Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,524,049 T→A 6.7% A251A (GCT→GCA) ‡ murG → UDP‑N‑acetylglucosamine‑‑N‑acetylmuramyl‑ (pentapeptide) pyrophosphoryl‑undecaprenol N‑acetylglucosamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,524,0490TA 6.7% 217.1 / 5.0 75A251A (GCT→GCA) ‡murGUDP‑N‑acetylglucosamine‑‑N‑acetylmuramyl‑ (pentapeptide) pyrophosphoryl‑undecaprenol N‑acetylglucosamine transferase
Reads supporting (aligned to +/- strand):  ref base T (32/38);  new base A (2/3);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-01

CTAAGGCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGCCCGCGAA  >  NC_002947/1523901‑1524196
                                                                                                                                                    |                                                                                                                                                    
cTAAGGCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCt                                                                                                                                                      >  7:116298/1‑149 (MQ=255)
   aGGCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGTTCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCtt                                                                                                                                                     <  4:164548/147‑1 (MQ=255)
     gCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATgg                                                                                                                                                 >  5:155340/1‑149 (MQ=255)
       ccTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCt                                                                                                                                               >  8:42986/1‑149 (MQ=255)
          gTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGTCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTAGCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAATGAGATGGCCGAGGCTTATGGCTgg                                                                                                                                             >  7:108411/1‑148 (MQ=255)
          gTCCGAGGTGACCGCGGCGCTGCTTGCAGAGGGTTTCCACGAGGCGGGCAAGGAGCACGCACCGATCACCGCCGATCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTgg                                                                                                                                             <  8:108411/148‑1 (MQ=255)
           tCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTggg                                                                                                                                            <  7:22742/148‑1 (MQ=255)
           tCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑c                                                                                                                                          >  4:124775/1‑149 (MQ=255)
             cGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCGCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGCCATAAGCCTGGG‑cc                                                                                                                                         <  8:285489/148‑1 (MQ=255)
               aGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGAc                                                                                                                                      <  3:291003/149‑1 (MQ=255)
                ggTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGCCTGGG‑CCGAc                                                                                                                                      >  8:106755/1‑148 (MQ=255)
                 gTGCCCGCTGCGCTGCGGCCAGAGGTGTTCCACCAGTCCGGCAAGCATCACGCACCGATCACCGCCGAGCTTTATCACGAGGCGGGCGTCGCGGCTCATGTAGAGCCTTTCATCAAATACATGGCCCAGCCATAAGCCTGGG‑ccatgtc                                                                                                                                    <  4:218165/149‑6 (MQ=255)
                    cccGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCtggt                                                                                                                                 <  7:42986/149‑1 (MQ=255)
                    cccGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCtgg                                                                                                                                  <  3:124775/148‑1 (MQ=255)
                     ccGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCtggtg                                                                                                                                <  1:137367/149‑1 (MQ=255)
                       gcggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTggtggt                                                                                                                              >  1:38383/1‑149 (MQ=255)
                        cggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGgtggtg                                                                                                                             >  7:26920/1‑149 (MQ=255)
                         ggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGgtgt                                                                                                                            <  6:310723/149‑1 (MQ=255)
                         ggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGgtgt                                                                                                                            <  7:216305/149‑1 (MQ=255)
                         ggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGgtgt                                                                                                                            <  2:218341/149‑1 (MQ=255)
                              tGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTgccgg                                                                                                                       >  3:106159/1‑149 (MQ=255)
                                  gCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACACGGCCCAGCCATAAGCCTGGG‑ccat                                                                                                                                       >  3:156618/1‑127 (MQ=255)
                                  gCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACACGGCCCAGCCATAAGCCTGGG‑ccat                                                                                                                                       <  4:156618/129‑3 (MQ=255)
                                   ccAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTgccgggccgg                                                                                                                  >  7:187697/1‑149 (MQ=255)
                                     agagGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTg                                                                                                                >  4:211475/1‑149 (MQ=255)
                                       agGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGcc                                                                                                              >  2:248581/1‑149 (MQ=255)
                                        ggTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCt                                                                                                             <  2:28888/149‑1 (MQ=255)
                                            ttCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTTGAGCCTTTCATCAATGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGAc                                                                                                         <  7:106755/149‑1 (MQ=255)
                                            ttCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGa                                                                                                          <  4:106159/148‑1 (MQ=255)
                                              ccaccaGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTg                                                                                                       >  3:146110/1‑149 (MQ=255)
                                              ccaccaGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTg                                                                                                       >  7:301628/1‑149 (MQ=255)
                                                 ccaGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTct                                                                                                    >  1:293890/1‑148 (MQ=255)
                                                     gCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCAGGGCCGGTGCCTTGACTGTCAGCg                                                                                                 >  2:17536/1‑148 (MQ=255)
                                                          cAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTgg                                                                                           <  4:146110/149‑1 (MQ=255)
                                                            agcagcACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGgca                                                                                         <  8:26920/149‑1 (MQ=255)
                                                                 cacgcacCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGgcagcag                                                                                     <  1:248581/149‑1 (MQ=255)
                                                                     cacCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGgc                                                                                <  5:328/149‑1 (MQ=255)
                                                                     cacCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGgc                                                                                <  2:38383/149‑1 (MQ=255)
                                                                       ccGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCTGGTGCCTTGACTGTCAGCTAACTGGCAGCAGCAGgcct                                                                              <  3:229890/149‑1 (MQ=255)
                                                                        agATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGgcctg                                                                             >  2:27042/2‑149 (MQ=255)
                                                                             aCCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCtt                                                                         <  8:301628/148‑1 (MQ=255)
                                                                             aCCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTc                                                                        <  6:155340/149‑1 (MQ=255)
                                                                             aCCGCCGAGCGTTATCACGAGGCGGGCGTCGCGCCTCAGCTAGAGCCATTCATCAAAGACATGGCACAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCACCAGCAGGCCTGCCTTc                                                                        >  1:44550/1‑149 (MQ=255)
                                                                               cgccgAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGa                                                                      >  4:286245/1‑149 (MQ=255)
                                                                               cgccgAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGa                                                                      <  5:263556/149‑1 (MQ=255)
                                                                                 ccgAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATg                                                                    >  1:123001/1‑149 (MQ=255)
                                                                                      cGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGt                                                               >  3:176056/1‑149 (MQ=255)
                                                                                               gAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGcc                                                      <  2:229708/149‑1 (MQ=255)
                                                                                                 ggcgggcgTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGccc                                                     >  1:189066/1‑148 (MQ=255)
                                                                                                    gggcgTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCAcg                                                 >  1:269807/1‑149 (MQ=255)
                                                                                                         tCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATc                                            <  2:189066/149‑1 (MQ=255)
                                                                                                         tCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATc                                            <  2:123001/149‑1 (MQ=255)
                                                                                                            cgGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCCGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATcgac                                         <  5:305757/149‑1 (MQ=255)
                                                                                                            cgGCTCAGCTAGATCCTTTCATCAAAGACATGGCCCCGGCTTATGGCTTGG‑CCGACCTGGGGTGGGGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATcgac                                         <  8:230624/149‑1 (MQ=255)
                                                                                                               cTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGGCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGAt                                      <  3:275376/149‑1 (MQ=255)
                                                                                                                tCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGAt                                      >  3:203699/1‑148 (MQ=255)
                                                                                                                tCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGAt                                      >  7:216146/1‑148 (MQ=255)
                                                                                                                  aGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATc                                     <  6:9515/147‑1 (MQ=255)
                                                                                                                   ggTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGNTGCCTTTGCCCCACGCGATCGACGATCAcc                                  <  1:27042/149‑1 (MQ=255)
                                                                                                                          ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGa                                              <  1:145072/130‑1 (MQ=255)
                                                                                                                          ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGa                                              >  2:145072/1‑130 (MQ=255)
                                                                                                                          ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATcga                                          >  2:169035/1‑134 (MQ=255)
                                                                                                                          ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATcga                                          <  1:169035/134‑1 (MQ=255)
                                                                                                                          ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTca                           <  8:216146/149‑1 (MQ=255)
                                                                                                                            tttCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTcac                          >  6:246274/1‑148 (MQ=255)
                                                                                                                                atcaAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTAGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGc                     <  6:240208/149‑1 (MQ=255)
                                                                                                                                   aaaGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGccc                   >  6:58808/1‑148 (MQ=255)
                                                                                                                                      gACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGACCGGTGCCTTGACTGTCAGCGAACTGGCAGCGGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAata               >  4:78760/1‑149 (MQ=255)
                                                                                                                                        gtcggCCCAGCCATAAGCCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCATGCGATCGACGATCACCAGACTCACAACGCCCAATATc             >  8:194893/4‑149 (MQ=255)
                                                                                                                                         aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTTCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCt            <  8:116298/149‑1 (MQ=255)
                                                                                                                                         aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAatat              >  4:225271/1‑147 (MQ=255)
                                                                                                                                         aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCt            <  8:187697/149‑1 (MQ=255)
                                                                                                                                         aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCt            >  5:250077/1‑149 (MQ=255)
                                                                                                                                         aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGACCGGTGCCTTGACTGTCAGCGAACTGGCAGCGGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCt            <  3:78760/149‑1 (MQ=255)
                                                                                                                                            gCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGc         <  3:211475/149‑1 (MQ=255)
                                                                                                                                              ccAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGccc       >  1:291840/1‑149 (MQ=255)
                                                                                                                                                   cTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGCCCGCGaa  <  3:225271/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTAAGGCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGCCCGCGAA  >  NC_002947/1523901‑1524196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑