Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,104,167 T→A 6.6% M309K (ATG→AAG)  murA → UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,104,1670TA 6.6% 183.5 / 2.9 61M309K (ATG→AAG) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (31/26);  new base A (2/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01

CATCCTTGAAGCCGTGCTTGAAAAGCTCAAGGAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAGGCAACACCGCCATCG  >  NC_002947/1104021‑1104310
                                                                                                                                                  |                                                                                                                                               
cATCCTTGAAGCCGTGCTTGAAAAGCTCAAGGAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGc                                                                                                                                               >  8:205905/1‑149 (MQ=255)
cATCCTTGAAGCCGTGCTTGAAAAGCTCAAGGAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGACGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGc                                                                                                                                               >  8:205903/1‑149 (MQ=255)
  tCCTTGAAGCCGTGCTTGAAAAGCTCAAGGAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAg                                                                                                                                             <  1:187618/149‑1 (MQ=255)
       gAAGCCGTGCTTGAAAAGCTCAAGGAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGgcg                                                                                                                                          >  2:153194/1‑147 (MQ=255)
             gTGCTTGAAAAGCTCAAGGAAGCGGGTGCCGACATCAACACCTGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCAGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCAt                                                                                                                                  <  5:238454/149‑1 (MQ=255)
             gTGCTTGAAAAGCTCAAGGAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCa                                                                                                                                   >  7:236255/1‑148 (MQ=255)
              tGCTTGAAAAGCTCAAGGAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCAtc                                                                                                                                 >  8:15339/1‑149 (MQ=255)
                       aGCTCAAGGAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAAt                                                                                                                        <  8:167018/149‑1 (MQ=255)
                        gCTCAAGGAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATg                                                                                                                       >  5:272678/1‑149 (MQ=255)
                            aaGGAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCag                                                                                                                   >  4:51648/1‑148 (MQ=255)
                            aaGGAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCa                                                                                                                    <  6:128844/148‑1 (MQ=255)
                              ggAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTg                                                                                                                 >  2:152040/1‑149 (MQ=255)
                              ggAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTg                                                                                                                 <  8:236255/149‑1 (MQ=255)
                                aaGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGcc                                                                                                               >  4:98001/1‑149 (MQ=255)
                                aaGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGcc                                                                                                               >  4:93485/1‑149 (MQ=255)
                                     ggTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGaa                                                                                                            <  2:17899/147‑1 (MQ=255)
                                     ggTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGaa                                                                                                            >  1:17899/1‑147 (MQ=255)
                                     ggTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAgg                                                                                                          >  6:57388/1‑149 (MQ=255)
                                      gTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATTCCATTGCCGAAgg                                                                                                          >  2:5346/1‑148 (MQ=255)
                                          cGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTg                                                                                                     <  5:57388/149‑1 (MQ=255)
                                           gACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTgg                                                                                                    >  8:5739/1‑149 (MQ=255)
                                            aCATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGATGAATGACATTGCCGAAGGCACTg                                                                                                     >  3:224304/1‑147 (MQ=255)
                                               tCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACAGGcgcg                                                                                                >  7:128329/1‑149 (MQ=255)
                                                 aaCACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGt                                                                                              >  8:299610/1‑149 (MQ=255)
                                                    acCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGAt                                                                                           <  7:5739/149‑1 (MQ=255)
                                                     ccGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACAAGCAGGCGCAGTTc                                                                                                                                    <  8:42923/107‑1 (MQ=255)
                                                     ccGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACAAGCAGGCGCAGTTc                                                                                                                                    >  7:42923/1‑107 (MQ=255)
                                                        gCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATc                                                                                          >  4:149095/1‑146 (MQ=255)
                                                           aaGTCTGGATGGAACTGGACATGCTTGGCAAGCGGCCGTAAGCCGTCATGCTGCGTACTGCCCCGTATCCGGCGTTGCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGGCTTTGCCGAAGGCACTGGCGCGGTGATCGAGAcc                                                                                    <  4:214910/149‑1 (MQ=255)
                                                                  gATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTc                                                                              >  4:241355/1‑148 (MQ=255)
                                                                   aTCGAACTGGACATGCATGGCAAGCGGCCGAAAGTCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACAAGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCg                                                                             >  6:229887/1‑148 (MQ=255)
                                                                       aaCTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAAc                                                                        >  4:316420/1‑149 (MQ=255)
                                                                        aCTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAAcc                                                                       >  6:51981/1‑149 (MQ=255)
                                                                              catgcatgGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTca                                                                 <  7:15339/149‑1 (MQ=255)
                                                                                 gcatgGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTcatg                                                               >  3:307404/1‑148 (MQ=255)
                                                                                   atgGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTcatgcat                                                            <  1:152040/149‑1 (MQ=255)
                                                                                   atgGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTcatgcat                                                            <  3:241355/149‑1 (MQ=255)
                                                                                       cAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGtt                                                         >  8:134146/1‑148 (MQ=255)
                                                                                        aaGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTa                                                       >  2:63839/1‑149 (MQ=255)
                                                                                           cGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGaa                                                                           >  7:1876/1‑126 (MQ=255)
                                                                                           cGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGaa                                                                           <  8:1876/126‑1 (MQ=255)
                                                                                             gCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGaaa                                                  <  1:5346/149‑1 (MQ=255)
                                                                                               cGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATg                                                <  7:134146/149‑1 (MQ=255)
                                                                                                gAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCACGCATGTTTACGAAATGc                                               <  7:299610/149‑1 (MQ=255)
                                                                                                   aGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCTGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCAcc                                            <  7:205905/149‑1 (MQ=255)
                                                                                                   aGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCTGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCACGCATGTTTACGAAATGCAcc                                            <  7:205903/149‑1 (MQ=255)
                                                                                                      cGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCa                                         >  8:223183/1‑149 (MQ=255)
                                                                                                             cTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTAc                                                      >  5:279925/1‑129 (MQ=255)
                                                                                                             cTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTAc                                                      <  6:279925/129‑1 (MQ=255)
                                                                                                                    cTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGAt                            <  3:98001/148‑1 (MQ=255)
                                                                                                                    cTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGAt                            <  3:93485/148‑1 (MQ=255)
                                                                                                                        cccGTATCCGGCGTTCCCGACCGACAAGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGATCCAgg                       <  5:229887/149‑1 (MQ=255)
                                                                                                                              tCCGGCGTTCCCGACCTACATGCAGGCGCAGTTCATCTCGCTGAATGACATTGCCGAAGGCACTGGCGCGGTTATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAg                 <  4:224304/149‑1 (MQ=255)
                                                                                                                              tCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAg                 <  1:63839/149‑1 (MQ=255)
                                                                                                                              tCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAg                 <  8:105933/149‑1 (MQ=255)
                                                                                                                                cGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGTACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAgg                >  7:260166/1‑148 (MQ=255)
                                                                                                                                cGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAgg                >  1:324902/1‑148 (MQ=255)
                                                                                                                                  gCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAGGCaa             <  3:149095/149‑1 (MQ=255)
                                                                                                                                   cGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAGGCAac            <  7:223183/149‑1 (MQ=255)
                                                                                                                                         cgaccgacATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAGGCAACACCGc       >  3:277390/1‑148 (MQ=255)
                                                                                                                                             cgacATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAGGCAACACCGCCATCg  >  8:141591/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CATCCTTGAAGCCGTGCTTGAAAAGCTCAAGGAAGCGGGTGCCGACATCAACACCGGCGAAGACTGGATCGAACTGGACATGCATGGCAAGCGGCCGAAAGCCGTCAACCTGCGTACTGCCCCGTATCCGGCGTTCCCGACCGACATGCAGGCGCAGTTCATCTCGCTGAATGCCATTGCCGAAGGCACTGGCGCGGTGATCGAGACCATCTTCGAAAACCGCTTCATGCATGTTTACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAGGCAACACCGCCATCG  >  NC_002947/1104021‑1104310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑