCACTTCg                                                                                                                                   <  7:117575/149‑1 (MQ=255)
          ggCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGt                                                                                                                                  >  6:217011/1‑149 (MQ=255)
          ggCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGt                                                                                                                                  >  5:121204/1‑149 (MQ=255)
           gCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTc                                                                                                                                 <  4:115232/149‑1 (MQ=255)
            ccTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTca                                                                                                                                >  8:90287/1‑149 (MQ=255)
                    tCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCgg                                                                                                                        >  8:165422/1‑149 (MQ=255)
                    tCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCgg                                                                                                                        <  5:217011/149‑1 (MQ=255)
                    tCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCgg                                                                                                                        >  2:53825/1‑149 (MQ=255)
                    tCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCgg                                                                                                                        <  3:55803/149‑1 (MQ=255)
                       ttGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCgg                                                                                                                        >  1:156647/1‑146 (MQ=255)
                       ttGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCgg                                                                                                                        <  2:156647/146‑1 (MQ=255)
                          aTCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGa                                                                                                                   <  6:121204/148‑1 (MQ=255)
                           tCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGAcc                                                                                                                 >  8:138566/1‑149 (MQ=255)
                                         gTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACgtagta                                                                                                   <  7:90287/149‑1 (MQ=255)
                                                       ggACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCt                                                                                      <  5:168381/148‑1 (MQ=255)
                                                         aCTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACGCACCGGGTCGACCGGGCCGACGTAGTAGa                                                                                                 <  8:7365/135‑1 (MQ=255)
                                                         aCTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGa                                                                                                 >  7:7365/1‑135 (MQ=255)
                                                         aCTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTc                                                                                   <  8:65342/149‑1 (MQ=255)
                                                          cTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCa                                                                                  <  2:112207/149‑1 (MQ=255)
                                                          cTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCa                                                                                  <  6:201/149‑1 (MQ=255)
                                                           ttGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                                 >  2:226695/1‑149 (MQ=255)
                                                           ttGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                                 >  6:79360/1‑149 (MQ=255)
                                                             gCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCCCCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAg                                                                                 <  1:226695/147‑1 (MQ=255)
                                                             gCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCAACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCATTTAAGGt                                                                               >  8:44451/1‑149 (MQ=255)
                                                                aaTCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGCCCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTa                                                                            >  5:167777/1‑149 (MQ=255)
                                                                 aTCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCTGACGTAGTAGATGAAGCGGCCTTTCAGGTCTAc                                                                           <  1:43349/149‑1 (MQ=255)
                                                                  tCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTAcc                                                                          <  7:138566/149‑1 (MQ=255)
                                                                      gCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTcac                                                                                                                               <  4:37329/92‑1 (MQ=255)
                                                                      gCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTcac                                                                                                                               >  3:37329/1‑92 (MQ=255)
                                                                               gCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGc                                                             >  4:222587/1‑149 (MQ=255)
                                                                               gCCAGTCTGCTCAAGGATCTAGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGc                                                             >  6:183381/1‑149 (MQ=255)
                                                                                  aGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCAc                                                          <  7:44451/149‑1 (MQ=255)
                                                                                        cTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGAAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGa                                                    <  1:81322/149‑1 (MQ=255)
                                                                                         tCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAg                                                   >  2:277501/1‑149 (MQ=255)
                                                                                           aaGGCTCGGGCGAGTTAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCA‑CCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCAt                                                <  8:304910/149‑1 (MQ=255)
                                                                                           aaGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTc                                                                                   >  8:199783/1‑115 (MQ=255)
                                                                                           aaGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTc                                                                                   <  7:199783/115‑1 (MQ=255)
                                                                                              gATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCAttt                                              <  5:79360/149‑1 (MQ=255)
                                                                                                    gCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCaa                                           <  1:277501/146‑1 (MQ=255)
                                                                                                    gCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCATTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCa                                        >  6:46092/1‑149 (MQ=255)
                                                                                                      gAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATg                                      >  8:114333/1‑149 (MQ=255)
                                                                                                         tGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATgcgc                                   >  4:172654/1‑149 (MQ=255)
                                                                                                           aaCTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAAc                                          <  7:128032/140‑1 (MQ=255)
                                                                                                           aaCTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAAc                                          >  8:128032/1‑140 (MQ=255)
                                                                                                           aaCTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCtt                                 <  7:102314/149‑1 (MQ=255)
                                                                                                           aaCTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCtt                                 <  7:165422/149‑1 (MQ=255)
                                                                                                            aCTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCtt                                 <  1:53825/148‑1 (MQ=255)
                                                                                                            aCTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTtg                                <  3:172654/149‑1 (MQ=255)
                                                                                                             cTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGAGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTtgt                               >  1:47968/1‑149 (MQ=255)
                                                                                                              ttGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTtgtg                              >  1:13675/1‑149 (MQ=255)
                                                                                                                gTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTgcg                            >  4:310078/1‑149 (MQ=255)
                                                                                                                    aTACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCgg                         >  8:285369/1‑148 (MQ=255)
                                                                                                                     tACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCa                       >  5:178758/1‑149 (MQ=255)
                                                                                                                         tgacgaaGTGGTAGGCCCAGCCGGCCCGACCACTTTGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCAc                   >  5:49673/7‑149 (MQ=255)
                                                                                                                         ggTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCAc                   <  7:114333/149‑1 (MQ=255)
                                                                                                                           tAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACgg                 <  3:222587/149‑1 (MQ=255)
                                                                                                                              aaGTGGTAGGCCCAGCCGGCCCGACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCg              >  8:214434/2‑149 (MQ=255)
                                                                                                                                     cGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGCa       >  1:311431/1‑149 (MQ=255)
                                                                                                                                      gggCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCACCTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGCAt      >  3:202503/1‑149 (MQ=255)
                                                                                                                                          cGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGCATCTTg  <  7:285369/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TCAGGTACACGGCCTTGTTGTCCTTGATCGCCTCGATGGCGGTCGGGCCACGCTCGGACTTGCCAATCATGCCCAGCAGGCCAGTCTGCTCAAGGATCTGGCGAGTGAACTTGTCCATACGGGTAGCAGTGGTCGGGCCGGCTGGGCCTACCACTTCGTCACCCACCGGGTCGACCGGGCCGACGTAGTAGATGAAGCGGCCTTTCAGGTCTACCGGCAACTCTTCGCCACGGTTGAGCATTTCAACCATGCGCTTGTGCGCGGCATCACGGCCGGTCAGCATCTTG  >  NC_002947/1038089‑1038375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑