Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,383,254 A→T 9.1% L16Q (CTG→CAG)  PP_1204 ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,383,2540AT 9.1% 142.8 / 5.6 55L16Q (CTG→CAG) PP_1204lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (28/21);  new base T (2/3);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.18e-01

GTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTC  >  NC_002947/1383111‑1383401
                                                                                                                                               |                                                                                                                                                   
gTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTgcg                                                                                                                                                >  2:273772/1‑149 (MQ=255)
 tCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTgcgg                                                                                                                                               <  2:142535/149‑1 (MQ=255)
    cTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGAACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGgcgc                                                                                                                                            <  2:202950/149‑1 (MQ=255)
       cGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACTGTGCGGCGccc                                                                                                                                          <  6:327345/148‑1 (MQ=255)
       cGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACTGTGCGGCGcc                                                                                                                                           <  8:33141/147‑1 (MQ=255)
            gCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAgg                                                                                                                                    <  2:228457/149‑1 (MQ=255)
             cTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAggg                                                                                                                                   >  4:63852/1‑149 (MQ=255)
              tCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCCCACTGCGGCGCCCCCAAggg                                                                                                                                   >  1:322273/1‑148 (MQ=255)
                  tCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAg                                                                                                                              <  8:36066/149‑1 (MQ=255)
                  tCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAg                                                                                                                              <  6:166700/149‑1 (MQ=255)
                  tCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAg                                                                                                                              <  4:278236/149‑1 (MQ=255)
                    aCCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAgg                                                                                                                             <  6:106158/148‑1 (MQ=255)
                     ccTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACCGTGCGGGGCCCCCAAGGGTTAAGGt                                                                                                                            >  5:32694/1‑148 (MQ=255)
                     ccTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGt                                                                                                                            >  5:32693/1‑148 (MQ=255)
                               gCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCtt                                                                                                                   <  1:273772/147‑1 (MQ=255)
                               gCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGt                                                                                                                 <  5:9573/149‑1 (MQ=255)
                                     aTCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATg                                                                                                            >  4:52714/1‑148 (MQ=255)
                                             gCAGGGTATACTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGt                                                                                                   >  7:186681/1‑149 (MQ=255)
                                              cAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCAc                                                                                                       <  3:52714/144‑1 (MQ=255)
                                                      gCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGt                                                                                           >  8:229549/1‑148 (MQ=255)
                                                        tCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTaaa                                                                                                                               <  8:230052/110‑1 (MQ=255)
                                                        tCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTaaa                                                                                                                               >  7:230052/1‑110 (MQ=255)
                                                        tCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTa                                                                                         >  4:72390/1‑148 (MQ=255)
                                                                  tGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTAGCTTGTACATGATCACACCTGCATTGGTTATCAGACGCGaa                                                                              >  8:27954/1‑149 (MQ=255)
                                                                      cTCGACGCGATCCTCATGGTCGCTGCGGTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGGTATCAGCGGCGAAcgcg                                                                          <  7:27954/149‑1 (MQ=255)
                                                                       tCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCgg                                                                         >  2:279668/1‑149 (MQ=255)
                                                                         gACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTTCATGATCACTCGt                                                                                                   >  5:276244/1‑121 (MQ=255)
                                                                         gACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGt                                                                                                   <  6:276244/121‑1 (MQ=255)
                                                                            gcgcTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTg                                                                    >  6:143518/1‑149 (MQ=255)
                                                                                  ctcATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAgg                                                                >  6:296118/1‑147 (MQ=255)
                                                                                  ctcATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGaa                                                              <  3:72390/149‑1 (MQ=255)
                                                                                  ctcATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTAATTAGCTTGTTCAAGATAACTCGTGCATTGGTTTTCAGCGGCGAACGCGGGCCTGCAgg                                                                >  4:76126/1‑147 (MQ=255)
                                                                                     aTGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGTGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGt                                                           >  2:67728/1‑149 (MQ=255)
                                                                                            ctgcgctgGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTa                                                                                         >  6:288132/1‑112 (MQ=255)
                                                                                            ctgcgctgGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTa                                                                                         <  5:288132/112‑1 (MQ=255)
                                                                                                  tgGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCAtt                                               >  6:133282/1‑148 (MQ=255)
                                                                                                     gACATCTGGTGGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGc                                           <  3:76126/149‑1 (MQ=255)
                                                                                                       cATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCgg                                         <  3:63852/149‑1 (MQ=255)
                                                                                                         tCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATAGAGAGGc                                        >  6:166910/1‑148 (MQ=255)
                                                                                                          cTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTAc                                                                                                               >  1:296154/1‑76 (MQ=255)
                                                                                                             gTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGc                                   <  7:75020/149‑1 (MQ=255)
                                                                                                              ttGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAg                                                                 <  1:135992/118‑1 (MQ=255)
                                                                                                                 gcgcAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGAGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTgg                               >  3:229514/1‑149 (MQ=255)
                                                                                                                   gcAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTAGCGTGTACATAATCACTCCTGCATTGGGTATCAGCGGCGAACCCGGGCCTGCAGGAAGGTCAGCACATGATTGAGCGGCAACGCCTGGGc                             >  4:310907/1‑149 (MQ=255)
                                                                                                                         gCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTg                       >  7:242616/1‑149 (MQ=255)
                                                                                                                          cTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGc                      <  5:133282/149‑1 (MQ=255)
                                                                                                                          cTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATAAGCGGCGAACGCGGGCCTGCAGGAAGGTGAGCACTTCATTTAGCGGCAACGCCAGGGCGGCCTGc                      >  6:290687/1‑149 (MQ=255)
                                                                                                                                   aGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAGGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTcggtgcgg             >  8:279784/1‑149 (MQ=255)
                                                                                                                                    gggCGCCGCACTGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTcggtgcggt            >  4:154046/1‑149 (MQ=255)
                                                                                                                                     ggCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTcggtgcggtg           <  5:290687/149‑1 (MQ=255)
                                                                                                                                      gcgcCGCACTGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTc                          >  6:326910/1‑133 (MQ=255)
                                                                                                                                      gcgcCGCACTGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTc                          <  5:326910/133‑1 (MQ=255)
                                                                                                                                       cgccgcACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCt         >  2:321199/1‑149 (MQ=255)
                                                                                                                                          cgcACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTg       >  2:163477/1‑148 (MQ=255)
                                                                                                                                          cgcACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTg       >  5:177326/1‑148 (MQ=255)
                                                                                                                                              caGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTc  <  5:296118/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCTTGTACTC  >  NC_002947/1383111‑1383401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑