Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,330,565	C→A	11.5%	intergenic (+96/‑211)	PP_5SE → / → PP_1157	5S ribosomal RNA/acetolactate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,330,565	0	C	A	11.5%	194.1 / 7.4	68	intergenic (+96/‑211)	PP_5SE/PP_1157	5S ribosomal RNA/acetolactate synthase
Reads supporting (aligned to +/- strand):  ref base C (27/32);  new base A (5/3);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTGGCGAT  >  NC_002947/1330418‑1330712                                                                                                                                                    |                                                                                                                                                     gCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTc                                                                                                                                                      <  4:253169/148‑1 (MQ=255)         tAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTTGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgtgc                                                                                                                                              <  6:32746/148‑2 (MQ=255)            tgtgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTcgc                                                                                                                                          <  5:330639/149‑1 (MQ=255)              tgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCg‑g                                                                                                                                       <  3:126165/149‑1 (MQ=255)              tgGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCg‑g                                                                                                                                       <  3:101776/149‑1 (MQ=255)               ggggTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCg‑gg                                                                                                                                      >  8:174057/1‑149 (MQ=255)                gggTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGt                                                                                                                                     <  2:156187/149‑1 (MQ=255)                       cccATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCg                                                                                                                              >  7:135501/1‑149 (MQ=255)                        ccATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTcgc                                                                                                                                          <  8:36612/137‑1 (MQ=255)                        ccATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTcgc                                                                                                                                          >  7:36612/1‑137 (MQ=255)                        ccATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGc                                                                                                                             >  2:291125/1‑149 (MQ=255)                         cATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCt                                                                                                                            >  6:286387/1‑149 (MQ=255)                          aTGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTc                                                                                                                           <  5:286387/149‑1 (MQ=255)                          aTGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGCGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTc                                                                                                                           <  6:8087/149‑1 (MQ=255)                                  gTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACagag                                                                                                                   <  3:92967/149‑1 (MQ=255)                                  gTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACagag                                                                                                                   <  3:191820/149‑1 (MQ=255)                                   tAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACagag                                                                                                                   >  4:311940/1‑148 (MQ=255)                                      gTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgtgcca                                                                                                                                            <  2:243378/121‑4 (MQ=255)                                      gTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgtgcca                                                                                                                                            >  1:243378/1‑118 (MQ=255)                                        cATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGt                                                                                                                                     >  8:248081/1‑125 (MQ=255)                                        cATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGt                                                                                                                                     <  7:248081/125‑1 (MQ=255)                                         aTCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTg                                                                                                            <  4:93735/149‑1 (MQ=255)                                             tCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCg                                                                                                          >  5:263006/1‑147 (MQ=255)                                              cAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGCCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAg                                                                                                       >  7:15916/1‑149 (MQ=255)                                                           gCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATg                                                                                          <  4:45156/149‑1 (MQ=255)                                                             aaaaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGc                                                                                        >  7:64462/1‑149 (MQ=255)                                                             aaaaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCATTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTAGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGc                                                                                        >  1:222703/1‑149 (MQ=255)                                                              aaaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGATAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGcc                                                                                       <  5:173068/149‑1 (MQ=255)                                                               aaCCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGAATGGTGCGAAGCCAGGCTAGAATGGCCt                                                                                      >  7:280423/1‑149 (MQ=255)                                                                  cccTATCTGCGCACGCAGGTAGGGGCTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGATTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCaa                                                                                   >  1:77796/1‑149 (MQ=255)                                                                  cccTACCTGCGTGCGCAGATAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCaa                                                                                   >  5:293449/1‑149 (MQ=255)                                                                      aTCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGc                                                                                                           >  2:163841/1‑121 (MQ=255)                                                                      aTCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGc                                                                                                           <  1:163841/121‑1 (MQ=255)                                                                      aTCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTAGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCGTCAACg                                                                                 >  5:295918/1‑147 (MQ=255)                                                                       tCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACgcag                                                                              >  3:238199/1‑149 (MQ=255)                                                                       tCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACgca                                                                               <  7:174057/148‑1 (MQ=255)                                                                              acgcaGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGaa                                                                                                        >  6:45872/1‑116 (MQ=255)                                                                              acgcaGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGaa                                                                                                        <  5:45872/116‑1 (MQ=255)                                                                                  aGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGa                                                                   <  1:291125/149‑1 (MQ=255)                                                                                    gTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACt                                                                 >  8:41722/1‑149 (MQ=255)                                                                                    gTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgtgcc                                                                                                                                             >  3:148897/1‑72 (MQ=25)                                                                                    gTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCGAAGCCGgtgcc                                                                                                                                             <  4:148897/74‑3 (MQ=25)                                                                                                tCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGt                                                                                                             <  6:169644/93‑1 (MQ=255)                                                                                                  tttGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCAcccccc                                                   >  1:148769/1‑149 (MQ=255)                                                                                                   ttGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCAcccccc                                                   >  4:178104/1‑148 (MQ=255)                                                                                                    tgtgGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCAcccccc                                                   <  8:64462/147‑1 (MQ=255)                                                                                                      tgGGCAAAAAATTGTACCTGCTAGAGAGCGGATATGGCACCGGCTTCTCCGGTGTTCGCG‑TGTAATCCAGCTACTACAGAGGATGGTTCGAAGCCAGGCTAGAATGGCATCAACGCAGCATTAACGTGACTTTCCCGCACACCCCCTaa                                               <  7:207957/149‑2 (MQ=255)                                                                                                      tgGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTAc                                               >  4:166918/1‑149 (MQ=255)                                                                                                           aaaaaaTTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCaa                                          <  3:178104/149‑1 (MQ=255)                                                                                                            aaaaaTTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑AGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAg                                         >  1:247916/1‑149 (MQ=255)                                                                                                             aaaaTTGGACCTGCTCGAGAGCGGATATGGCCCCGGCTTCGCCGGTGTCGAAGTGGTAAACCCGCTCCTACTGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAg                                         <  1:105897/149‑1 (MQ=255)                                                                                                             aaaaTTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTTGCCGGTGTTCGCG‑GGTAAACCCGCTCGTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGAGTTTCCCGCACCCCCCCTACGTCAAGa                                        <  2:222703/149‑1 (MQ=255)                                                                                                             aaaaTTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACTCCCCCTACGTCaa                                          >  7:201398/1‑147 (MQ=255)                                                                                                             aaaaTTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGa                                        >  2:206632/1‑149 (MQ=255)                                                                                                                   ggACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑TGTAAACCCGCTCCTACAGAGAATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTg                                   <  8:280423/148‑1 (MQ=255)                                                                                                                   ggACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTg                                   <  3:166918/148‑1 (MQ=255)                                                                                                                       cTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCg                              >  1:223987/1‑149 (MQ=255)                                                                                                                        tGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCgg                             >  2:124847/1‑149 (MQ=255)                                                                                                                         gCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCggg                            >  3:191275/1‑149 (MQ=255)                                                                                                                          cTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCgg                             <  2:247916/147‑1 (MQ=255)                                                                                                                             gagaGCGGAGATGGCACCGGCTTAGCCGGTGTTCGCG‑GGTAAACCCTCTCCTACAGAGGATGCTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCAGCACCACCCCTACGTCAAGACTTTGGTTCGGGGCAc                        >  6:257953/1‑149 (MQ=38)                                                                                                                                    gagaTGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCt                 >  8:261023/1‑149 (MQ=255)                                                                                                                                     agaTGGCACCGGATTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCATCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTc                <  2:77796/149‑1 (MQ=255)                                                                                                                                       aTGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTcc               <  4:191275/148‑1 (MQ=255)                                                                                                                                           cACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGAc          <  1:124847/149‑1 (MQ=255)                                                                                                                                           cACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGAc          <  2:148769/149‑1 (MQ=255)                                                                                                                                             ccGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCt        <  3:311940/149‑1 (MQ=255)                                                                                                                                               ggCTTCGCCGGTGTGCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTgg      <  1:242672/149‑1 (MQ=255)                                                                                                                                                gCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTGGc     <  6:293449/149‑1 (MQ=255)                                                                                                                                                    cGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTGGCGAt  <  8:135501/148‑1 (MQ=255)                                                                                                                                                    cGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTGGCGAt  <  4:238199/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GCCGATGGTAGTGTGGGGTTTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCATTTCGCAAAACCCCTATCTGCGCACGCAGGTAGGGGTTTTGTCTTTGTGGGCAAAAAATTGGACCTGCTCGAGAGCGGAGATGGCACCGGCTTCGCCGGTGTTCGCG‑GGTAAACCCGCTCCTACAGAGGATGGTGCGAAGCCAGGCTAGAATGGCCTCAACGCAGCATTAACGTGACTTTCCCGCACCCCCCCTACGTCAAGACTTTGGTTCGGGGCACTTTTGCTCCTGGACCTGGCGAT  >  NC_002947/1330418‑1330712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑