Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,384,159	A→T	8.8%	L291Q (CTG→CAG)	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,384,159	0	A	T	8.8%	194.4 / 7.7	68	L291Q (CTG→CAG)	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (32/30);  new base T (4/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-01

CGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCACGGTTTC  >  NC_002947/1384017‑1384307                                                                                                                                               |                                                                                                                                                     cGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGc                                                                                                                                                <  4:199240/149‑1 (MQ=255) cGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGc                                                                                                                                                >  3:34938/1‑149 (MQ=255)   ggCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAAGCTGATTGccc                                                                                                                                              <  5:39628/149‑1 (MQ=255)        agcgagcCGGGGCCGGCGCCAATGGCGTCGCGCAGTGCGGCTTCGGTGGCCATGACCAGCGGGTCGGCATCCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTTGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTc                                                                                                                                         <  7:57738/149‑1 (MQ=255)          cgagcCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCg                                                                                                                                        <  8:268633/148‑1 (MQ=255)           gagcCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCgg                                                                                                                                       >  2:188329/1‑148 (MQ=255)              ccggcgccggcgCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCAcc                                                                                                                                   <  2:284328/149‑1 (MQ=255)                ggcgccggcgccAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACcgc                                                                                                                                 <  1:35519/149‑1 (MQ=255)                    ccggcgccAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTAc                                                                                                                             <  5:181245/149‑1 (MQ=255)                      ggcgccAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGa                                                                                                                           >  8:166479/1‑149 (MQ=255)                      ggcgccAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTCGTCGCCACGGACGATCACGGCACTCAGCTTGCCCTCTTCGGCACCGCGTACg                                                                                                                            >  8:274532/1‑148 (MQ=255)                         gacAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGAACCGCGTACGATCa                                                                                                                        <  7:274532/147‑1 (MQ=255)                           cAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAgg                                                                                                                      <  4:77301/149‑1 (MQ=255)                           cAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAgg                                                                                                                      >  1:187977/1‑149 (MQ=255)                              tGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTc                                                                                                                   >  5:250350/1‑149 (MQ=255)                                   tCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGAc                                                                                                              <  7:166479/149‑1 (MQ=255)                                     gcgcAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAAGCTGATTGCCctga                                                                                                                                           <  7:288062/118‑3 (MQ=255)                                     gcgcAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAAGCTGATTGCCctga                                                                                                                                           >  8:288062/1‑116 (MQ=255)                                       gcAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTc                                                                                                          <  3:218800/149‑1 (MQ=255)                                           ttCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCtttt                                                                                                      <  1:103852/149‑1 (MQ=255)                                           ttCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCtttt                                                                                                      >  2:255442/1‑149 (MQ=255)                                              ggCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGa                                                                                                   >  2:68530/1‑149 (MQ=255)                                                cTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATc                                                                                                 <  4:34938/149‑1 (MQ=255)                                                   cGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCgg                                                                                               >  7:30391/1‑148 (MQ=255)                                                     gTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAg                                                                                            >  6:77493/1‑149 (MQ=255)                                                           aTGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTg                                                                                      <  5:244737/149‑1 (MQ=255)                                                             gACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGt                                                                                    >  7:87469/1‑149 (MQ=255)                                                                  gCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTcc                                                                               <  5:77493/149‑1 (MQ=255)                                                                  gCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTcc                                                                               <  1:68530/149‑1 (MQ=255)                                                                  gCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTc                                                                                >  2:22150/1‑148 (MQ=255)                                                                      gTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTccacc                                                                            >  5:317595/1‑148 (MQ=255)                                                                          gCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTg                                                                       >  4:205906/1‑149 (MQ=255)                                                                            aaCCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGTACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTggg                                                                     >  6:95509/1‑149 (MQ=255)                                                                            aaCCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGc                                                                                              >  1:173500/1‑124 (MQ=255)                                                                            aaCCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGc                                                                                              <  2:173500/124‑1 (MQ=255)                                                                             aCCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTggg                                                                     >  4:96025/1‑148 (MQ=255)                                                                             aCCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTggg                                                                     >  2:53384/1‑148 (MQ=255)                                                                              ccTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTc                                                                                <  5:284250/136‑1 (MQ=255)                                                                              ccTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTc                                                                                >  6:284250/1‑136 (MQ=255)                                                                               cTGTTCCAGCTTGGTGGCCATGATTTCGTTCAGATCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGACTTTTCGATCGGCAGGCCGTGGTTTTCAACCAACAGGGCaa                                                                  >  2:41032/1‑149 (MQ=255)                                                                                  ttCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATg                                                                <  8:87469/148‑1 (MQ=255)                                                                                    ccAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTc                                                             >  1:32376/1‑149 (MQ=255)                                                                                       gCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTg                                                          <  1:255442/149‑1 (MQ=255)                                                                                             tggCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTc                                                    <  2:187977/149‑1 (MQ=255)                                                                                             tggCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCCTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTc                                                    >  4:175860/1‑149 (MQ=255)                                                                                               tgcTTGATTTGGTTCAGTTCGTGCTCGCGACGGACGATATTGGCAAGAAGGTTGCCCTCTTCGGCACCGCGTTCGATCAGGGTCTTGACGTTCTTTTGGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGTCATTCg                                                   <  8:125987/146‑1 (MQ=255)                                                                                                ccTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCggg                                                 <  6:317595/149‑1 (MQ=255)                                                                                                    gATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGgtgt                                             >  8:239235/1‑149 (MQ=255)                                                                                                     aTTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGgtgt                                             <  3:205906/148‑1 (MQ=255)                                                                                                     aTTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCCTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGgtgt                                             <  3:175860/148‑1 (MQ=255)                                                                                                          gTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATg                                                                >  7:98962/1‑124 (MQ=255)                                                                                                          gTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATg                                                                <  8:98962/124‑1 (MQ=255)                                                                                                           ttCAGTTCGTGGTCGCCACGGACGATCAGGCCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAg                                      >  1:26076/1‑149 (MQ=255)                                                                                                              aGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGgcg                                   <  3:96025/149‑1 (MQ=255)                                                                                                               gTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTc                                                                                <  8:252935/103‑1 (MQ=255)                                                                                                               gTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTc                                                                                >  7:252935/1‑103 (MQ=255)                                                                                                                ttCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGgcg                                   >  7:192889/1‑147 (MQ=255)                                                                                                                     ggTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGctcc                            >  7:11496/1‑149 (MQ=255)                                                                                                                       tCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCGGctcctc                          >  3:128147/1‑149 (MQ=255)                                                                                                                                aCGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGgtgt                                             <  8:172287/121‑1 (MQ=255)                                                                                                                                aCGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGgtgt                                             >  7:172287/1‑121 (MQ=255)                                                                                                                                  gaagaGGGCAAGCTGATTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAg               >  2:249476/5‑149 (MQ=255)                                                                                                                                   aagaGGGCAAGCTGATTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAgg              >  5:126831/4‑149 (MQ=255)                                                                                                                                   aagaGGGCAAGCTGATTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAgg              >  1:317859/4‑149 (MQ=255)                                                                                                                                        ggCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCa         >  3:309489/1‑149 (MQ=255)                                                                                                                                           aaTCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTg                                                          <  6:190436/97‑1 (MQ=255)                                                                                                                                           aaTCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTg                                                          >  5:190436/1‑97 (MQ=255)                                                                                                                                             tCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCACGGtt    <  1:22150/149‑1 (MQ=255)                                                                                                                                               aGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCACGGTTTc  <  8:11496/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                     CGGGCCGAGCGAGCCGGCGCCGGCGCCAATGGCGTCGCGCAGTTCGGCTTCGGTGGCCATGACCAGCGGGTCGGCAACCTGTTCCAGCTTGGTGGCCTTGATTTCGTTCAGTTCGTGGTCGCCACGGACGATCAGGGCAATCAGCTTGCCCTCTTCGGCACCGCGTACGATCAGGGTCTTGACGGTCTTTTCGATCGGCAGGCCGTGGTTTTCCACCAACTGGGCAATGGTCTTGGCATTCGGGGTGTCGACCAGGCGCAGCTCCTCGGTAGGGGCAGGGCGCACGGTTTC  >  NC_002947/1384017‑1384307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑