Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,446,879	C→T	6.1%	A326V (GCC→GTC)	PP_1264 →	fusaric acid resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,446,879	0	C	T	6.1%	193.6 / 2.1	65	A326V (GCC→GTC)	PP_1264	fusaric acid resistance protein
Reads supporting (aligned to +/- strand):  ref base C (30/31);  new base T (2/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGAGCTACGCCGAAACCCATGCCTCGCTGGCCGCGCA  >  NC_002947/1446739‑1447026                                                                                                                                                |                                                                                                                                                    gCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGc                                                                                                                                             <  7:250824/149‑1 (MQ=255)      aaaTTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCggggg                                                                                                                                        >  7:9619/1‑149 (MQ=255)              aGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGTCCAGGTGCGGGGGCTgcgggc                                                                                                                                >  6:38140/1‑149 (MQ=255)              aGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGTCCAGGTGCGGGGGCTgcgggc                                                                                                                                >  4:173468/1‑149 (MQ=255)                   tATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTACTGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTGCAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAg                                                                                                                            >  1:231274/1‑149 (MQ=255)                   tATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGCC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAg                                                                                                                            >  7:276426/1‑148 (MQ=255)                      cGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTAcc                                                                                                                        >  5:324048/1‑149 (MQ=255)                            gggCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCaa                                                                                                                  <  6:48549/149‑1 (MQ=255)                              gCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACg                                                                                                                <  8:123048/149‑1 (MQ=255)                                   aaTACCCTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTGCCAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGa                                                                                                              <  1:67469/147‑1 (MQ=255)                                   aaTACCCTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGAccc                                                                                                           <  1:132328/149‑1 (MQ=255)                                       cccTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGc                                                                                                       <  7:21602/149‑1 (MQ=255)                                        ccTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGc                                                                                                       >  3:43769/1‑148 (MQ=255)                                        ccTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGc                                                                                                       >  7:182644/1‑148 (MQ=255)                                          tggtggAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTgcgg                                                                                                                                  <  6:37277/119‑1 (MQ=255)                                          tggtggAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTgcgg                                                                                                                                  >  5:37277/1‑119 (MQ=255)                                            gtggAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCT‑GAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATcc                                                                                                 >  2:213741/1‑149 (MQ=255)                                              ggAGTTGCTGCAGCCCTA‑TGTGGGCCCGGCGATGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGCCCCAGGTGCGGGGGCTGCGGGCGGACTTCCTGGCAACGGACCCCAGCGAATACg                                                                                                >  7:227397/1‑149 (MQ=255)                                                aGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGAc                                                                                              <  8:182644/149‑1 (MQ=255)                                                    gctgcAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTAc                                                                                                                         >  8:181114/1‑118 (MQ=255)                                                    gctgcAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTAc                                                                                                                         <  7:181114/118‑1 (MQ=255)                                                     ctgcAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCt                                                                                         <  6:324048/149‑1 (MQ=255)                                                             cTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTcc                                                                                 <  4:140336/149‑1 (MQ=255)                                                             cTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTc                                                                                  >  3:140336/1‑148 (MQ=255)                                                             cTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTc                                                                                  >  3:185303/1‑148 (MQ=255)                                                                 tgtgGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATa                                                                              <  3:307861/149‑1 (MQ=255)                                                                   tgGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCT‑GAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATAcc                                                                            <  1:213741/148‑1 (MQ=255)                                                                    gggCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACcg                                                                           >  3:29717/1‑149 (MQ=255)                                                                     ggCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCggg                                                                                                                                          <  7:281435/85‑1 (MQ=255)                                                                     ggCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCggg                                                                                                                                          >  8:281435/1‑85 (MQ=255)                                                                      gCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACcgcg                                                                         >  7:50687/1‑149 (MQ=255)                                                                             gTTGACTGACGCCGATGCGCTGCGCCTGACGCCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAg                                                                   >  1:29681/1‑149 (MQ=255)                                                                              ttGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAgct                                                                 >  4:256494/1‑149 (MQ=255)                                                                                gactgacGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAgctgc                                                               >  7:195372/1‑149 (MQ=255)                                                                                   tgacGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGc                                                                                                                                       >  8:176115/1‑74 (MQ=255)                                                                                   tgacGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGc                                                                                                                                       <  7:176115/74‑1 (MQ=255)                                                                                    gacGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTa                                                           >  5:173558/1‑149 (MQ=255)                                                                                     acGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTGCAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGt                                                            <  2:231274/147‑1 (MQ=255)                                                                                        ccGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCGGCCTAGAAGGACGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCTATACCGCGTTCGAGCTGCTGTACCGc                                                       <  6:164293/149‑1 (MQ=255)                                                                                         cGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATcc                                                                                                 >  1:173647/1‑106 (MQ=255)                                                                                         cGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATcc                                                                                                 <  2:173647/106‑1 (MQ=255)                                                                                         cGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCt                                                      <  3:256494/149‑1 (MQ=255)                                                                                           aTGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGTCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTtc                                                    <  3:280977/149‑1 (MQ=255)                                                                                              cgctgcgcCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTtcgtc                                                 <  6:173558/149‑1 (MQ=255)                                                                                                ctgcgcCTGAC‑CCGGGAGCTGGCCGCCTTCACGGAAGGCCTG‑CTGGCCCAGGTGCGGGGGCTGCGGTGGGAGTAGCTGGCTACGGACGCGAGCTTATCCGACCTGCTCGTTTTCGATAGCGCGTTCGAGCTGCTGTACGGGTtcgtcgt                                               <  8:227397/149‑2 (MQ=255)                                                                                                 tgcgcCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTcgac                                              <  4:185303/149‑1 (MQ=255)                                                                                                 tgcgcCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTcgac                                              >  3:105309/1‑149 (MQ=255)                                                                                                  gcgcCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTcgac                                              >  2:12715/1‑148 (MQ=255)                                                                                                   cgcCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTcgacg                                             <  1:12715/148‑1 (MQ=255)                                                                                                   cgcCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTcgac                                              <  4:105309/147‑1 (MQ=255)                                                                                                    gcCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACgag                                           <  8:9619/149‑1 (MQ=255)                                                                                                            ccTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGa                                    <  8:50687/149‑1 (MQ=255)                                                                                                             cTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGAg                                   <  4:43769/149‑1 (MQ=255)                                                                                                               ggAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGAGc                                  >  8:110208/1‑148 (MQ=255)                                                                                                                gAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGTCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGAGCt                                 <  3:173468/148‑1 (MQ=255)                                                                                                                gAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGTCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGAGCt                                 <  5:38140/148‑1 (MQ=255)                                                                                                                           cTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACAGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGATCTACGCCGAAAcc                      >  3:34438/1‑148 (MQ=255)                                                                                                                              cAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGAGCTACGCCGAAACCCAt                   <  8:276426/148‑1 (MQ=255)                                                                                                                               aaggaaggCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGAGCTACGCCGAAACCCATGc                 >  5:85251/1‑149 (MQ=255)                                                                                                                                 ggaaggCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGa                                                                    >  2:207213/1‑96 (MQ=255)                                                                                                                                 ggaaggCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGAGCTACGCCGAAACCCATGCCt               >  2:17437/1‑149 (MQ=255)                                                                                                                                     ggCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGTTGTTGAGCTACGCCGAAACCCATGCCTCGCt           <  1:35386/149‑1 (MQ=255)                                                                                                                                       ccTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGAGCTACGCCGAAACCCATGCCTCGCTgg         <  2:29681/149‑1 (MQ=255)                                                                                                                                          g‑CAGGCCCAGGTGCGGGGGCTGAGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGAGCTACGCCGAAACCCATGCCTCTCTCGCcg      >  5:295546/1‑149 (MQ=255)                                                                                                                                               gCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGAGCTACGCCGAAACCCATGCCTCGCTGGCCGCGca  >  2:56658/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                    GCTGCAAATTGTCAGCGCTATCGAACCGGGCCTGAATACCCTGGTGGAGTTGCTGCAGCCCTA‑TGTGGGCCGGGCGTTGACTGACGCCGATGCGCTGCGCCTGAC‑CCTGGAGCTGGCCGCCTACAAGGAAGGCCTG‑CAGGCCCAGGTGCGGGGGCTGCGGGCGGAGTACCTGGCAACGGACCCCAGCGAATCCGACCTGCTCGATTTCCATACCGCGTTCGAGCTGCTGTACCGCTTCGTCGACGAGATGTTGAGCTACGCCGAAACCCATGCCTCGCTGGCCGCGCA  >  NC_002947/1446739‑1447026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑