| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,400,876 | T→G | 8.3% | intergenic (+39/‑130) | ybgF → / → queE | TolQRA transport system periplasmic protein/7‑carboxy‑7‑deazaguanine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,400,876 | 0 | T | G | 8.3% | 238.8 / 11.9 | 84 | intergenic (+39/‑130) | ybgF/queE | TolQRA transport system periplasmic protein/7‑carboxy‑7‑deazaguanine synthase |
| Reads supporting (aligned to +/- strand): ref base T (34/43); new base G (3/4); total (37/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01 | |||||||||||
CGTATGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGACAC > NC_002947/1400730‑1401016 | cGTATGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTcg > 8:77436/1‑149 (MQ=255) gTATGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTcgc < 1:136269/149‑1 (MQ=255) gCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGttt > 5:76609/1‑149 (MQ=255) gCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGttt > 2:17492/1‑149 (MQ=255) gCCATACCAACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGttt > 6:125655/1‑149 (MQ=255) ccATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAA‑AAACCCGCGACTGGCGCGGGtttct < 5:227732/149‑3 (MQ=255) cATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAA‑AAACCCGCGACTGGCGCGGGtttc‑tt < 5:173158/149‑1 (MQ=255) tACCGACAAGGTAAAAGGCATCCTTCAGCAGGGCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTc < 7:126161/149‑1 (MQ=255) aCCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGTGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGCAACCCGCGCCAGTCGCGGGTTTT‑TTCg > 7:190770/1‑149 (MQ=255) cGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGtt < 2:62675/149‑1 (MQ=255) gACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGt > 3:246528/1‑147 (MQ=255) aCAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGt > 6:318337/1‑146 (MQ=255) aCAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGt > 2:27749/1‑146 (MQ=255) aCAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAg < 6:68916/149‑1 (MQ=255) gcctatAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTa < 2:292641/142‑1 (MQ=255) cAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGa < 5:318337/149‑1 (MQ=255) aaGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGaa < 4:218781/149‑1 (MQ=255) gTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCa < 1:17492/149‑1 (MQ=255) gTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCGCGAAAGAACCCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCa < 5:320080/149‑1 (MQ=255) aaaGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACt < 5:302476/148‑1 (MQ=255) aTCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCttt > 2:161811/1‑149 (MQ=255) tCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCtttt < 7:77436/149‑1 (MQ=255) cagcagGTCGTGACCCAGTACCGCGGCACCTCTTCCGCTCAACTGTCCCATCGTGACCTGCATAAGCTCTAGGCGTTGTCGCTGTACCTCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTc < 6:202197/147‑1 (MQ=255) cagcagGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTc < 4:33489/147‑1 (MQ=255) agcagGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTa < 2:272509/149‑1 (MQ=255) agcagGTCGTGACACAGTACCCCGGCACCTCTGAAGATCAACTGGCCCATCGTGACCTGCAGAAGCTCTAGTCGTTGTAGCTGTACATCTAGAAAGAAAACCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTTCCCTATTTTCGt > 6:328109/1‑148 (MQ=255) gcagGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTaa < 7:104316/149‑1 (MQ=255) gcagGGCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTaa < 6:198090/149‑1 (MQ=255) cagGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTaaa < 6:36279/149‑1 (MQ=255) tCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACg > 7:294389/1‑149 (MQ=255) tCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACg < 1:59671/149‑1 (MQ=255) gTGACCCAGTA‑CCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACgct > 8:111302/1‑148 (MQ=255) tGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACgctg < 3:86415/149‑1 (MQ=255) ccAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCtgctgc > 2:7926/1‑149 (MQ=255) ccAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCtgctgc > 2:261056/1‑149 (MQ=255) cccGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAAc > 6:81865/1‑149 (MQ=255) cccGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAAc > 6:77135/1‑149 (MQ=255) gCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTa < 7:170504/149‑1 (MQ=255) cACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTAt < 5:125655/149‑1 (MQ=255) ccGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGTCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGa < 8:294389/146‑1 (MQ=255) cGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTcc < 7:53170/149‑1 (MQ=255) aaCTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGa > 7:50331/1‑148 (MQ=255) aaCTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGa < 8:50331/148‑1 (MQ=255) aCTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGa > 5:79574/1‑147 (MQ=255) gCCCAGCGTGACCTGCAGAAGCTCTAGGAGTTGTCGCTTTACCGCTCGAAATAAACCCGCGTCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAAAACGCTGCTTCGTATAACG‑‑CTATGTCGAGTCCGCGACAGt < 5:328109/147‑1 (MQ=255) cccAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGcc > 1:216655/1‑149 (MQ=255) ccAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCt > 3:47126/1‑149 (MQ=255) tGACCTGCAGAAGCTCTAGGCGTAGTCGCTGTACCGCTCGAAAGAAACCCGCGCCTGTCGCGGTTTTT‑TTCGTTAGAATCACTGCACTTTTTTCGACCCCACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGa < 6:265806/149‑1 (MQ=255) aCCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAg < 7:111302/148‑1 (MQ=255) cAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGa > 7:112572/1‑148 (MQ=255) aaGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGc < 1:161811/149‑1 (MQ=255) aGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGc < 4:246528/148‑1 (MQ=255) aGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGc < 4:47126/148‑1 (MQ=255) gCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGct > 8:253448/1‑147 (MQ=255) gCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCt < 6:79574/149‑1 (MQ=255) ctAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGtt > 1:192037/1‑149 (MQ=255) ggCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACa > 6:45538/1‑119 (MQ=255) ggCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACa < 5:45538/119‑1 (MQ=255) gCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGc < 2:255030/149‑1 (MQ=255) gTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCa > 5:308842/1‑149 (MQ=255) tCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCNGACGGAGGCGGACAGCCTGTTTAGCTGTCa < 1:27749/148‑1 (MQ=255) gCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGAAAGTGCATGACGGAGGCGGACAGCCTGTTTAGCGGTCACGc > 6:316898/1‑149 (MQ=255) tACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGccc < 2:216655/147‑1 (MQ=255) ccGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTTATTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCGGGACGGAGGCGGACAGCGTGTTTAGCTGTCACGCCCggg > 1:49499/1‑147 (MQ=255) ccGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTg < 5:81865/148‑1 (MQ=255) ccGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTATCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTg < 5:77135/148‑1 (MQ=255) cGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACCGCCTGTTTAGCTGTCACGCCCGTGGc > 4:76942/1‑149 (MQ=255) cTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTTGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGc > 6:117270/1‑147 (MQ=255) gaaagaaaCCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACGCACTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGa < 1:261056/148‑1 (MQ=255) gaaagaaaCCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGc < 6:308842/148‑1 (MQ=255) gaaagaaaCCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCa > 8:220328/1‑149 (MQ=255) gaaagaaaCCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCa > 2:95411/1‑149 (MQ=255) gaaaaaaCCCGCGACTGGCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCAt > 3:39204/5‑149 (MQ=255) gaaaaaaCCCGCGACTGGCGCGGGTTTT‑TTCGCTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGa < 1:259503/141‑1 (MQ=255) gaaaaaaCCCGCGACTGGCGCGGGTTTT‑TTCGATAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGa > 2:259503/5‑145 (MQ=255) aaagaaaCCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCAt > 7:231183/1‑149 (MQ=255) aaagaaaCCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCAt > 7:92713/1‑149 (MQ=255) aaagaaaCCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCAt < 8:94634/149‑1 (MQ=255) aagaaaCCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATg < 6:76609/149‑1 (MQ=255) agaaaCCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGc < 2:192037/149‑1 (MQ=255) aaaaCCCGCGACTGGCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGt > 6:280864/2‑120 (MQ=255) aaaaCCCGCGACTGGCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGt < 5:280864/119‑1 (MQ=255) cgcgCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGacac < 8:112572/149‑1 (MQ=255) cgcgCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGaca > 7:173778/1‑148 (MQ=255) | CGTATGGGCCATACCGACAAGGTAAAAGGCATCCTTCAGCAGGTCGTGACCCAGTACCCCGGCACCTCTGCCGCTCAACTGGCCCAGCGTGACCTGCAGAAGCTCTAGGCGTTGTCGCTGTACCGCTCGAAAGAAACCCGCGCCAGTCGCGGGTTTT‑TTCGTTAGAATCACTGCCCTTTTTTCGTAAACACGCTGCTGCGGATAACG‑‑CTATGTCGAGTCCGCGACAGTGCCTGACGGAGGCGGACAGCCTGTTTAGCTGTCACGCCCGTGGCGAGCATGCAAGACAC > NC_002947/1400730‑1401016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |