Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,435,822	A→T	9.8%	C48S (TGC→AGC)	PP_1256 ←	alpha‑ketoglutarate semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,435,822	0	A	T	9.8%	158.1 / 8.0	61	C48S (TGC→AGC)	PP_1256	alpha‑ketoglutarate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (27/28);  new base T (3/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01

CTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCC  >  NC_002947/1435676‑1435969                                                                                                                                                    |                                                                                                                                                    cTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAgg                                                                                                                                                   >  6:208325/1‑149 (MQ=255)      gCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTgg                                                                                                                                              <  7:165604/149‑1 (MQ=255)      gCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTgg                                                                                                                                              <  6:259878/149‑1 (MQ=255)       cAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCTGCGCGCTGGCCTggg                                                                                                                                             <  1:27442/149‑1 (MQ=255)         ggCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCTGCGCGCTGGCCTGGGcg                                                                                                                                           <  4:283107/149‑2 (MQ=255)              gTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACgtg                                                                                                                                      >  7:194060/1‑149 (MQ=255)              gTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACgtg                                                                                                                                      >  5:126369/1‑149 (MQ=255)                ttCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGtt                                                                                                                                    >  1:22365/1‑149 (MQ=255)                     tCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCt                                                                                                                               >  2:148047/1‑149 (MQ=255)                                   aTCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCTGCGCGCTGGCCTGGGcg                                                                                                                                           >  3:311431/1‑122 (MQ=255)                                   aTCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCTGCGCGCTGGCCTGGGcg                                                                                                                                           <  4:311431/123‑2 (MQ=255)                                      aGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGc                                                                                                              <  2:22365/149‑1 (MQ=255)                                        ggCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCAc                                                                                                                                         >  3:201182/1‑120 (MQ=255)                                        ggCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCAc                                                                                                                                         <  4:201182/120‑1 (MQ=255)                                                  aGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGt                                                                                                  <  1:148047/149‑1 (MQ=255)                                                      ccTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAAc                                                                                                    >  3:74144/1‑143 (MQ=255)                                                      ccTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGACGCCGAGGTAGGCCGGCTCCAAc                                                                                                    <  4:74144/143‑1 (MQ=255)                                                       cTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGAc                                                                                             >  2:12380/1‑149 (MQ=255)                                                       cTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGAc                                                                                             >  2:12382/1‑149 (MQ=255)                                                               gtgtgGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGtt                                                                                     >  6:203430/1‑149 (MQ=255)                                                                tgtgGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCTCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGtt                                                                                     <  8:194060/148‑1 (MQ=255)                                                                tgtgGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGGGGTAGGCCGGCTCCAACGTCTGACCGGTGGtt                                                                                     <  5:208325/148‑1 (MQ=255)                                                                tgtgGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCgg                                                                                          <  6:266795/143‑1 (MQ=255)                                                                tgtgGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGtt                                                                                     <  1:122632/148‑1 (MQ=255)                                                                    gCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGt                                                                                 <  6:107877/148‑1 (MQ=255)                                                                    gCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGt                                                                                 <  5:203430/148‑1 (MQ=255)                                                                           cTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCggtg                                                                                        <  3:118256/134‑1 (MQ=255)                                                                           cTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCggtg                                                                                        >  4:118256/1‑134 (MQ=255)                                                                            ttCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCATGAGAATGCCGCACAGCCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCa                                                                         >  1:72343/1‑149 (MQ=255)                                                                               aaGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGa                                                                     >  5:84546/1‑149 (MQ=255)                                                                               aaGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGa                                                                     >  5:27183/1‑149 (MQ=255)                                                                                aGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGAt                                                                    <  3:200773/149‑1 (MQ=255)                                                                                 ggAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATg                                                                   <  7:51755/149‑1 (MQ=255)                                                                                 ggAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATg                                                                   <  7:56138/149‑1 (MQ=255)                                                                                          cTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCgg                                                          >  3:198070/1‑149 (MQ=255)                                                                                            cGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCt                                                        >  7:261350/1‑149 (MQ=255)                                                                                              ttgttCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGc                                                      <  1:12380/149‑1 (MQ=255)                                                                                              ttgttCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGc                                                      <  1:12382/149‑1 (MQ=255)                                                                                                gttCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCg                                                    >  1:193470/1‑149 (MQ=255)                                                                                                 ttCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCg                                                    <  6:27183/148‑1 (MQ=255)                                                                                                    gAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTa                                                >  6:144430/1‑149 (MQ=255)                                                                                                     aGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTAc                                               >  6:183084/1‑149 (MQ=255)                                                                                                        gATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGa                                                                                              <  5:75200/99‑1 (MQ=255)                                                                                                        gATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGa                                                                                              >  6:75200/1‑99 (MQ=255)                                                                                                        gATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCgg                                            >  3:181900/1‑149 (MQ=255)                                                                                                         aTGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTATGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGc                                           >  7:181736/1‑149 (MQ=255)                                                                                                                gcgGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTgg                                     <  6:126369/148‑1 (MQ=255)                                                                                                                gcgGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGc                                    <  5:144430/149‑1 (MQ=255)                                                                                                                gcgGGAGGCAT‑CGAATGCCGCCCCGGCCAGCTCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACTGCTGACCGGTGGTTGGGTAGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCctcttg                                     <  6:271638/148‑3 (MQ=255)                                                                                                                          t‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagcag                          <  8:165974/149‑1 (MQ=255)                                                                                                                          t‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATcagcag                          >  1:32597/1‑149 (MQ=255)                                                                                                                              aaTGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGtt                       <  4:198070/149‑1 (MQ=255)                                                                                                                               aTGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTg                      >  1:246893/1‑149 (MQ=255)                                                                                                                                tGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGc                     <  5:183084/149‑1 (MQ=255)                                                                                                                                   cgccCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCtg                  >  8:61395/1‑149 (MQ=255)                                                                                                                                       cAGGCCTGCGCGCTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAg              >  7:47872/1‑149 (MQ=255)                                                                                                                                            cAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCa         <  2:209373/149‑1 (MQ=255)                                                                                                                                            cAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTCCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCa         >  1:209373/1‑149 (MQ=255)                                                                                                                                             tgcgcgcTGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCAt        >  1:237717/2‑149 (MQ=255)                                                                                                                                                  gcAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGc   >  8:136353/1‑149 (MQ=255)                                                                                                                                                   cAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGcc  <  2:72343/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CTTGGGCAGGCCGGTTTCGATCACGGCGCGGTCGATCAGGGCATCGCCGAGGGCCTCGATCTGTGTGGCGATGGCTTCAAGGAACTCTGCTCGTTGTTCGAGCGATGTTTCGCGGTAGGCAT‑CGAATGCCGCCCAGGCCAGCGCGCAGGCCTGGGCCACGTGTTCGCCTGTGCCGCCGAGGTAGGCCGGCTCCAACGTCTGACCGGTGGTTGGGTCGATGGCACGGATGGCGTCGCGGCTGCCGGTTACCGGCCTCTGGCCGATCAGCAGGTTGCCTGTGAGGGGCATGTAGCC  >  NC_002947/1435676‑1435969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑