Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 149,904 T→G 6.4% H349P (CAC→CCC)  PP_0142 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947149,9040TG 6.4% 191.8 / 3.5 62H349P (CAC→CCC) PP_0142ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (28/30);  new base G (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGA  >  NC_002947/149756‑150043
                                                                                                                                                    |                                                                                                                                           
ccaccTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGgtgt                                                                                                                                             <  3:245551/149‑1 (MQ=255)
    cTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGc                                                                                                                                         >  8:263031/1‑149 (MQ=255)
         ggcCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCa                                                                                                                                    >  1:259602/1‑149 (MQ=255)
           ccGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCAcc                                                                                                                                  <  1:310104/149‑1 (MQ=255)
               aGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACt                                                                                                                              <  2:259602/149‑1 (MQ=255)
                  ggTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCgg                                                                                                                           <  1:76764/149‑1 (MQ=255)
                   gTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGTCGGTGGGGGCACACACCACCTCGgc                                                                                                                          <  7:125171/149‑1 (MQ=255)
                       cAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTg                                                                                                                      >  7:199144/1‑149 (MQ=255)
                        aGATGCCGCGTTCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCTCATCCAGCAAGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGttgtgt                                                                                                                                           <  5:73913/127‑1 (MQ=255)
                        aGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGgtgtgt                                                                                                                                           >  6:78591/1‑127 (MQ=255)
                        aGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGGGgtgtgt                                                                                                                                           <  5:78591/127‑1 (MQ=255)
                           tGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGc                                                                                                                  <  3:76959/149‑1 (MQ=255)
                              cgctGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCAGGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGa                                                                                                               <  8:2833/149‑1 (MQ=255)
                               gcgGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCACCACCGACTCGGCGCTGCCGCTGAc                                                                                                              >  1:164621/1‑149 (MQ=255)
                                       cGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGaa                                                                                                        <  7:276986/147‑1 (MQ=255)
                                        gATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGcc                                                                                                     >  5:299413/1‑149 (MQ=255)
                                        gATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGcc                                                                                                     >  4:63974/1‑149 (MQ=255)
                                             cGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGaa                                                                                                        >  3:186214/1‑141 (MQ=255)
                                             cGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGaa                                                                                                        <  4:186214/141‑1 (MQ=255)
                                             cGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCa                                                                                                <  6:299413/149‑1 (MQ=255)
                                             cGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCa                                                                                                <  5:729/149‑1 (MQ=255)
                                                aaTTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCa                                                                                                >  5:250651/1‑146 (MQ=255)
                                                     tGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGcc                                                                                         >  8:41736/1‑148 (MQ=255)
                                                       gCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGAt                                                                                      <  7:263031/149‑1 (MQ=255)
                                                            tCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATggcgg                                                                                 >  2:57601/1‑149 (MQ=255)
                                                                 aGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATc                                                                            <  7:41736/149‑1 (MQ=255)
                                                                 aGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATc                                                                            <  1:57601/149‑1 (MQ=255)
                                                                   cccATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAg                                                                          <  7:176126/149‑1 (MQ=255)
                                                                     cATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAgg                                                                         >  3:259855/1‑148 (MQ=255)
                                                                     cATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGa                                                                        <  5:160683/149‑1 (MQ=255)
                                                                      aTCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAgg                                                                         >  4:192262/1‑147 (MQ=255)
                                                                          ccATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGAcc                                                                                                             <  5:134445/107‑1 (MQ=255)
                                                                          ccATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGAcc                                                                                                             >  6:134445/1‑107 (MQ=255)
                                                                           cATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCtt                                                                                                   <  6:55949/116‑1 (MQ=255)
                                                                           cATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCtt                                                                                                   >  5:55949/1‑116 (MQ=255)
                                                                            aTGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGCCTCGCCGCTGCCGGTGCCATTCAAGCCTTCCAGCCAGCCGATGGCGGCGAGCAGGAAGGCaa                                                                  >  7:188138/1‑148 (MQ=255)
                                                                               aagaaCAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGaa                                                              >  5:79852/1‑149 (MQ=255)
                                                                                agaaCAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAAc                                                             <  3:192262/149‑1 (MQ=255)
                                                                                 gaaCAGCGCTGCCACCGCATCCAGCACGAGTACCACAAATATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACg                                                            <  2:164621/149‑1 (MQ=255)
                                                                                       cgcTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCt                                                      >  3:265272/1‑149 (MQ=255)
                                                                                       cgcTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCt                                                      <  4:259855/149‑1 (MQ=255)
                                                                                            ccACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGc                                                  >  6:12269/1‑148 (MQ=255)
                                                                                                cGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGc                                             <  4:243430/149‑1 (MQ=255)
                                                                                                    tCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGt                                                          <  5:96085/132‑1 (MQ=255)
                                                                                                    tCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGt                                                          >  6:96085/1‑132 (MQ=255)
                                                                                                       aGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAg                                      <  8:199144/149‑1 (MQ=255)
                                                                                                            gATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTg                                                    <  1:133257/130‑1 (MQ=255)
                                                                                                            gATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTg                                                    >  2:133257/1‑130 (MQ=255)
                                                                                                             aTGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGtg                                <  6:79852/149‑1 (MQ=255)
                                                                                                              tGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGtgc                               <  5:12269/149‑1 (MQ=255)
                                                                                                                 ccgcaAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAg                                                                      <  2:290095/104‑1 (MQ=255)
                                                                                                                 ccgcaAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAg                                                                      >  1:290095/4‑107 (MQ=255)
                                                                                                                 ccACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGtgctgc                            <  4:265272/149‑1 (MQ=255)
                                                                                                                 ccACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGtgctg                             >  2:229205/1‑148 (MQ=255)
                                                                                                                   acaAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCAc                          >  1:78959/1‑149 (MQ=255)
                                                                                                                   acaAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCAc                          >  6:240024/1‑149 (MQ=255)
                                                                                                                         aTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAAt                    <  7:220520/149‑1 (MQ=255)
                                                                                                                               tGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCt              >  7:243189/1‑149 (MQ=255)
                                                                                                                                    cagcgCCGAGTCGGTGGGGGCACACACCACCTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTg           >  6:299810/5‑147 (MQ=38)
                                                                                                                                      gcgCCGAGTCGGTGGGGGCACACACCACCTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTgcagc       <  5:299810/147‑1 (MQ=255)
                                                                                                                                         ccGAGTCGGTGGGGGCACACACCACCTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCagcagc    >  8:223707/1‑149 (MQ=255)
                                                                                                                                         ccGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTgcagca      >  2:208127/1‑147 (MQ=255)
                                                                                                                                         ccGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTgcagca      <  1:208127/147‑1 (MQ=255)
                                                                                                                                           gAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCtt             <  7:240313/138‑1 (MQ=255)
                                                                                                                                           gAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCtt             >  8:240313/1‑138 (MQ=255)
                                                                                                                                            aGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGa  >  4:405/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                           
CCACCTGGCGGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGA  >  NC_002947/149756‑150043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑