Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,292,213	A→T	5.2%	H335L (CAC→CTC)	estC →	carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,292,213	0	A	T	5.2%	232.4 / 2.4	77	H335L (CAC→CTC)	estC	carboxylesterase
Reads supporting (aligned to +/- strand):  ref base A (33/40);  new base T (2/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01

TCCGAACTGCTCGATGAGCTGACCCGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTACGCGTCCTTGCCAGTTGCCTTTGATCGGG  >  NC_002947/1292065‑1292360                                                                                                                                                     |                                                                                                                                                    tCCGAACTGCTCGATGAGCTGACCCGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCa                                                                                                                                                     >  7:168960/1‑149 (MQ=255)  ccGAACTGCTCGATGAGCTGACCAGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCAc                                                                                                                                                    >  6:287533/1‑149 (MQ=255)      aCTGCTCGATGAGCTGACCCGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTg                                                                                                                                                >  5:206248/1‑149 (MQ=255)      aCTGCTCGATGAGCTGACCCGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTg                                                                                                                                                <  6:266109/149‑1 (MQ=255)             gATGAGCTGACCCGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCgg                                                                                                                                         >  5:76050/1‑149 (MQ=255)                 aGCTGACCCGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCggtggt                                                                                                                                     <  8:74858/149‑1 (MQ=255)                   cTGACCCGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTtc                                                                                                                                   <  6:163536/149‑1 (MQ=255)                      aCCCGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTtcg                                                                                                                                  <  5:309671/147‑1 (MQ=255)                        ccGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGtt                                                                                                                                    >  1:12454/1‑143 (MQ=255)                        ccGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGtt                                                                                                                                    <  2:12454/143‑1 (MQ=255)                        ccGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTtcggtcg                                                                                                                              >  1:172288/1‑149 (MQ=255)                        ccGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTtcggtcg                                                                                                                              >  6:185734/1‑149 (MQ=255)                        ccGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTtcggtcg                                                                                                                              >  3:33800/1‑149 (MQ=255)                         cgcgAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTtcggtcgg                                                                                                                             >  1:17463/1‑149 (MQ=255)                         cgcgAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTtcggtcgg                                                                                                                             >  7:266460/1‑149 (MQ=255)                            gAGCATAGCCTTGGCCAGGACCGTACCTTCCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGttt                                                                                                                          <  5:287533/149‑1 (MQ=255)                             aGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGtttt                                                                                                                         <  6:316539/149‑1 (MQ=255)                               cATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGc                                                                                                                       <  4:67429/149‑1 (MQ=255)                               cATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGc                                                                                                                       <  2:74872/149‑1 (MQ=255)                                aTAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGcc                                                                                                                      >  4:7209/1‑149 (MQ=255)                                        ggCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCg                                                                                                                <  6:76050/147‑1 (MQ=255)                                         gCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTg                                                                                                                                                >  7:270845/1‑114 (MQ=255)                                         gCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTg                                                                                                                                                <  8:270845/114‑1 (MQ=255)                                                ccGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTAGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGAtg                                                                                                      >  6:24000/1‑149 (MQ=255)                                                  gTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACAAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGAtgtg                                                                                                    <  2:97229/149‑1 (MQ=255)                                                   tACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTgg                                                                                                   <  2:192970/149‑1 (MQ=255)                                                     ccTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGc                                                                                                  <  6:293912/148‑1 (MQ=255)                                                         gCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTc                                                                                              <  7:229467/148‑1 (MQ=255)                                                           tCACCCAGACCCGTTTCGGCCTGGGCTGGATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGt                                                                                           >  1:20919/1‑149 (MQ=255)                                                           tCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGt                                                                                           >  5:120077/1‑149 (MQ=255)                                                           tCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGt                                                                                           >  1:15768/1‑149 (MQ=255)                                                             aCCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGtt                                                                                          <  7:251837/148‑1 (MQ=255)                                                               ccAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGTCGACCCGGAGTACGATGTGGCCTTCGGTTTtg                                                                                       >  8:212663/1‑149 (MQ=255)                                                               ccAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTtg                                                                                       <  7:88967/149‑1 (MQ=255)                                                               ccAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTtg                                                                                       <  6:206248/149‑1 (MQ=255)                                                                cAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTtgt                                                                                      <  8:266460/149‑1 (MQ=255)                                                                cAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTtgt                                                                                      <  2:17463/149‑1 (MQ=255)                                                                 aGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCTCCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGa                                                                                                              <  7:83934/124‑1 (MQ=255)                                                                 aGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCTCCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGa                                                                                                              >  8:83934/1‑124 (MQ=255)                                                                 aGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCTCCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGa                                                                                                              <  7:88528/124‑1 (MQ=255)                                                                 aGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCTCCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGa                                                                                                              >  8:88528/1‑124 (MQ=255)                                                                     ccGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGtt                                                                                                                                    >  4:4741/1‑98 (MQ=255)                                                                     ccGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTc                                                                                  >  4:208959/1‑148 (MQ=255)                                                                       gTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCaa                                                                                >  8:17200/1‑148 (MQ=255)                                                                             gCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGt                                                                                   <  8:218036/139‑1 (MQ=255)                                                                             gCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGt                                                                                   >  7:218036/1‑139 (MQ=255)                                                                               cTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCgg                                                                       >  2:164485/1‑149 (MQ=255)                                                                                       cctGCTCGACCAGCCCGAGGTGGCCAATGCCACCTGTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTAt                                                                <  6:4124/146‑1 (MQ=255)                                                                                       cATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGACTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTAtg                                                               >  1:82504/1‑149 (MQ=255)                                                                                        aTGCTCGACCAGCCCGATGGGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTAtgt                                                              <  8:150006/149‑1 (MQ=255)                                                                                          gCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGc                                                            >  5:101373/1‑149 (MQ=255)                                                                                           cTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTa                                                                                                           <  6:20871/101‑1 (MQ=255)                                                                                           cTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTa                                                                                                           >  5:20871/1‑101 (MQ=255)                                                                                           cTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTCTGTGc                                                            <  3:7209/148‑1 (MQ=255)                                                                                           cTCGACCAGCCCGAGGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCt                                                           <  4:33800/149‑1 (MQ=255)                                                                                              gACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCGTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTg                                                          <  5:125025/147‑1 (MQ=255)                                                                                              gACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGTCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTg                                                          <  2:172288/147‑1 (MQ=255)                                                                                              gACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGAt                                                        <  2:20919/149‑1 (MQ=255)                                                                                              gACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGAt                                                        <  2:15768/149‑1 (MQ=255)                                                                                                 cAGCCAGATGTTGCCAATGCCACCTTTGGTCTCGGCGCTCGCGCCTTTGGCAACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGGTGATgta                                                     <  8:168960/149‑3 (MQ=255)                                                                                                     ccGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCtt                                                                  <  6:192634/132‑1 (MQ=255)                                                                                                     ccGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTATTGCCGACCCGGAGTACGATGTGGCCTTCGGATTTGTGGTCAATACCCTCGGCCCGTATGTGCTGATGGACCCa                                                 >  7:298011/1‑149 (MQ=255)                                                                                                      cGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCAc                                                <  7:17200/149‑1 (MQ=255)                                                                                                             gCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGccc                                          <  5:185734/148‑1 (MQ=255)                                                                                                             gCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGcc                                           >  2:15718/1‑147 (MQ=255)                                                                                                               cAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACACTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAg                                        >  8:213818/1‑148 (MQ=255)                                                                                                                 aTGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGaaa                                     <  1:164485/149‑1 (MQ=255)                                                                                                                        cTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTAc                              <  1:140940/149‑1 (MQ=255)                                                                                                                              tctcGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTACGCGTcc                        >  2:163729/1‑149 (MQ=255)                                                                                                                                tcGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTACGCGTCCtt                      >  1:306403/1‑149 (MQ=255)                                                                                                                                tcGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTACGCGTCCtt                      >  6:97354/1‑149 (MQ=255)                                                                                                                                tcGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTACGCGTCCtt                      >  8:205237/1‑149 (MQ=255)                                                                                                                                  ggCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTACGCGTCCTTGc                    <  2:82504/149‑1 (MQ=255)                                                                                                                                    cgcCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGTCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTACGCGTCCTTGCCa                  <  7:212663/149‑1 (MQ=255)                                                                                                                                         gcgcCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTACGCGTCCTTGCCAGTTGc             <  2:306403/149‑1 (MQ=255)                                                                                                                                          cgcCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTACGCGTCCTTGCCAGTTGcc            <  7:205237/149‑1 (MQ=255)                                                                                                                                              tttGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTACGCGTCGTTGCCAGTTGCCttt         >  6:160475/1‑148 (MQ=255)                                                                                                                                                  gCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTACGCGTCCTTGCCAGTTGCCTTTGATCg    >  5:250415/1‑149 (MQ=255)                                                                                                                                                     aCCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTACGCGTCCTTGCCAGTTGCCTTTGATCggg  >  2:59477/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TCCGAACTGCTCGATGAGCTGACCCGCGAGCATAGCCTTGGCCAGGACCGTACCTTGCTCACCCAGACCCGTTTCGGCCTGGGCTGCATGCTCGACCAGCCCGATGTGGCCAATGCCACCTTTGGTCTCGGCGCCCGCGCCTTTGGCCACCCTGGTGCCGGTGGTTCGGTCGGTTTTGCCGACCCGGAGTACGATGTGGCCTTCGGTTTTGTGGTCAATACCCTCGGCCCTTATGTGCTGATGGACCCACGCGCCCAGAAACTGGTACGCGTCCTTGCCAGTTGCCTTTGATCGGG  >  NC_002947/1292065‑1292360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑