Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,255,335 A→G 8.5% S56P (TCG→CCG)  metG ← methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,255,3350AG 8.5% 128.7 / 2.6 47S56P (TCG→CCG) metGmethionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (20/23);  new base G (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CTCGCGGTTCTCGTCGCTGTGGGTGGAGTGGAAATTGTCGAAGTCCACCAGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGGATCGATCCATTGGCATAGGGCAGGGCGCTGGTGACGAGA  >  NC_002947/1255189‑1255480
                                                                                                                                                  |                                                                                                                                                 
cTCGCGGTTCTCGTCGCTGTGGGTGGAGTGGAAATTGTCGAAGTCCACCAGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAAc                                                                                                                                                 <  3:150407/149‑1 (MQ=255)
            gtcgCTGTGGGTGGAGTGGAAATTGTCGAAGTCCACCAGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCa                                                                                                                                      >  4:9469/1‑148 (MQ=255)
            gtcgCTGTGGGTGGAGTGGAAATTGTCGAAGTCCACCAGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCAt                                                                                                                                     <  3:9469/149‑1 (MQ=255)
                cTGTGGGTGGAGTGGAAATTGTCGAAGTCCACCAGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGgc                                                                                                                                 <  5:303050/149‑1 (MQ=255)
                 tgtgGGTGGAGTGGAAATTGTCGAAGTCCACCAGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGgcg                                                                                                                                >  3:89118/1‑149 (MQ=255)
                  gtgggtggAGTGGAAATTGTCGAAGTCCACCAGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGgcgc                                                                                                                               <  3:74299/149‑1 (MQ=255)
                       tggagtggaAATTGTCGAAGTCCACCAGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACa                                                                                                                          >  3:3853/1‑149 (MQ=255)
                         gagtggaAATTGTCGAAGTCCACCAGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACAt                                                                                                                         <  4:89118/148‑1 (MQ=255)
                          agtggaAATTGTCGAAGTCCACCAGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCTGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAg                                                                                                                       <  1:176446/149‑1 (MQ=255)
                                       gAAGTCCACCAGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGc                                                                                                          <  5:46163/149‑1 (MQ=255)
                                            ccaccaGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCcgcg                                                                                                      >  2:179218/1‑148 (MQ=255)
                                              accaGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTgg                                                                                                                                            >  3:7514/1‑108 (MQ=255)
                                              accaGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTgg                                                                                                                                            <  4:7514/108‑1 (MQ=255)
                                                   gAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCt                                                                                              <  8:131873/149‑1 (MQ=255)
                                                    aaGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTg                                                                                             <  7:291993/149‑1 (MQ=255)
                                                      gTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGGACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTgg                                                                                            >  3:155781/1‑148 (MQ=255)
                                                      gTCGGCGAAATCGCTGATGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGGACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTgg                                                                                            >  3:150781/1‑148 (MQ=255)
                                                              aaTCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCAcc                                                                                   >  2:44521/1‑149 (MQ=255)
                                                                tCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGGACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCca                                                                                 <  4:155781/149‑1 (MQ=255)
                                                                tCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGGACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCca                                                                                 <  4:150781/149‑1 (MQ=255)
                                                                   ctgctgTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTCGCCGCGCAGCTTCTGGAAGCGCACCCACAt                                                                              >  8:186738/1‑149 (MQ=255)
                                                                        gtgCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCcac                                                                                >  8:186759/1‑142 (MQ=255)
                                                                        gtgCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCcac                                                                                <  7:186759/142‑1 (MQ=255)
                                                                           cTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTc                                                                       <  8:96578/148‑1 (MQ=255)
                                                                              ggCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCATCTGGAAGCGCACCCACATGTCCGTCTGGa                                                                   <  1:29054/149‑1 (MQ=255)
                                                                               gCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGAt                                                                  <  1:179218/149‑1 (MQ=255)
                                                                                      cGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCt                                                                                              <  1:321668/114‑1 (MQ=255)
                                                                                      cGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCt                                                                                              >  2:321668/1‑114 (MQ=255)
                                                                                          ggCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGc                                                        <  7:77415/148‑1 (MQ=255)
                                                                                           gCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCa                                                       >  6:110508/1‑148 (MQ=255)
                                                                                             gATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCatat                                                    >  3:29216/1‑149 (MQ=255)
                                                                                               tCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATgg                                                  >  6:100085/1‑149 (MQ=255)
                                                                                                  gCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCg                                               <  5:110508/149‑1 (MQ=255)
                                                                                                  gCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATCTCCGTCTGGATGTACTCCAGCATATGGcc                                                >  6:269306/1‑148 (MQ=255)
                                                                                                        ccGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATg                                          >  1:164234/1‑148 (MQ=255)
                                                                                                          ggggTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGgat                                       <  1:44521/149‑1 (MQ=255)
                                                                                                             gTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGgatcga                                    <  5:269306/149‑1 (MQ=255)
                                                                                                                    cttccttTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGGATCGATCCATTg                             >  1:122511/1‑149 (MQ=255)
                                                                                                                     ttccttTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGGATCGATCCATTgg                            >  8:159055/1‑149 (MQ=255)
                                                                                                                         ttttCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGGATCGATCCATTGGCa                          >  5:201227/1‑147 (MQ=255)
                                                                                                                         ttttCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGGATCGATCCATTGGCa                          >  3:162218/1‑147 (MQ=255)
                                                                                                                                cgcgcAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGGATCGATCCATTGGCATagggcagg                 <  4:162218/149‑1 (MQ=255)
                                                                                                                                           atgGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGGATCGATCCATTGGCATAGGGCAGGGCGCTGGTGAc      <  7:159055/149‑1 (MQ=255)
                                                                                                                                           atgGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGGATCGATCCATTGGCATAGGGCAGGGCGCTGGTGAc      <  2:164234/149‑1 (MQ=255)
                                                                                                                                               ccGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGGATCGATCCATTGGCATAGGGCAGGGCGCTGGTGACgaga  <  3:266818/149‑1 (MQ=255)
                                                                                                                                               ccGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGGATCGATCCATTGGCATAGGGCAGGGCGCTGGTGACgag   >  2:57152/1‑148 (MQ=255)
                                                                                                                                               ccGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGGATCGATCCATTGGCATAGGGCAGGGCGCTGGTGACgag   >  3:273972/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTCGCGGTTCTCGTCGCTGTGGGTGGAGTGGAAATTGTCGAAGTCCACCAGGAAGTCGGCGAAATCGCTGCTGTGCTCGGCCTGGACGTTGGCGATCAGCTGTTCCGGGGTGATGCCTTCCTTTTCGGCGCGCAGCATGATGGCCGAACCGTGGGCGTCATCGGCGCAGACATAGATGCACTGGTTGCCGCGCAGCTTCTGGAAGCGCACCCACATGTCCGTCTGGATGTACTCCAGCATATGGCCGAGATGGATCGATCCATTGGCATAGGGCAGGGCGCTGGTGACGAGA  >  NC_002947/1255189‑1255480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑