Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,220,941 T→A 10.6% E122V (GAA→GTA) ‡ dctD‑II ← C4‑dicarboxylate transport transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,220,9410TA10.6% 124.3 / 6.0 47E122V (GAA→GTA) ‡dctD‑IIC4‑dicarboxylate transport transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (25/17);  new base A (2/3);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

ACGTTGGCTGAGGTGTCGGCGACGTTGGCGATCAGTTCGCGCAAGTGCTCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGG  >  NC_002947/1220794‑1221081
                                                                                                                                                   |                                                                                                                                            
aCGTTGGCTGAGGTGTCGGCGACGTTGGCGATCAGTTCGCGCAAGTGCTCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTc                                                                                                                                             <  7:117753/149‑1 (MQ=255)
         gAGGTGTCGGCGACGTTGGCGATCAGTTCGCGCAAGTGCTCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGcgcgcg                                                                                                                                    >  8:194066/1‑149 (MQ=255)
         gAGGTGTCGGCGACGTTGGCGATCAGTTCGCGCAAGTGCTCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCAGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGGAACAgcgcggg                                                                                                                                    <  2:246983/149‑3 (MQ=255)
           ggTGTCGGCGACGTTGGCGATCAGTTCGCGCAAGTGCTCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCgcggc                                                                                                                                  <  8:2961/149‑1 (MQ=255)
            gtgtCGGCGACGTTGGCGATCAGTTCGCGCAAGTGCTCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCgcggcg                                                                                                                                 >  7:187824/1‑149 (MQ=255)
            gtgtCGGCGACGTTGGCGATCAGTTCGCGCAAGTGCTCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGGAACAgcgcgggct                                                                                                                                  <  6:170389/148‑5 (MQ=255)
                  gCGACGTTGGCGATCAGTTCGCGCAAGTGCTCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGGAACAgcgcgggc                                                                                                                                   >  7:248117/1‑138 (MQ=255)
                  gCGACGTTGGCGATCAGTTCGCGCAAGTGCTCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGGAACAgcgcgggc                                                                                                                                   <  8:248117/141‑4 (MQ=255)
                             gATCAGTTCGCGCAAGTGCTCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGgc                                                                                                                >  7:28574/1‑149 (MQ=255)
                                  gTTCGCGCAAGTGCTCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACAACTTCCCGTGACAGCCCGCGCTGTTAAAGCGCGCGGCGTACCACGTCGACCAGGCGCTCg                                                                                                           >  7:58607/1‑149 (MQ=255)
                                      gcgcAAGTGCTCCATGGGCGGGGAGCGACCTATGATGCGTCCCTCCTGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCGCGCGCTGTTGCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCgggg                                                                                                        <  8:257708/148‑1 (MQ=255)
                                      gcgcAAGTGCTCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCgggg                                                                                                        <  5:57115/148‑1 (MQ=255)
                                      gcgcAAGTGCTCCATGGCCGGGGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCgggg                                                                                                        <  8:261678/148‑1 (MQ=255)
                                                tCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGttt                                                                                             >  7:154648/1‑149 (MQ=255)
                                                  cATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGtttt                                                                                            <  7:224142/148‑1 (MQ=255)
                                                  cATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGatttt                                                                                           >  2:224702/1‑149 (MQ=255)
                                                    tGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTc                                                                                          >  4:258562/1‑148 (MQ=255)
                                                      gCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGGAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCAt                                                                                       <  3:80959/149‑1 (MQ=255)
                                                      gCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCAt                                                                                       <  2:255390/149‑1 (MQ=255)
                                                            gAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTc                                                                                 >  3:28809/1‑149 (MQ=255)
                                                                          gCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTAc                                                                   >  5:139371/1‑149 (MQ=255)
                                                                           cGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACg                                                                  >  3:181548/1‑149 (MQ=255)
                                                                                        tgctCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGAGGCGTACCACGTCGACCAGGCGCTCGGGGCAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGACTACg                                                     >  3:23756/1‑149 (MQ=255)
                                                                                             tGTTCGGCCAGCTGGCGGCGCAGGGCCCCCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCAt                                                >  7:248589/1‑149 (MQ=255)
                                                                                             tGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCAt                                                >  4:139966/1‑149 (MQ=255)
                                                                                             tGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCAt                                                >  7:169318/1‑149 (MQ=255)
                                                                                                 cGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTGCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTc                                             >  4:154781/1‑148 (MQ=255)
                                                                                                 cGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTc                                                                                 >  8:38003/1‑112 (MQ=255)
                                                                                                 cGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTc                                             >  2:412/1‑148 (MQ=255)
                                                                                                  ggCCAGCGGGCGGCGCAGGGCCAACACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGa                                           <  8:58607/149‑1 (MQ=255)
                                                                                                     cAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGt                                                                                  <  2:27461/107‑1 (MQ=255)
                                                                                                     cAGCTGGCGGCGCAGGGCCACCACTTCGCGAGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGt                                                                                  >  1:27461/1‑107 (MQ=255)
                                                                                                          ggcggcGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGATTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCa                                   <  1:224702/149‑1 (MQ=255)
                                                                                                              gcgcAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGc                               <  4:193657/149‑1 (MQ=255)
                                                                                                                gcAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGc                               >  7:41695/1‑147 (MQ=255)
                                                                                                                      ccaccaCTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATc                       <  5:43909/149‑1 (MQ=255)
                                                                                                                      ccaccaCTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGCCCATGTCCATATAGCCATGTCCAGTGAt                        >  1:100404/1‑148 (MQ=255)
                                                                                                                       caccacTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCa                      <  4:28809/149‑1 (MQ=255)
                                                                                                                        accacTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAg                     >  4:317056/1‑149 (MQ=255)
                                                                                                                          cacTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGca                   >  2:40614/1‑149 (MQ=255)
                                                                                                                           acTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGcac                  <  3:258562/149‑1 (MQ=255)
                                                                                                                             ttCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCCCGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCAGGTCGATATCGCCATGGCCAGTGATCAGcacca                >  3:44736/1‑149 (MQ=255)
                                                                                                                                   tGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCa          <  1:40614/149‑1 (MQ=255)
                                                                                                                                       gccgcgcgCTGGAACAGCGCG‑GGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATAGCCAGTGATCAGCACCACCGGCAGGCTg     >  3:154936/5‑149 (MQ=255)
                                                                                                                                        gCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTg     >  4:188069/1‑149 (MQ=255)
                                                                                                                                          ccGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCg   <  8:187824/149‑1 (MQ=255)
                                                                                                                                           cgcgCTGTTCCAGCGCGCGGCGTACCACGTCGCCCAGGCGCGCGGGGGCGCACGGTGTTTCCATGAAGTCGTAGGCGCCGTTCCGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCCCCACCGGCCGGCTGCgg  >  3:59736/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
ACGTTGGCTGAGGTGTCGGCGACGTTGGCGATCAGTTCGCGCAAGTGCTCCATGGCCGGTGAGCGACCAATGATGCGTCCCTCCAGGCTGCTCTGTTCGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGG  >  NC_002947/1220794‑1221081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑