Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,330,563	T→G	6.9%	intergenic (+94/‑213)	PP_5SE → / → PP_1157	5S ribosomal RNA/acetolactate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,330,563	0	T	G	6.9%	209.1 / 3.9	69	intergenic (+94/‑213)	PP_5SE/PP_1157	5S ribosomal RNA/acetolactate synthase
Reads supporting (aligned to +/- strand):  ref base T (30/34);  new base G (2/3);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTGGC  >  NC_002947/1330416‑1330709                                                                                                                                                    |                                                                                                                                                    tCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCtt                                                                                                                                                    >  3:93735/1‑149 (MQ=255)   gCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTc                                                                                                                                                   <  4:253169/148‑1 (MQ=255)           tAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTTGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgtgc                                                                                                                                           <  6:32746/148‑2 (MQ=255)              tgtgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTcgc                                                                                                                                       <  5:330639/149‑1 (MQ=255)                tgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCg‑g                                                                                                                                    <  3:101776/149‑1 (MQ=255)                tgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCg‑g                                                                                                                                    <  3:126165/149‑1 (MQ=255)                 ggggTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCg‑gg                                                                                                                                   >  8:174057/1‑149 (MQ=255)                  gggTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGt                                                                                                                                  <  2:156187/149‑1 (MQ=255)                         cccATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCg                                                                                                                           >  7:135501/1‑149 (MQ=255)                          ccATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTcgc                                                                                                                                       >  7:36612/1‑137 (MQ=255)                          ccATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTcgc                                                                                                                                       <  8:36612/137‑1 (MQ=255)                          ccATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGc                                                                                                                          >  2:291125/1‑149 (MQ=255)                           cATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCt                                                                                                                         >  6:286387/1‑149 (MQ=255)                            aTGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTc                                                                                                                        <  5:286387/149‑1 (MQ=255)                            aTGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGCGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTc                                                                                                                        <  6:8087/149‑1 (MQ=255)                                    gTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACagag                                                                                                                <  3:191820/149‑1 (MQ=255)                                    gTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACagag                                                                                                                <  3:92967/149‑1 (MQ=255)                                     tAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACagag                                                                                                                >  4:311940/1‑148 (MQ=255)                                        gTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgtgcca                                                                                                                                         <  2:243378/121‑4 (MQ=255)                                        gTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgtgcca                                                                                                                                         >  1:243378/1‑118 (MQ=255)                                          cATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGt                                                                                                                                  >  8:248081/1‑125 (MQ=255)                                          cATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGt                                                                                                                                  <  7:248081/125‑1 (MQ=255)                                           aTCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTg                                                                                                         <  4:93735/149‑1 (MQ=255)                                               tCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCg                                                                                                       >  5:263006/1‑147 (MQ=255)                                                cAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGCCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAg                                                                                                    >  7:15916/1‑149 (MQ=255)                                                             gCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATg                                                                                       <  4:45156/149‑1 (MQ=255)                                                               aaaaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGc                                                                                     >  7:64462/1‑149 (MQ=255)                                                               aaaaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCATTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTAGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGc                                                                                     >  1:222703/1‑149 (MQ=255)                                                                aaaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGATAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGcc                                                                                    <  5:173068/149‑1 (MQ=255)                                                                 aaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGAATGGTGCGAAGCCAGGCTAGAATGGCCt                                                                                   >  7:280423/1‑149 (MQ=255)                                                                    cccTATCTGCGCACGCAGGTAGGGGCTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGATTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCaa                                                                                >  1:77796/1‑149 (MQ=255)                                                                    cccTACCTGCGTGCGCAGATAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCaa                                                                                >  5:293449/1‑149 (MQ=255)                                                                        aTCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGc                                                                                                        >  2:163841/1‑121 (MQ=255)                                                                        aTCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGc                                                                                                        <  1:163841/121‑1 (MQ=255)                                                                        aTCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTAGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCGTCAACg                                                                              >  5:295918/1‑147 (MQ=255)                                                                         tCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACgcag                                                                           >  3:238199/1‑149 (MQ=255)                                                                         tCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACgca                                                                            <  7:174057/148‑1 (MQ=255)                                                                                acgcaGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGaa                                                                                                     <  5:45872/116‑1 (MQ=255)                                                                                acgcaGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGaa                                                                                                     >  6:45872/1‑116 (MQ=255)                                                                                    aGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGa                                                                <  1:291125/149‑1 (MQ=255)                                                                                      gTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACt                                                              >  8:41722/1‑149 (MQ=255)                                                                                      gTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgtgcc                                                                                                                                          <  4:148897/74‑3 (MQ=25)                                                                                      gTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgtgcc                                                                                                                                          >  3:148897/1‑72 (MQ=25)                                                                                                  tCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGt                                                                                                          <  6:169644/93‑1 (MQ=255)                                                                                                    tttGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCAcccccc                                                >  1:148769/1‑149 (MQ=255)                                                                                                     ttGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCAcccccc                                                >  4:178104/1‑148 (MQ=255)                                                                                                      tgtgGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCAcccccc                                                <  8:64462/147‑1 (MQ=255)                                                                                                        tgGGCAAAAAATTGTACCTGCTAGAGAGCGGATATGGCACCGGCTTCTCCGGTGTTCGCG‑TGTAATCCAGCTACTACAGAGGATGGTTCGAAGCCAGGCTAGAATGGCATCAACGCAGCATTAACGTGACTTTCCCGCACACCCCCTaa                                            <  7:207957/149‑2 (MQ=255)                                                                                                        tgGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTAc                                            >  4:166918/1‑149 (MQ=255)                                                                                                             aaaaaaTTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCaa                                       <  3:178104/149‑1 (MQ=255)                                                                                                              aaaaaTTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑AGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAg                                      >  1:247916/1‑149 (MQ=255)                                                                                                               aaaaTTGGACCTGCTCGAGAGCGGATATGGCCCCGGCTTCGCCGGTGTCGAAGTGGTAAACCCGCTCCTACTGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAg                                      <  1:105897/149‑1 (MQ=255)                                                                                                               aaaaTTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTTGCCGGTGTTCGCG‑GGTAAACCCGCTCGTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGAGTTTCCCGCACCCCCCCTACGTCAAGa                                     <  2:222703/149‑1 (MQ=255)                                                                                                               aaaaTTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACTCCCCCTACGTCaa                                       >  7:201398/1‑147 (MQ=255)                                                                                                               aaaaTTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGa                                     >  2:206632/1‑149 (MQ=255)                                                                                                                     ggACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑TGTAAACCCGCTCCTACAGAGAATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTg                                <  8:280423/148‑1 (MQ=255)                                                                                                                     ggACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTg                                <  3:166918/148‑1 (MQ=255)                                                                                                                         cTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCg                           >  1:223987/1‑149 (MQ=255)                                                                                                                          tGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCgg                          >  2:124847/1‑149 (MQ=255)                                                                                                                           gCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCggg                         >  3:191275/1‑149 (MQ=255)                                                                                                                            cTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCgg                          <  2:247916/147‑1 (MQ=255)                                                                                                                               gagaGCGGAGATGGCACCGGCTTAGCCGGTGTTCGCG‑GGTAAACCCTCTCCTACAGAGGATGCTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCAGCACCACCCCTACGTCAAGACTTTGGTTCGGGGCAc                     >  6:257953/1‑149 (MQ=38)                                                                                                                                      gagaTGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCt              >  8:261023/1‑149 (MQ=255)                                                                                                                                       agaTGGCACCGGATTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCATCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTc             <  2:77796/149‑1 (MQ=255)                                                                                                                                         aTGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTcc            <  4:191275/148‑1 (MQ=255)                                                                                                                                             cACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGAc       <  2:148769/149‑1 (MQ=255)                                                                                                                                             cACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGAc       <  1:124847/149‑1 (MQ=255)                                                                                                                                               ccGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCt     <  3:311940/149‑1 (MQ=255)                                                                                                                                                 ggCTTCGCCGGTGTGCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTgg   <  1:242672/149‑1 (MQ=255)                                                                                                                                                  gCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTGGc  <  6:293449/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TCGCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTGGC  >  NC_002947/1330416‑1330709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑