Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,524,047 G→C 6.5% A251P (GCT→CCT) ‡ murG → UDP‑N‑acetylglucosamine‑‑N‑acetylmuramyl‑ (pentapeptide) pyrophosphoryl‑undecaprenol N‑acetylglucosamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,524,0470GC 6.5% 240.5 / 6.8 77A251P (GCT→CCT) ‡murGUDP‑N‑acetylglucosamine‑‑N‑acetylmuramyl‑ (pentapeptide) pyrophosphoryl‑undecaprenol N‑acetylglucosamine transferase
Reads supporting (aligned to +/- strand):  ref base G (34/38);  new base C (2/3);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-01

CCTAAGGCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGCCC  >  NC_002947/1523900‑1524191
                                                                                                                                                   |                                                                                                                                                 
ccTAAGGCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGc                                                                                                                                                  >  1:218341/1‑149 (MQ=255)
 cTAAGGCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCt                                                                                                                                                 >  7:116298/1‑149 (MQ=255)
    aGGCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGTTCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCtt                                                                                                                                                <  4:164548/147‑1 (MQ=255)
      gCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATgg                                                                                                                                            >  5:155340/1‑149 (MQ=255)
        ccTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCt                                                                                                                                          >  8:42986/1‑149 (MQ=255)
           gTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGTCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTAGCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAATGAGATGGCCGAGGCTTATGGCTgg                                                                                                                                        >  7:108411/1‑148 (MQ=255)
           gTCCGAGGTGACCGCGGCGCTGCTTGCAGAGGGTTTCCACGAGGCGGGCAAGGAGCACGCACCGATCACCGCCGATCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTgg                                                                                                                                        <  8:108411/148‑1 (MQ=255)
            tCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTggg                                                                                                                                       <  7:22742/148‑1 (MQ=255)
            tCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑c                                                                                                                                     >  4:124775/1‑149 (MQ=255)
              cGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCGCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGCCATAAGCCTGGG‑cc                                                                                                                                    <  8:285489/148‑1 (MQ=255)
                aGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGAc                                                                                                                                 <  3:291003/149‑1 (MQ=255)
                 ggTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGCCTGGG‑CCGAc                                                                                                                                 >  8:106755/1‑148 (MQ=255)
                  gTGCCCGCTGCGCTGCGGCCAGAGGTGTTCCACCAGTCCGGCAAGCATCACGCACCGATCACCGCCGAGCTTTATCACGAGGCGGGCGTCGCGGCTCATGTAGAGCCTTTCATCAAATACATGGCCCAGCCATAAGCCTGGG‑ccatgtc                                                                                                                               <  4:218165/149‑6 (MQ=255)
                     cccGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCtggt                                                                                                                            <  7:42986/149‑1 (MQ=255)
                     cccGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCtgg                                                                                                                             <  3:124775/148‑1 (MQ=255)
                      ccGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCtggtg                                                                                                                           <  1:137367/149‑1 (MQ=255)
                        gcggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTggtggt                                                                                                                         >  1:38383/1‑149 (MQ=255)
                         cggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGgtggtg                                                                                                                        >  7:26920/1‑149 (MQ=255)
                          ggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGgtgt                                                                                                                       <  6:310723/149‑1 (MQ=255)
                          ggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGgtgt                                                                                                                       <  2:218341/149‑1 (MQ=255)
                          ggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGgtgt                                                                                                                       <  7:216305/149‑1 (MQ=255)
                               tGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTgccgg                                                                                                                  >  3:106159/1‑149 (MQ=255)
                                   gCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACACGGCCCAGCCATAAGCCTGGG‑ccat                                                                                                                                  >  3:156618/1‑127 (MQ=255)
                                   gCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACACGGCCCAGCCATAAGCCTGGG‑ccat                                                                                                                                  <  4:156618/129‑3 (MQ=255)
                                    ccAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTgccgggccgg                                                                                                             >  7:187697/1‑149 (MQ=255)
                                      agagGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTg                                                                                                           >  4:211475/1‑149 (MQ=255)
                                        agGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGcc                                                                                                         >  2:248581/1‑149 (MQ=255)
                                         ggTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCt                                                                                                        <  2:28888/149‑1 (MQ=255)
                                             ttCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTTGAGCCTTTCATCAATGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGAc                                                                                                    <  7:106755/149‑1 (MQ=255)
                                             ttCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGa                                                                                                     <  4:106159/148‑1 (MQ=255)
                                               ccaccaGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTg                                                                                                  >  7:301628/1‑149 (MQ=255)
                                               ccaccaGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTg                                                                                                  >  3:146110/1‑149 (MQ=255)
                                                  ccaGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTct                                                                                               >  1:293890/1‑148 (MQ=255)
                                                      gCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCAGGGCCGGTGCCTTGACTGTCAGCg                                                                                            >  2:17536/1‑148 (MQ=255)
                                                           cAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTgg                                                                                      <  4:146110/149‑1 (MQ=255)
                                                             agcagcACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGgca                                                                                    <  8:26920/149‑1 (MQ=255)
                                                                  cacgcacCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGgcagcag                                                                                <  1:248581/149‑1 (MQ=255)
                                                                      cacCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGgc                                                                           <  5:328/149‑1 (MQ=255)
                                                                      cacCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGgc                                                                           <  2:38383/149‑1 (MQ=255)
                                                                        ccGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCTGGTGCCTTGACTGTCAGCTAACTGGCAGCAGCAGgcct                                                                         <  3:229890/149‑1 (MQ=255)
                                                                         agATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGgcctg                                                                        >  2:27042/2‑149 (MQ=255)
                                                                              aCCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCtt                                                                    <  8:301628/148‑1 (MQ=255)
                                                                              aCCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTc                                                                   <  6:155340/149‑1 (MQ=255)
                                                                              aCCGCCGAGCGTTATCACGAGGCGGGCGTCGCGCCTCAGCTAGAGCCATTCATCAAAGACATGGCACAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCACCAGCAGGCCTGCCTTc                                                                   >  1:44550/1‑149 (MQ=255)
                                                                                cgccgAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGa                                                                 <  5:263556/149‑1 (MQ=255)
                                                                                cgccgAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGa                                                                 >  4:286245/1‑149 (MQ=255)
                                                                                  ccgAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATg                                                               >  1:123001/1‑149 (MQ=255)
                                                                                       cGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGt                                                          >  3:176056/1‑149 (MQ=255)
                                                                                                gAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGcc                                                 <  2:229708/149‑1 (MQ=255)
                                                                                                  ggcgggcgTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGccc                                                >  1:189066/1‑148 (MQ=255)
                                                                                                     gggcgTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCAcg                                            >  1:269807/1‑149 (MQ=255)
                                                                                                          tCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATc                                       <  2:189066/149‑1 (MQ=255)
                                                                                                          tCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATc                                       <  2:123001/149‑1 (MQ=255)
                                                                                                             cgGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCCGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATcgac                                    <  5:305757/149‑1 (MQ=255)
                                                                                                             cgGCTCAGCTAGATCCTTTCATCAAAGACATGGCCCCGGCTTATGGCTTGG‑CCGACCTGGGGTGGGGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATcgac                                    <  8:230624/149‑1 (MQ=255)
                                                                                                                cTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGGCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGAt                                 <  3:275376/149‑1 (MQ=255)
                                                                                                                 tCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGAt                                 >  3:203699/1‑148 (MQ=255)
                                                                                                                 tCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGAt                                 >  7:216146/1‑148 (MQ=255)
                                                                                                                   aGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATc                                <  6:9515/147‑1 (MQ=255)
                                                                                                                    ggTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGNTGCCTTTGCCCCACGCGATCGACGATCAcc                             <  1:27042/149‑1 (MQ=255)
                                                                                                                           ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGa                                         <  1:145072/130‑1 (MQ=255)
                                                                                                                           ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGa                                         >  2:145072/1‑130 (MQ=255)
                                                                                                                           ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATcga                                     >  2:169035/1‑134 (MQ=255)
                                                                                                                           ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATcga                                     <  1:169035/134‑1 (MQ=255)
                                                                                                                           ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTca                      <  8:216146/149‑1 (MQ=255)
                                                                                                                             tttCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTcac                     >  6:246274/1‑148 (MQ=255)
                                                                                                                                 atcaAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTAGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGc                <  6:240208/149‑1 (MQ=255)
                                                                                                                                    aaaGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGccc              >  6:58808/1‑148 (MQ=255)
                                                                                                                                       gACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGACCGGTGCCTTGACTGTCAGCGAACTGGCAGCGGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAata          >  4:78760/1‑149 (MQ=255)
                                                                                                                                         gtcggCCCAGCCATAAGCCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCATGCGATCGACGATCACCAGACTCACAACGCCCAATATc        >  8:194893/4‑149 (MQ=255)
                                                                                                                                          aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTTCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCt       <  8:116298/149‑1 (MQ=255)
                                                                                                                                          aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAatat         >  4:225271/1‑147 (MQ=255)
                                                                                                                                          aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCt       <  8:187697/149‑1 (MQ=255)
                                                                                                                                          aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCt       >  5:250077/1‑149 (MQ=255)
                                                                                                                                          aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGACCGGTGCCTTGACTGTCAGCGAACTGGCAGCGGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCt       <  3:78760/149‑1 (MQ=255)
                                                                                                                                             gCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGc    <  3:211475/149‑1 (MQ=255)
                                                                                                                                               ccAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGccc  >  1:291840/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CCTAAGGCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGCCC  >  NC_002947/1523900‑1524191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑