Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,254,662 C→A 6.7% W280L (TGG→TTG)  metG ← methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,254,6620CA 6.7% 188.4 / 2.8 59W280L (TGG→TTG) metGmethionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (29/26);  new base A (2/2);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CGTCAGGTAGCCGTGCACGTTGACCGCGGTCGGCTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCGAAGTACGGCGCGTCGCGGGAGA  >  NC_002947/1254523‑1254809
                                                                                                                                           |                                                                                                                                                   
cGTCAGGTAGCCGTGCACGTTGACCGCGGTCGGCTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCTGGCACGAAGGctc                                                                                                                                            <  5:224795/149‑3 (MQ=255)
         gCCGTGCACGTTGACCGCGGTCGGCTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCa                                                                                                                                   <  3:116561/149‑1 (MQ=255)
                  gTTGACCGCGGTCGGCTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGggc                                                                                                                          >  5:144759/1‑149 (MQ=255)
                   ttGACCGCGGTCGACTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGggcg                                                                                                                         >  6:238437/1‑149 (MQ=255)
                      aCCGCGGTCGGCTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGggcggc                                                                                                                       <  7:289547/148‑1 (MQ=255)
                      aCCGCGGTCGGCTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGgcggcg                                                                                                                      <  3:11324/149‑1 (MQ=255)
                          cgGTCGGCTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTgcg                                                                                                                  >  1:324835/1‑149 (MQ=255)
                          cgGTCGGCTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTg                                                                                                                    >  3:140081/1‑147 (MQ=255)
                          cgGTCGGCTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTg                                                                                                                    <  4:140081/147‑1 (MQ=255)
                             tCGGCTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTgcg                                                                                                                  >  8:85338/1‑146 (MQ=255)
                                 cTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGt                                                                                                           >  2:170566/1‑149 (MQ=255)
                                 cTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGt                                                                                                           >  3:27007/1‑149 (MQ=255)
                                      ggAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCtt                                                                                                       <  1:207666/148‑1 (MQ=255)
                                      ggAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGCTCTTg                                                                                                      <  7:287705/149‑1 (MQ=255)
                                          gCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGCAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGc                                                                                                  >  1:303162/1‑149 (MQ=255)
                                            cGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCTGGCACGAAGGct                                                                                                                                             >  6:114064/1‑103 (MQ=255)
                                            cGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCTGGCACGAAGGct                                                                                                                                             <  5:114064/104‑2 (MQ=255)
                                             ggCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTgg                                                                                               <  2:324835/149‑1 (MQ=255)
                                             ggCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTgg                                                                                               >  8:221972/1‑149 (MQ=255)
                                              gcgcCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGggcg                                                                                                                         >  3:305795/1‑122 (MQ=255)
                                              gcgcCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGggcg                                                                                                                         <  4:305795/122‑1 (MQ=255)
                                                gcCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCa                                                                                            <  1:288930/149‑1 (MQ=255)
                                                       aGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGcc                                                                                     >  8:164241/1‑149 (MQ=255)
                                                         cATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGa                                                                                   >  8:278707/1‑149 (MQ=255)
                                                             gCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGCTTCTTGAAGCTGGCCATGTAGCCGATTg                                                                                <  1:185637/148‑1 (MQ=255)
                                                              cAGGCCAGAACAGGGCGTGGAATTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGt                                                                              >  7:47691/1‑149 (MQ=255)
                                                              cAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGt                                                                              <  1:36272/149‑1 (MQ=255)
                                                              cAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGt                                                                              <  4:27007/149‑1 (MQ=255)
                                                              cAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGt                                                                              <  7:221972/149‑1 (MQ=255)
                                                                        cAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGcc                                                                    <  2:121007/149‑1 (MQ=255)
                                                                        cAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGc                                                                     >  5:198889/1‑148 (MQ=255)
                                                                                ggAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTTGAGCCTTCGTGAAAGAAGGCGTCGAATTCAAGCTCCGTGCGGCTTGAGCAGAGGTTATTGAATCTGGcc                                                                                             >  7:289789/1‑116 (MQ=255)
                                                                                       gACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTAt                                                      <  1:170566/148‑1 (MQ=255)
                                                                                         cGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTg                                                   <  7:85338/149‑1 (MQ=255)
                                                                                                cTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCt                                            >  6:290853/1‑149 (MQ=255)
                                                                                                     cGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAATTCCAGCTCCGGGCGTCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGaa                                                          <  5:111900/130‑1 (MQ=255)
                                                                                                     cGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTTGACATGTAGCCGATTGGTGCATCCAGCCATAAGTAGaa                                                          >  6:111900/1‑130 (MQ=255)
                                                                                                     cGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCGCGccc                                       <  2:72251/149‑1 (MQ=255)
                                                                                                        tGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGAtt                                                                                 >  1:241024/1‑104 (MQ=255)
                                                                                                        tGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGAtt                                                                                 <  2:241024/104‑1 (MQ=255)
                                                                                                           aGTGGTACAGCTCGGGCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCGTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGCAGTATTTGCCAGGCTGGCGCGGGAtc                                 >  4:189237/1‑149 (MQ=255)
                                                                                                           aGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGAtc                                 <  2:303162/149‑1 (MQ=255)
                                                                                                             tGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGAtctc                               >  2:238489/1‑149 (MQ=255)
                                                                                                                tACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCg                              >  2:49785/1‑147 (MQ=255)
                                                                                                                 aCAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAg                           >  5:2049/1‑149 (MQ=255)
                                                                                                                    gCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGCAGCGGGCCATGTAGCCTATTGCTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCg                        <  3:189237/149‑1 (MQ=255)
                                                                                                                    gCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCg                        <  6:144759/149‑1 (MQ=255)
                                                                                                                        ggCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGAAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCGaa                      <  5:238437/147‑1 (MQ=255)
                                                                                                                          ccTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCGAAGTAc                  <  8:47691/149‑1 (MQ=255)
                                                                                                                           cTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCGAAGTACg                 >  7:79379/1‑149 (MQ=255)
                                                                                                                                 gCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCGAAGTACGGCGCGt           >  5:198900/1‑149 (MQ=255)
                                                                                                                                   cTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTG‑CAGGCTCGCCCGGGATCTCGAAGCCGAAGTACGGCGCGTcgc        >  7:204261/1‑149 (MQ=255)
                                                                                                                                    ttCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGAtc                                 >  3:207177/1‑124 (MQ=255)
                                                                                                                                    ttCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGAtc                                 <  4:207177/124‑1 (MQ=255)
                                                                                                                                    ttCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCGAAGTACGGCGCGTcgc        >  5:119857/1‑149 (MQ=255)
                                                                                                                                        tGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCGAAGTACGGCGCGTCGCgg      >  7:108634/1‑147 (MQ=255)
                                                                                                                                        tGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCGAAGTACGGCGCGTCGCgg      >  2:233017/1‑147 (MQ=255)
                                                                                                                                        tGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCGAAGTACGGCGCGTCGCGGga    <  6:119857/149‑1 (MQ=255)
                                                                                                                                         gCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCGAAGTACGGCGCGTCGCgg      >  3:118833/1‑146 (MQ=255)
                                                                                                                                          ccAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCGAAGTACGGCGCGTCGCGGgaga  <  5:88783/149‑1 (MQ=255)
                                                                                                                                          ccAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCACGCCCGAGATCTCGAAGCCGAAGTACGGCGCGTCGCGGgag   <  7:278707/148‑1 (MQ=255)
                                                                                                                                           cAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCGAAGTACGGCGCGTCGCGGgaga  >  5:174451/1‑148 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
CGTCAGGTAGCCGTGCACGTTGACCGCGGTCGGCTTGCGGAAGCCGGCGCCTTCGAGCATGGCAGGCCAGAACAGGGCGTGGAAGTTGACGATGTCCTTGCCGATGAAGTGGTACAGCTCGGCCTTGGAGCCTTCGTGCCAGAAGGCGTCGAAGTCCAGCTCCGGGCGGCGTGCGCAGAGGTTCTTGAAGCTGGCCATGTAGCCGATTGGTGCATCCAGCCATACGTAGAAGTATTTGCCAGGCTCGCCCGGGATCTCGAAGCCGAAGTACGGCGCGTCGCGGGAGA  >  NC_002947/1254523‑1254809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑