Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,524,054 G→C 7.3% G253A (GGC→GCC)  murG → UDP‑N‑acetylglucosamine‑‑N‑acetylmuramyl‑ (pentapeptide) pyrophosphoryl‑undecaprenol N‑acetylglucosamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,524,0540GC 7.3% 236.6 / 4.9 76G253A (GGC→GCC) murGUDP‑N‑acetylglucosamine‑‑N‑acetylmuramyl‑ (pentapeptide) pyrophosphoryl‑undecaprenol N‑acetylglucosamine transferase
Reads supporting (aligned to +/- strand):  ref base G (31/39);  new base C (3/3);  total (34/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGCCCGCGAAG  >  NC_002947/1523906‑1524197
                                                                                                                                                    |                                                                                                                                                
gCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATgg                                                                                                                                                  >  5:155340/1‑149 (MQ=255)
  ccTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCt                                                                                                                                                >  8:42986/1‑149 (MQ=255)
     gTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGTCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTAGCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAATGAGATGGCCGAGGCTTATGGCTgg                                                                                                                                              >  7:108411/1‑148 (MQ=255)
     gTCCGAGGTGACCGCGGCGCTGCTTGCAGAGGGTTTCCACGAGGCGGGCAAGGAGCACGCACCGATCACCGCCGATCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTgg                                                                                                                                              <  8:108411/148‑1 (MQ=255)
      tCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTggg                                                                                                                                             <  7:22742/148‑1 (MQ=255)
      tCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑c                                                                                                                                           >  4:124775/1‑149 (MQ=255)
        cGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCGCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGCCATAAGCCTGGG‑cc                                                                                                                                          <  8:285489/148‑1 (MQ=255)
          aGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGAc                                                                                                                                       <  3:291003/149‑1 (MQ=255)
           ggTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGCCTGGG‑CCGAc                                                                                                                                       >  8:106755/1‑148 (MQ=255)
            gTGCCCGCTGCGCTGCGGCCAGAGGTGTTCCACCAGTCCGGCAAGCATCACGCACCGATCACCGCCGAGCTTTATCACGAGGCGGGCGTCGCGGCTCATGTAGAGCCTTTCATCAAATACATGGCCCAGCCATAAGCCTGGG‑ccatgtc                                                                                                                                     <  4:218165/149‑6 (MQ=255)
               cccGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCtggt                                                                                                                                  <  7:42986/149‑1 (MQ=255)
               cccGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCtgg                                                                                                                                   <  3:124775/148‑1 (MQ=255)
                ccGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCtggtg                                                                                                                                 <  1:137367/149‑1 (MQ=255)
                  gcggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTggtggt                                                                                                                               >  1:38383/1‑149 (MQ=255)
                   cggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGgtggtg                                                                                                                              >  7:26920/1‑149 (MQ=255)
                    ggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGgtgt                                                                                                                             <  6:310723/149‑1 (MQ=255)
                    ggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGgtgt                                                                                                                             <  7:216305/149‑1 (MQ=255)
                    ggcgCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGgtgt                                                                                                                             <  2:218341/149‑1 (MQ=255)
                         tGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTgccgg                                                                                                                        >  3:106159/1‑149 (MQ=255)
                             gCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACACGGCCCAGCCATAAGCCTGGG‑ccat                                                                                                                                        <  4:156618/129‑3 (MQ=255)
                             gCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACACGGCCCAGCCATAAGCCTGGG‑ccat                                                                                                                                        >  3:156618/1‑127 (MQ=255)
                              ccAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTgccgggccgg                                                                                                                   >  7:187697/1‑149 (MQ=255)
                                agagGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTg                                                                                                                 >  4:211475/1‑149 (MQ=255)
                                  agGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGcc                                                                                                               >  2:248581/1‑149 (MQ=255)
                                   ggTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCt                                                                                                              <  2:28888/149‑1 (MQ=255)
                                       ttCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTTGAGCCTTTCATCAATGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGAc                                                                                                          <  7:106755/149‑1 (MQ=255)
                                       ttCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGa                                                                                                           <  4:106159/148‑1 (MQ=255)
                                         ccaccaGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTg                                                                                                        >  3:146110/1‑149 (MQ=255)
                                         ccaccaGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTg                                                                                                        >  7:301628/1‑149 (MQ=255)
                                            ccaGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTct                                                                                                     >  1:293890/1‑148 (MQ=255)
                                                gCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCAGGGCCGGTGCCTTGACTGTCAGCg                                                                                                  >  2:17536/1‑148 (MQ=255)
                                                     cAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTgg                                                                                            <  4:146110/149‑1 (MQ=255)
                                                       agcagcACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGgca                                                                                          <  8:26920/149‑1 (MQ=255)
                                                            cacgcacCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGGCCCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGgcagcag                                                                                      <  1:248581/149‑1 (MQ=255)
                                                                cacCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGgc                                                                                 <  5:328/149‑1 (MQ=255)
                                                                cacCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGgc                                                                                 <  2:38383/149‑1 (MQ=255)
                                                                  ccGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCTGGTGCCTTGACTGTCAGCTAACTGGCAGCAGCAGgcct                                                                               <  3:229890/149‑1 (MQ=255)
                                                                   agATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGgcctg                                                                              >  2:27042/2‑149 (MQ=255)
                                                                        aCCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCtt                                                                          <  8:301628/148‑1 (MQ=255)
                                                                        aCCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTc                                                                         <  6:155340/149‑1 (MQ=255)
                                                                        aCCGCCGAGCGTTATCACGAGGCGGGCGTCGCGCCTCAGCTAGAGCCATTCATCAAAGACATGGCACAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCACCAGCAGGCCTGCCTTc                                                                         >  1:44550/1‑149 (MQ=255)
                                                                          cgccgAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGa                                                                       <  5:263556/149‑1 (MQ=255)
                                                                          cgccgAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGa                                                                       >  4:286245/1‑149 (MQ=255)
                                                                            ccgAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATg                                                                     >  1:123001/1‑149 (MQ=255)
                                                                                 cGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGt                                                                >  3:176056/1‑149 (MQ=255)
                                                                                          gAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGcc                                                       <  2:229708/149‑1 (MQ=255)
                                                                                            ggcgggcgTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGccc                                                      >  1:189066/1‑148 (MQ=255)
                                                                                               gggcgTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCAcg                                                  >  1:269807/1‑149 (MQ=255)
                                                                                                    tCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATc                                             <  2:189066/149‑1 (MQ=255)
                                                                                                    tCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATc                                             <  2:123001/149‑1 (MQ=255)
                                                                                                       cgGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCCGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATcgac                                          <  5:305757/149‑1 (MQ=255)
                                                                                                       cgGCTCAGCTAGATCCTTTCATCAAAGACATGGCCCCGGCTTATGGCTTGG‑CCGACCTGGGGTGGGGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATcgac                                          <  8:230624/149‑1 (MQ=255)
                                                                                                          cTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGGCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGAt                                       <  3:275376/149‑1 (MQ=255)
                                                                                                           tCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGAt                                       >  3:203699/1‑148 (MQ=255)
                                                                                                           tCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGAt                                       >  7:216146/1‑148 (MQ=255)
                                                                                                             aGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATc                                      <  6:9515/147‑1 (MQ=255)
                                                                                                              ggTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGNTGCCTTTGCCCCACGCGATCGACGATCAcc                                   <  1:27042/149‑1 (MQ=255)
                                                                                                                     ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGa                                               <  1:145072/130‑1 (MQ=255)
                                                                                                                     ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGa                                               >  2:145072/1‑130 (MQ=255)
                                                                                                                     ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATcga                                           <  1:169035/134‑1 (MQ=255)
                                                                                                                     ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATcga                                           >  2:169035/1‑134 (MQ=255)
                                                                                                                     ccTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTca                            <  8:216146/149‑1 (MQ=255)
                                                                                                                       tttCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTcac                           >  6:246274/1‑148 (MQ=255)
                                                                                                                           atcaAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTAGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGc                      <  6:240208/149‑1 (MQ=255)
                                                                                                                              aaaGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGccc                    >  6:58808/1‑148 (MQ=255)
                                                                                                                                 gACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGACCGGTGCCTTGACTGTCAGCGAACTGGCAGCGGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAata                >  4:78760/1‑149 (MQ=255)
                                                                                                                                   gtcggCCCAGCCATAAGCCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCATGCGATCGACGATCACCAGACTCACAACGCCCAATATc              >  8:194893/4‑149 (MQ=255)
                                                                                                                                    aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTTCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCt             <  8:116298/149‑1 (MQ=255)
                                                                                                                                    aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAatat               >  4:225271/1‑147 (MQ=255)
                                                                                                                                    aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCt             <  8:187697/149‑1 (MQ=255)
                                                                                                                                    aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCt             >  5:250077/1‑149 (MQ=255)
                                                                                                                                    aTGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGACCGGTGCCTTGACTGTCAGCGAACTGGCAGCGGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCt             <  3:78760/149‑1 (MQ=255)
                                                                                                                                       gCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGc          <  3:211475/149‑1 (MQ=255)
                                                                                                                                         ccAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGccc        >  1:291840/1‑149 (MQ=255)
                                                                                                                                              cTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGCCCGCGaa   <  3:225271/149‑1 (MQ=255)
                                                                                                                                                 attgCTGGT‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGCCCGCGAAg  <  5:236575/144‑1 (MQ=255)
                                                                                                                                                 aTGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTc                             >  4:306670/1‑120 (MQ=255)
                                                                                                                                                 aTGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTc                             <  3:306670/120‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GCCCTGTCCGAGGTGCCCGCGGCGCTGCGGCCAGAGGTGTTCCACCAGGCCGGCAAGCAGCACGCACCGATCACCGCCGAGCGTTATCACGAGGCGGGCGTCGCGGCTCAGGTAGAGCCTTTCATCAAAGACATGGCCCAGGCTTATGGCTGGG‑CCGACCTGGTGGTGTGCCGGGCCGGTGCCTTGACTGTCAGCGAACTGGCAGCAGCAGGCCTGCCTTCGATGCTGGTGCCTTTGCCCCACGCGATCGACGATCACCAGACTCACAACGCCCAATATCTGGCCCGCGAAG  >  NC_002947/1523906‑1524197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑