Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,352,384	C→A	5.7%	intergenic (‑27/+113)	PP_1178 ← / ← nrdA	hypothetical protein/ribonucleoside‑diphosphate reductase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,352,384	0	C	A	5.7%	217.3 / 2.2	70	intergenic (‑27/+113)	PP_1178/nrdA	hypothetical protein/ribonucleoside‑diphosphate reductase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (35/31);  new base A (2/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

TGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGCCTTACTGGCAGGCTTCGCAGTCAGGCTCGTCGATC  >  NC_002947/1352240‑1352530                                                                                                                                                 |                                                                                                                                                    tgtACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGtt                                                                                                                                                 <  5:142653/149‑1 (MQ=255)   tACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGGTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTc                                                                                                                                                <  5:255206/148‑1 (MQ=255)     cAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCttt                                                                                                                                             <  1:116713/149‑1 (MQ=255)     cAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCttt                                                                                                                                             >  5:258066/1‑149 (MQ=255)       gCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTa                                                                                                                                           >  2:287698/1‑149 (MQ=255)              gACAATGTGTGTCTTGGGGTGCTTGCGTGCGTGTTCCACCGAGGTGAAACGGCCGCTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCt                                                                                                                                    >  1:27335/1‑149 (MQ=255)                 aaTGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCg                                                                                                                                 >  3:63489/1‑149 (MQ=255)                   tgtgtgTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCTGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTg                                                                                                                               <  1:97052/149‑1 (MQ=255)                     tgtgtCTTGGGGTGCTTGCGTGCCTTTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGaa                                                                                                                             <  7:129008/149‑1 (MQ=255)                     tgtgtCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGa                                                                                                                              <  4:281652/148‑1 (MQ=255)                         tCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGt                                                                                                                         <  1:175946/149‑1 (MQ=255)                           ttGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTgg                                                                                                                       >  6:153874/1‑149 (MQ=255)                            tGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTggg                                                                                                                      >  3:175822/1‑149 (MQ=255)                               ggTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTAcc                                                                                                                                         >  2:222709/1‑127 (MQ=255)                                 tGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAg                                                                                                                 <  7:146561/149‑1 (MQ=255)                                   cTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAgg                                                                                                                <  2:46995/148‑1 (MQ=255)                                    ttGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAggg                                                                                                               >  8:179245/1‑148 (MQ=255)                                    ttGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAggg                                                                                                               >  6:161415/1‑148 (MQ=255)                                        gTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAgg                                                                                                          <  1:287698/149‑1 (MQ=255)                                         tGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGt                                                                                                         >  4:138073/1‑149 (MQ=255)                                           gcTGTTCCACCGAGGCGAAACGGCCGGTTGCTGCGTTGCGACCAATCTAGCGAGATCGCAGTTTTTTCCGCGCCATGGCCGTACATCCTATTTTGCGAGGGCCTGTTCGTTTACCGAGCTGCGGGAACACTCCGCAAAGGGCAAgcctt                                                                                                       <  2:134434/148‑5 (MQ=255)                                            cTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGTGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGttt                                                                                                       <  1:109842/148‑1 (MQ=255)                                            cTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGttt                                                                                                       >  2:109842/1‑148 (MQ=255)                                             tGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTtct                                                                                                     >  7:212181/1‑149 (MQ=255)                                               ttCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTATTTGAGAGGTCCTGTTATTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTct‑ct                                                                                                  >  5:144858/1‑149 (MQ=255)                                                  cACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAg                                                                                               >  2:235871/1‑149 (MQ=255)                                                   aCCGAGGTGAAACGGCCGGTTGCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAgg                                                                                              >  6:84445/1‑149 (MQ=255)                                                    ccGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGgc                                                                                             >  5:105770/1‑149 (MQ=255)                                                          tGAAACGGCCGGTTTCTGCGTTGCGACCGATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGa                                                                                        >  3:311723/1‑148 (MQ=255)                                                           gAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGAt                                                                                       >  7:107623/1‑148 (MQ=255)                                                                ggCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTAc                                                                                                                                          <  3:243648/93‑1 (MQ=255)                                                                ggCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTAc                                                                                                                                          >  4:243648/1‑93 (MQ=255)                                                                 gCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACaaaaa                                                                                <  4:63489/149‑1 (MQ=255)                                                                     gTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGcc                                                                            >  4:160492/1‑149 (MQ=255)                                                                            cGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCcgcgc                                                                        <  5:161415/146‑1 (MQ=255)                                                                                 cGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCAc                                                                 <  6:170815/148‑1 (MQ=255)                                                                                   aCCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACAACCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCg                                                              >  1:230361/1‑149 (MQ=255)                                                                                     cAATCTTGCGAGATGACAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGATCTGCGTGAACAGTGGGCAAAGTGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGACGCGCGGGTCACCCg                                                              >  4:290711/1‑147 (MQ=255)                                                                                       aTCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCAccc                                                               >  6:29034/1‑144 (MQ=255)                                                                                       aTCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCAccc                                                               <  5:29034/144‑1 (MQ=255)                                                                                           tGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGcgcg                                                                                          >  6:124215/1‑113 (MQ=255)                                                                                           tGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGcgcg                                                                                          <  5:124215/113‑1 (MQ=255)                                                                                           tGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATg                                                                              >  4:17470/1‑125 (MQ=255)                                                                                           tGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATg                                                                              <  3:17470/125‑1 (MQ=255)                                                                                              gagaTGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCg                                                   >  4:163034/1‑149 (MQ=255)                                                                                                    ccAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACgg                                              >  2:39710/1‑148 (MQ=255)                                                                                                       gTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCAtt                                          >  8:81429/1‑149 (MQ=255)                                                                                                        ttttttCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCAtt                                          >  2:280821/1‑148 (MQ=255)                                                                                                          ttttCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCa                                        <  5:153874/148‑1 (MQ=255)                                                                                                           tttCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAg                                       <  2:27335/148‑1 (MQ=255)                                                                                                             accGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGc                                     <  1:280821/147‑1 (MQ=255)                                                                                                              ccGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGCCt                                   >  6:81352/1‑149 (MQ=255)                                                                                                                gcgcCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGc                                     >  6:115394/1‑145 (MQ=255)                                                                                                                gcgcCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGc                                     <  5:115394/145‑1 (MQ=255)                                                                                                                gcgcCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGCCtt                                  >  3:289971/1‑148 (MQ=255)                                                                                                                    cATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGCCTTACTgg                             <  2:230361/149‑1 (MQ=255)                                                                                                                               atacTTTTTTGAGAGGTCATGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCTCCTGAGGCGCGGATACAAAAATGCCGCGCgg                                                                      <  1:195015/95‑1 (MQ=255)                                                                                                                               aTCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCCCCCGTAGGCCCCCCGCACCGCATTCAGGCCGTAATTGCAGGcgtgg                    >  2:315620/1‑143 (MQ=255)                                                                                                                               aTCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGCCTTACTGGCAGGCTTCGCa                  <  1:39710/149‑1 (MQ=255)                                                                                                                               aTCCTTTTTTGAGAGGTCATGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCgg                                                                      >  6:324060/1‑97 (MQ=255)                                                                                                                               aTCCTTTTTTGAGAGGTCATGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCgg                                                                      <  5:324060/97‑1 (MQ=255)                                                                                                                               aTCCTTTTTTGAGAGGTCATGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCgg                                                                      >  2:195015/1‑97 (MQ=255)                                                                                                                                  cTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGCCTTACTGGCAGGCTTCGCAGTc               <  8:107623/149‑1 (MQ=255)                                                                                                                                   ttttttGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCACACGGCATTCAGGCCTTACTGGCAGGCTTCGCAGt                >  3:80192/1‑147 (MQ=255)                                                                                                                                     ttttGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGCCTTACTGGCAGGCTTCGCAGTCa              <  4:311723/147‑1 (MQ=255)                                                                                                                                     ttttGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGCCTTACTGGCAGGCTTCGCAGTCa              <  7:81429/147‑1 (MQ=255)                                                                                                                                        tGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGCCTTACTGGCAGGCTTCGCAGTCAGGCt          <  4:289971/148‑1 (MQ=255)                                                                                                                                         gagaGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGACGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGCCTTTCTGGCAGGCTTCGCAGTCAGGCtc         <  6:144858/148‑1 (MQ=255)                                                                                                                                              gTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATAC‑AAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGCCTTACTGGCAGGCTTCGCAGTCAGGCTCGTCGATc  >  8:59803/1‑149 (MQ=255)                                                                                                                                               tCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATGCAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGCCTTACTGGCAGGCTTCGCAGTCAGGCTCGTCGATc  <  1:235871/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    TGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGCCATGGGCGTACATCCTTTTTTGAGAGGTCCTGTTCTTTTACCGAGCTGCGTGAACAGTGGGCAAAGGGCAAGGTTTCT‑CTGAGGCGCGGATACAAAAATGCCGCGCGGGGCACCCGTAGGCACCCCGCACGGCATTCAGGCCTTACTGGCAGGCTTCGCAGTCAGGCTCGTCGATC  >  NC_002947/1352240‑1352530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑