Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,384,157	T→G	10.1%	I292L (ATT→CTT)	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,384,157	0	T	G	10.1%	195.7 / 11.0	67	I292L (ATT→CTT)	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (32/28);  new base G (4/3);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

AGGTTGAGCGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCACGGTT  >  NC_002947/1384009‑1384305                                                                                                                                                     |                                                                                                                                                     aGGTTGAGCGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAAt                                                                                                                                                      <  4:194436/149‑1 (MQ=255)  ggTTGAGCGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAAt                                                                                                                                                      >  1:284328/1‑148 (MQ=255)   gTTGAGCGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAAt                                                                                                                                                      >  2:103852/1‑147 (MQ=255)         cGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGc                                                                                                                                              >  3:34938/1‑149 (MQ=255)         cGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGc                                                                                                                                              <  4:199240/149‑1 (MQ=255)           ggCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAAGCTGATTGccc                                                                                                                                            <  5:39628/149‑1 (MQ=255)                agcgagcCGGGGCCGGCGCCAATGGCGTCGCGCAGTGCGGCTTCGGTGGCCATGACCAGCGGGTCGGCATCCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTTGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTc                                                                                                                                       <  7:57738/149‑1 (MQ=255)                  cgagcCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCg                                                                                                                                      <  8:268633/148‑1 (MQ=255)                   gagcCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCgg                                                                                                                                     >  2:188329/1‑148 (MQ=255)                      ccggcgccggcgCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCAcc                                                                                                                                 <  2:284328/149‑1 (MQ=255)                        ggcgccggcgccAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACcgc                                                                                                                               <  1:35519/149‑1 (MQ=255)                            ccggcgccAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTAc                                                                                                                           <  5:181245/149‑1 (MQ=255)                              ggcgccAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGa                                                                                                                         >  8:166479/1‑149 (MQ=255)                              ggcgccAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTCGTCGCCACGGACGATCACGGCACTCAGCTTGCCCTCTTCGGCACCGCGTACg                                                                                                                          >  8:274532/1‑148 (MQ=255)                                 gacAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGAACCGCGTACGATCa                                                                                                                      <  7:274532/147‑1 (MQ=255)                                   cAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAgg                                                                                                                    <  4:77301/149‑1 (MQ=255)                                   cAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAgg                                                                                                                    >  1:187977/1‑149 (MQ=255)                                      tGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTc                                                                                                                 >  5:250350/1‑149 (MQ=255)                                           tCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGAc                                                                                                            <  7:166479/149‑1 (MQ=255)                                             gcgcAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAAGCTGATTGCCctga                                                                                                                                         <  7:288062/118‑3 (MQ=255)                                             gcgcAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAAGCTGATTGCCctga                                                                                                                                         >  8:288062/1‑116 (MQ=255)                                               gcAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTc                                                                                                        <  3:218800/149‑1 (MQ=255)                                                   ttCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCtttt                                                                                                    >  2:255442/1‑149 (MQ=255)                                                   ttCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCtttt                                                                                                    <  1:103852/149‑1 (MQ=255)                                                      ggCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGa                                                                                                 >  2:68530/1‑149 (MQ=255)                                                        cTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATc                                                                                               <  4:34938/149‑1 (MQ=255)                                                           cGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCgg                                                                                             >  7:30391/1‑148 (MQ=255)                                                             gTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAg                                                                                          >  6:77493/1‑149 (MQ=255)                                                                   aTGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTg                                                                                    <  5:244737/149‑1 (MQ=255)                                                                     gACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGt                                                                                  >  7:87469/1‑149 (MQ=255)                                                                          gCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTcc                                                                             <  5:77493/149‑1 (MQ=255)                                                                          gCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTcc                                                                             <  1:68530/149‑1 (MQ=255)                                                                          gCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTc                                                                              >  2:22150/1‑148 (MQ=255)                                                                              gTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTccacc                                                                          >  5:317595/1‑148 (MQ=255)                                                                                  gCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTg                                                                     >  4:205906/1‑149 (MQ=255)                                                                                    aaCCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGTACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTggg                                                                   >  6:95509/1‑149 (MQ=255)                                                                                    aaCCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGc                                                                                            <  2:173500/124‑1 (MQ=255)                                                                                    aaCCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGc                                                                                            >  1:173500/1‑124 (MQ=255)                                                                                     aCCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTggg                                                                   >  4:96025/1‑148 (MQ=255)                                                                                     aCCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTggg                                                                   >  2:53384/1‑148 (MQ=255)                                                                                      ccTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTc                                                                              <  5:284250/136‑1 (MQ=255)                                                                                      ccTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTc                                                                              >  6:284250/1‑136 (MQ=255)                                                                                       cTGTTCCAGCTTGGTGGCCATGATTTCGTTCAGATCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGACTTTTCGATCGGCAGGCCGTGGTTTTCAACCAACAGGGCaa                                                                >  2:41032/1‑149 (MQ=255)                                                                                          ttCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATg                                                              <  8:87469/148‑1 (MQ=255)                                                                                            ccAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTc                                                           >  1:32376/1‑149 (MQ=255)                                                                                               gCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTg                                                        <  1:255442/149‑1 (MQ=255)                                                                                                     tggCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTc                                                  <  2:187977/149‑1 (MQ=255)                                                                                                     tggCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCCTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTc                                                  >  4:175860/1‑149 (MQ=255)                                                                                                       tgcTTGATTTGGTTCAGTTCGTGCTCGCGACGGACGATATTGGCAAGAAGGTTGCCCTCTTCGGCACCGCGTTCGATCAGGGTCTTGACGTTCTTTTGGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGTCATTCg                                                 <  8:125987/146‑1 (MQ=255)                                                                                                        ccTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCggg                                               <  6:317595/149‑1 (MQ=255)                                                                                                            gATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGgtgt                                           >  8:239235/1‑149 (MQ=255)                                                                                                             aTTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGgtgt                                           <  3:205906/148‑1 (MQ=255)                                                                                                             aTTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCCTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGgtgt                                           <  3:175860/148‑1 (MQ=255)                                                                                                                  gTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATg                                                              <  8:98962/124‑1 (MQ=255)                                                                                                                  gTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATg                                                              >  7:98962/1‑124 (MQ=255)                                                                                                                   ttCAGTTCGTGGTCGCCACGGACGATCAGGCCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAg                                    >  1:26076/1‑149 (MQ=255)                                                                                                                      aGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGgcg                                 <  3:96025/149‑1 (MQ=255)                                                                                                                       gTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTc                                                                              >  7:252935/1‑103 (MQ=255)                                                                                                                       gTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTc                                                                              <  8:252935/103‑1 (MQ=255)                                                                                                                        ttCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGgcg                                 >  7:192889/1‑147 (MQ=255)                                                                                                                             ggTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGctcc                          >  7:11496/1‑149 (MQ=255)                                                                                                                               tCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCGGctcctc                        >  3:128147/1‑149 (MQ=255)                                                                                                                                        aCGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGgtgt                                           >  7:172287/1‑121 (MQ=255)                                                                                                                                        aCGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGgtgt                                           <  8:172287/121‑1 (MQ=255)                                                                                                                                          gaagaGGGCAAGCTGATTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAg             >  2:249476/5‑149 (MQ=255)                                                                                                                                           aagaGGGCAAGCTGATTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAgg            >  1:317859/4‑149 (MQ=255)                                                                                                                                           aagaGGGCAAGCTGATTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAgg            >  5:126831/4‑149 (MQ=255)                                                                                                                                                ggCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCa       >  3:309489/1‑149 (MQ=255)                                                                                                                                                   aaTCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTg                                                        <  6:190436/97‑1 (MQ=255)                                                                                                                                                   aaTCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTg                                                        >  5:190436/1‑97 (MQ=255)                                                                                                                                                     tCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCACGGtt  <  1:22150/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     AGGTTGAGCGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCACGGTT  >  NC_002947/1384009‑1384305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑