Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,536,251 A→G 7.1% intergenic (+42/‑114) argJ → / → PP_1347 glutamate N‑acetyltransferase/amino acid acetyltransferase/glutathione S‑transferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,536,2510AG 7.1% 153.9 / 3.0 56intergenic (+42/‑114)argJ/PP_1347glutamate N‑acetyltransferase/amino acid acetyltransferase/glutathione S‑transferase family protein
Reads supporting (aligned to +/- strand):  ref base A (27/25);  new base G (2/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01

GATCACCATCCGAATCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGATCATCGGCGACAAGCTGT  >  NC_002947/1536104‑1536398
                                                                                                                                                   |                                                                                                                                                   
gaTCACCATCCGAATCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCca                                                                                                                                                     >  5:101211/1‑148 (MQ=255)
 aTCACCATCCGAATCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCcaca                                                                                                                                                   <  2:62985/149‑1 (MQ=255)
  tCACCATCCGAATCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAg                                                                                                                                                  <  4:24126/149‑1 (MQ=255)
      cATCCGAATCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGgtgt                                                                                                                                              >  4:137561/1‑149 (MQ=255)
       aTCCGAATCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGtt                                                                                                                                             <  5:294594/149‑1 (MQ=255)
         ccGAATCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTg                                                                                                                                            >  1:167312/1‑148 (MQ=255)
           gAATCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCaa                                                                                                                                         >  8:286724/1‑149 (MQ=255)
           gAATCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCaa                                                                                                                                         >  1:16782/1‑149 (MQ=255)
            aaTCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAAt                                                                                                                                        <  1:127461/149‑1 (MQ=255)
            aaTCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAAt                                                                                                                                        <  3:137561/149‑1 (MQ=255)
            aaTCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCCCCCACAGgt                                                                                                                                                <  1:288172/141‑1 (MQ=255)
              tCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGc                                                                                                                                      >  5:227180/1‑149 (MQ=255)
              tCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGc                                                                                                                                      <  2:167312/149‑1 (MQ=255)
                  aCTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGc                                                                                                                                  <  7:141826/149‑1 (MQ=255)
                   cTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCTGCTCCCACAGGTGTTGCAATGCCTGCa                                                                                                                                 <  3:121907/149‑1 (MQ=255)
                             gCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCTGCAGGCATTGCAACACCTGTGGGAgcgggc                                                                                                                        <  1:271203/148‑3 (MQ=38)
                              ccAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAgcggtgcg                                                                                                                      <  7:16003/149‑1 (MQ=255)
                                    cAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCTGCAGGCATTGCAACACCTGTGGGAgcgggc                                                                                                                        >  4:237634/1‑139 (MQ=38)
                                    cAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCTGCAGGCATTGCAACACCTGTGGGAgcgggc                                                                                                                        <  3:237634/141‑3 (MQ=38)
                                     aGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGCTCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTg                                                                                                               >  4:99068/1‑149 (MQ=255)
                                       cGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTa                                                                                                             <  6:227180/149‑1 (MQ=255)
                                          aaCCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAg                                                                                                            >  4:299022/1‑147 (MQ=255)
                                          aaCCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAg                                                                                                            >  2:59525/1‑147 (MQ=255)
                                          aaCCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGa                                                                                                          <  3:171368/149‑1 (MQ=255)
                                                           aCCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGa                                                                                          >  7:187894/1‑148 (MQ=255)
                                                           aCCTGTCCCAAGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCAGCGa                                                                                          >  2:196845/1‑148 (MQ=255)
                                                            ccTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGaaa                                                                                        <  3:299022/149‑1 (MQ=255)
                                                             cTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAg                                                                                       >  2:39045/1‑149 (MQ=255)
                                                              tGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAgg                                                                                      >  8:219446/1‑149 (MQ=255)
                                                                   cACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCcgcg                                                                                 >  2:234268/1‑149 (MQ=255)
                                                                           cGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCg                                                                            >  1:267521/1‑146 (MQ=255)
                                                                           cGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCg                                                                            <  2:267521/146‑1 (MQ=255)
                                                                             tGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAgcggtg                                                                                                                        >  3:93640/1‑100 (MQ=255)
                                                                             tGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTg                                                                       >  2:37293/1‑149 (MQ=255)
                                                                                 gATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCTGCGCGGCCtttcgc                                                                   <  1:39045/149‑6 (MQ=255)
                                                                                      aCGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTg                                                              >  3:263045/1‑149 (MQ=255)
                                                                                        gCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGAGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGc                                                             >  1:303813/1‑148 (MQ=255)
                                                                                          cGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAg                                                          <  7:286724/149‑1 (MQ=255)
                                                                                           gAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGa                                                         <  3:268941/149‑1 (MQ=255)
                                                                                                  gtaccTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCt                                                  >  3:280555/1‑149 (MQ=255)
                                                                                                     ccTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGa                                                <  1:196845/148‑1 (MQ=255)
                                                                                                                 aTCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAAc                                     <  1:234268/147‑1 (MQ=255)
                                                                                                                   ccGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCAt                                  >  8:91859/1‑148 (MQ=255)
                                                                                                                    cGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGa                                <  7:136956/149‑1 (MQ=255)
                                                                                                                     gAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAg                               >  7:230510/1‑149 (MQ=255)
                                                                                                                         gCCTCTTCGCGGTCACGCCCGCTCCCACAGGTGTTGCAATGCCTGGAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTAt                           <  8:187894/149‑1 (MQ=255)
                                                                                                                                cgcgGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCt                      <  5:243345/147‑1 (MQ=255)
                                                                                                                                 gcgGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGat                   <  4:263045/149‑1 (MQ=255)
                                                                                                                                     gCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGatcatc               <  8:230510/149‑1 (MQ=255)
                                                                                                                                      cACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGATCATCg              >  7:218134/1‑149 (MQ=255)
                                                                                                                                       cgcaccGCTCCTGCAGGCATTGCAACACCTGTGGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGATCATCgg             >  7:62663/5‑149 (MQ=38)
                                                                                                                                       aCGCCCGCTCCCACAGGTGTTGGAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGATCATCgg             >  7:203093/1‑149 (MQ=255)
                                                                                                                                        cGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGATCATCGGc            <  7:91859/149‑1 (MQ=255)
                                                                                                                                         gCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGATCATCGGc            >  4:225278/1‑148 (MQ=255)
                                                                                                                                           ccGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGATCATCGGCGa          >  4:13759/1‑148 (MQ=255)
                                                                                                                                            cGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGATCATCGGCGAc         >  7:191000/1‑148 (MQ=255)
                                                                                                                                                 ccACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGATCATCGGCGACAAGCt    <  3:13759/148‑1 (MQ=255)
                                                                                                                                                 ccACAGGTGTTGCAATGCCTGCAGCAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGATCATCGGCGACAAGCTg   >  5:195508/1‑149 (MQ=255)
                                                                                                                                                  cacaGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGATCATCGGCGACAAGCTg   >  1:159286/1‑148 (MQ=255)
                                                                                                                                                   acaGGTGTTGCAATGCCTCCAGGACCGGAGCGCTCCCTGTAGGACCGGGCTTGCCAGCGAAAGCCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATCAGCGATCACCTGATCATCGCCGACAAGCTGt  >  5:192901/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATCACCATCCGAATCGAACTGGGCCGTGGCCAGTGCAGCGAAACCATCTGGACGACTGACCTGTCCCACGAGTACGTGAAGATCAACGCCGAGTACCGTACCTGATCTACTGATCCGAAGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTGTTGCAATGCCTGCAGGAGCGGTGCGGTCCCTGTAGGAGCGGGCTTGCCCGCGAAAGGCCGCGCAGCGGCCTGCGGGTTTTGCAAGACAAACCTGATGGAGCCGAACCATGAGCTATCACCTGATCATCGGCGACAAGCTGT  >  NC_002947/1536104‑1536398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑