Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	147,556	G→A	12.7%	A178A (GCC→GCT)	PP_0139 ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	147,556	0	G	A	12.7%	199.3 / 15.3	79	A178A (GCC→GCT)	PP_0139	lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (36/32);  new base A (6/4);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGCTGGCCGAGTTTTCAGGGGTAGTCGCGGCGATCTTCGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTC  >  NC_002947/147408‑147703                                                                                                                                                     |                                                                                                                                                     cACTGCTGGCCGAGTTTTCAGGGGTAGTCGCGGCGATCTTCGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCg                                                                                                                                                      <  8:270800/149‑1 (MQ=255)  aCTGCTGGCCGAGTTTTCAGGGGTAGTCGCGGCGATCTTCGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCgg                                                                                                                                                     <  2:237233/149‑1 (MQ=255)   ctgctgGCCGAGTTTTCAGGGGTAGTCGCGGCGATCTTCGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGc                                                                                                                                                    <  7:287193/149‑1 (MQ=255)      ctgGCCGAGTTTTCAGGGGTAGTCGCGGCGATCTTCGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCCGGCTTCGGCCAGGCCAGCGAc                                                                                                                                                 <  2:8234/149‑1 (MQ=255)           cGAGTTTTCAGGGGTAGTCGCGGCGATCTTGGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑ctg                                                                                                                                           >  4:92242/1‑149 (MQ=255)            gAGTTTTCAGGGGTAGTCGCGGCGATCTTCGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑ctgc                                                                                                                                          <  6:9601/149‑1 (MQ=255)             aGTTTTCAGGGGTAGTCGCGGCGATCTTCGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑ctgct                                                                                                                                         <  3:200720/149‑1 (MQ=255)               ttttCAGGGGTAGTCGCGGCGATCTTCGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGa                                                                                                                                       >  3:153839/1‑149 (MQ=255)                        gTAGTCGCGGCGATCTTCGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGttt                                                                                                                              <  4:65671/149‑1 (MQ=255)                            tCGCGGCGATCTTCGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGc                                                                                                                                                    <  4:316616/124‑1 (MQ=255)                            tCGCGGCGATCTTCGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGc                                                                                                                                                    >  3:316616/1‑124 (MQ=255)                                      cTTCGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAACCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGtt                                                                                                                >  3:104963/1‑149 (MQ=255)                                          gTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGc                                                                                                             <  4:153839/148‑1 (MQ=255)                                           tCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCACCACCAGCTGGTCGCTGGCCTGGCCGAAGACGCCGACCA‑CTGCTGATACCTGTTTGTCGTCAAGCGGTTGGCg                                                                                                            >  7:117643/1‑148 (MQ=255)                                           tCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCACCACCAGCAGGTAGCTGGCCTGGACGAAGCCGGCGACCA‑CTGCTGATACCTCTTTGTCGACCATCGGTTGGCg                                                                                                            >  7:113288/1‑148 (MQ=255)                                              gCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGAtt                                                                                                        >  8:283201/1‑149 (MQ=255)                                               cTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGAttt                                                                                                       <  2:47305/149‑1 (MQ=255)                                               cTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGAttt                                                                                                       >  1:47305/1‑149 (MQ=255)                                                 aCTGGTTCTTTGCCTGCAGCTGCCGTGGTGCCTGCTTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCAACAGCTGGTCGCTGGCCTTGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCg                                                                                                     <  4:182748/149‑1 (MQ=255)                                                 aCTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTc                                                                                                      >  5:293076/1‑148 (MQ=255)                                                   tGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCg                                                                                                     >  5:201901/1‑147 (MQ=255)                                                          ttGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTg                                                                                            >  2:259491/1‑149 (MQ=255)                                                                 aaGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCTAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTc                                                                                                      >  6:38383/1‑132 (MQ=255)                                                                 aaGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCTAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTc                                                                                                      <  5:38383/132‑1 (MQ=255)                                                                 aaGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGc                                                                                       <  7:283201/147‑1 (MQ=255)                                                                   gCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAg                                                                                    >  7:72922/1‑148 (MQ=255)                                                                    cTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGAGTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAACCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGa                                                                                  <  4:104963/149‑1 (MQ=255)                                                                    cTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGa                                                                                  <  7:247564/149‑1 (MQ=255)                                                                    cTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGa                                                                                  <  2:87797/149‑1 (MQ=255)                                                                     ttCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGTTGGCCAGGAt                                                                                 >  3:160574/1‑149 (MQ=255)                                                                     ttCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGAt                                                                                 >  1:302257/1‑149 (MQ=255)                                                                          ttGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCgtt                                                                            <  6:293076/149‑1 (MQ=255)                                                                             tgCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCgttggt                                                                         >  2:194967/1‑149 (MQ=255)                                                                               ccTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCgttggtt                                                                        <  2:302257/148‑1 (MQ=255)                                                                                 tGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGaa                                                                                                   >  8:204241/1‑119 (MQ=255)                                                                                 tGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGaa                                                                                                   <  7:204241/119‑1 (MQ=255)                                                                                 tGATTGGCCTGGGTGATGGTCCAGTTAACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCg                                                                     >  8:184913/1‑149 (MQ=255)                                                                                         cTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGa                                                             >  7:100069/1‑149 (MQ=255)                                                                                         cTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGa                                                             <  3:92242/149‑1 (MQ=255)                                                                                         cTGGGGGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGa                                                             <  7:302587/149‑1 (MQ=255)                                                                                            ggTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGcc                                                                  <  2:301180/141‑1 (MQ=255)                                                                                            ggTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGcc                                                                  >  1:301180/1‑141 (MQ=255)                                                                                               gATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAagc                                                       <  1:259491/149‑1 (MQ=255)                                                                                                aTGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAgg                                                                                   >  7:253777/1‑120 (MQ=255)                                                                                                aTGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAgg                                                                                   <  8:253777/120‑1 (MQ=255)                                                                                                aTGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCgt                                                                             <  1:287059/126‑1 (MQ=255)                                                                                                aTGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCgt                                                                             >  2:287059/1‑126 (MQ=255)                                                                                                    tCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAagcgagcg                                                  >  5:75733/1‑149 (MQ=255)                                                                                                    tCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAagcgagc                                                   <  7:223798/148‑1 (MQ=255)                                                                                                    tCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAagcgagc                                                   >  8:223798/1‑148 (MQ=255)                                                                                                       aGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTc                                                >  4:107284/1‑148 (MQ=255)                                                                                                           cACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGc                                           >  5:180492/1‑149 (MQ=255)                                                                                                           cACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGc                                           >  5:129745/1‑149 (MQ=255)                                                                                                            aCGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGc                                           <  1:194967/148‑1 (MQ=255)                                                                                                                   gCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCTGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATcac                                    <  8:117643/148‑1 (MQ=255)                                                                                                                   gCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATAGCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATcac                                    <  8:113288/148‑1 (MQ=255)                                                                                                                    ccccACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATcacc                                   <  6:180492/148‑1 (MQ=255)                                                                                                                      ccaccaGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCaccacc                                >  1:220316/1‑149 (MQ=255)                                                                                                                       tcagcagtGGTCGCCGGCTTCGGCCAGGCCAGCGACCAGCTGGTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCaccacc                                >  5:3419/8‑149 (MQ=25)                                                                                                                         ccaGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTc                              >  7:44987/1‑148 (MQ=255)                                                                                                                          gcagtGGTCGCCGGCTTCGGCCAGGCCAGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCa                            >  1:78733/5‑149 (MQ=38)                                                                                                                          gcagtGGTCGCCGGCTTCGGCCAGGCCAGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCa                            >  7:198635/5‑149 (MQ=38)                                                                                                                          gcagtGGTCGCCGGCTTCGGCCAGGCCAGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCa                            >  5:301226/5‑149 (MQ=38)                                                                                                                          gcagtGGTCGCCGGCTTCGGCCAGGCCAGCGACCA‑CTGCTGATACCTGTTTGTCGACCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGTATGAGGCCCAGCACCAAGCGAGCGTCAAAGCGCATCACCACCTCCa                            >  4:219432/5‑149 (MQ=25)                                                                                                                          caGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCg                                                                     >  3:160645/1‑108 (MQ=255)                                                                                                                          caGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCg                                                                     <  4:160645/108‑1 (MQ=255)                                                                                                                            gCTGGTCGCTGGCCTGGCCGAAGTCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACt                          >  2:150327/1‑149 (MQ=255)                                                                                                                            agtGGTCGCCGGCTTCGGCCAGGCCAGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACt                          >  5:322254/3‑149 (MQ=255)                                                                                                                                gTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGccc                      >  6:152067/1‑149 (MQ=255)                                                                                                                                        cctggccGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATcac                                    >  4:245476/1‑127 (MQ=255)                                                                                                                                        cctggccGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATcac                                    <  3:245476/127‑1 (MQ=255)                                                                                                                                        cctggccGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTg              >  5:140907/1‑149 (MQ=255)                                                                                                                                         ctggccGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGc             >  6:43194/1‑149 (MQ=255)                                                                                                                                         ctggccGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTACGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGc             >  4:122337/1‑149 (MQ=255)                                                                                                                                            gccGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCg            <  6:129745/147‑1 (MQ=255)                                                                                                                                            gccGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCg            <  3:107284/147‑1 (MQ=255)                                                                                                                                            gccGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGGTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCgg           <  7:184913/148‑1 (MQ=255)                                                                                                                                             gccaggCCAGCGACCA‑CTGCTGATACCTGTTTGTCGTCGAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTa         <  3:219432/144‑1 (MQ=255)                                                                                                                                              ccaggCCAGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTAt        <  6:301226/145‑1 (MQ=255)                                                                                                                                              ccaggCCAGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTAt        <  8:198635/145‑1 (MQ=255)                                                                                                                                               gAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTAtt       <  6:201901/149‑1 (MQ=255)                                                                                                                                                     ggCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCActc  <  1:150327/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CACTGCTGGCCGAGTTTTCAGGGGTAGTCGCGGCGATCTTCGTCTGCTACTGGTTCTTTGCCTGAAGCTTCAGTTGTGCCTGATTGGCCTGGGTGATGGTCCAGTTCACGACTTGCCCCACCAGCTGGTCGCTGGCCTGGCCGAAGCCGGCGACCA‑CTGCTGATACCTGTTTGTCGTCCAGCGGTTGGCGGATTTCGAAGCGCTTGCTGGCCAGGATGCGTTGGTTGCGGCCCTGGACCAAGCGAGCGTCATAGCGGATCACCACCTCCACTGCCCCATCCGTGCGGTATTCACTC  >  NC_002947/147408‑147703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑