Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 149,909 C→A 6.4% G347G (GGG→GGT)  PP_0142 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947149,9090CA 6.4% 198.3 / 3.5 62G347G (GGG→GGT) PP_0142ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (28/30);  new base A (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

GGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCG  >  NC_002947/149765‑150057
                                                                                                                                                |                                                                                                                                                    
ggcCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCa                                                                                                                                                  >  1:259602/1‑149 (MQ=255)
  ccGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCAcc                                                                                                                                                <  1:310104/149‑1 (MQ=255)
      aGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACt                                                                                                                                            <  2:259602/149‑1 (MQ=255)
         ggTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCgg                                                                                                                                         <  1:76764/149‑1 (MQ=255)
          gTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGTCGGTGGGGGCACACACCACCTCGgc                                                                                                                                        <  7:125171/149‑1 (MQ=255)
              cAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTg                                                                                                                                    >  7:199144/1‑149 (MQ=255)
                  tGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGc                                                                                                                                <  3:76959/149‑1 (MQ=255)
                     cgctGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCAGGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGa                                                                                                                             <  8:2833/149‑1 (MQ=255)
                      gcgGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCACCACCGACTCGGCGCTGCCGCTGAc                                                                                                                            >  1:164621/1‑149 (MQ=255)
                              cGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGaa                                                                                                                      <  7:276986/147‑1 (MQ=255)
                               gATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGcc                                                                                                                   >  5:299413/1‑149 (MQ=255)
                               gATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGcc                                                                                                                   >  4:63974/1‑149 (MQ=255)
                                    cGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGaa                                                                                                                      <  4:186214/141‑1 (MQ=255)
                                    cGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGaa                                                                                                                      >  3:186214/1‑141 (MQ=255)
                                    cGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCa                                                                                                              <  6:299413/149‑1 (MQ=255)
                                    cGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCa                                                                                                              <  5:729/149‑1 (MQ=255)
                                       aaTTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCa                                                                                                              >  5:250651/1‑146 (MQ=255)
                                            tGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGcc                                                                                                       >  8:41736/1‑148 (MQ=255)
                                              gCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGAt                                                                                                    <  7:263031/149‑1 (MQ=255)
                                                   tCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATggcgg                                                                                               >  2:57601/1‑149 (MQ=255)
                                                        aGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATc                                                                                          <  7:41736/149‑1 (MQ=255)
                                                        aGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATc                                                                                          <  1:57601/149‑1 (MQ=255)
                                                          cccATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAg                                                                                        <  7:176126/149‑1 (MQ=255)
                                                            cATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAgg                                                                                       >  3:259855/1‑148 (MQ=255)
                                                            cATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGa                                                                                      <  5:160683/149‑1 (MQ=255)
                                                             aTCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAgg                                                                                       >  4:192262/1‑147 (MQ=255)
                                                                 ccATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGAcc                                                                                                                           <  5:134445/107‑1 (MQ=255)
                                                                 ccATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGAcc                                                                                                                           >  6:134445/1‑107 (MQ=255)
                                                                  cATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCtt                                                                                                                 >  5:55949/1‑116 (MQ=255)
                                                                  cATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCtt                                                                                                                 <  6:55949/116‑1 (MQ=255)
                                                                   aTGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGCCTCGCCGCTGCCGGTGCCATTCAAGCCTTCCAGCCAGCCGATGGCGGCGAGCAGGAAGGCaa                                                                                >  7:188138/1‑148 (MQ=255)
                                                                      aagaaCAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGaa                                                                            >  5:79852/1‑149 (MQ=255)
                                                                       agaaCAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAAc                                                                           <  3:192262/149‑1 (MQ=255)
                                                                        gaaCAGCGCTGCCACCGCATCCAGCACGAGTACCACAAATATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACg                                                                          <  2:164621/149‑1 (MQ=255)
                                                                              cgcTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCt                                                                    >  3:265272/1‑149 (MQ=255)
                                                                              cgcTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCt                                                                    <  4:259855/149‑1 (MQ=255)
                                                                                   ccACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGc                                                                >  6:12269/1‑148 (MQ=255)
                                                                                       cGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGc                                                           <  4:243430/149‑1 (MQ=255)
                                                                                           tCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGt                                                                        <  5:96085/132‑1 (MQ=255)
                                                                                           tCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGt                                                                        >  6:96085/1‑132 (MQ=255)
                                                                                              aGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAg                                                    <  8:199144/149‑1 (MQ=255)
                                                                                                   gATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTg                                                                  <  1:133257/130‑1 (MQ=255)
                                                                                                   gATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTg                                                                  >  2:133257/1‑130 (MQ=255)
                                                                                                    aTGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGtg                                              <  6:79852/149‑1 (MQ=255)
                                                                                                     tGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGtgc                                             <  5:12269/149‑1 (MQ=255)
                                                                                                        ccgcaAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAg                                                                                    >  1:290095/4‑107 (MQ=255)
                                                                                                        ccgcaAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAg                                                                                    <  2:290095/104‑1 (MQ=255)
                                                                                                        ccACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGtgctgc                                          <  4:265272/149‑1 (MQ=255)
                                                                                                        ccACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGtgctg                                           >  2:229205/1‑148 (MQ=255)
                                                                                                          acaAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCAc                                        >  1:78959/1‑149 (MQ=255)
                                                                                                          acaAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCAc                                        >  6:240024/1‑149 (MQ=255)
                                                                                                                aTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAAt                                  <  7:220520/149‑1 (MQ=255)
                                                                                                                      tGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCt                            >  7:243189/1‑149 (MQ=255)
                                                                                                                           cagcgCCGAGTCGGTGGGGGCACACACCACCTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTg                         >  6:299810/5‑147 (MQ=38)
                                                                                                                             gcgCCGAGTCGGTGGGGGCACACACCACCTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTgcagc                     <  5:299810/147‑1 (MQ=255)
                                                                                                                                ccGAGTCGGTGGGGGCACACACCACCTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCagcagc                  >  8:223707/1‑149 (MQ=255)
                                                                                                                                ccGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTgcagca                    <  1:208127/147‑1 (MQ=255)
                                                                                                                                ccGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTgcagca                    >  2:208127/1‑147 (MQ=255)
                                                                                                                                  gAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCtt                           <  7:240313/138‑1 (MQ=255)
                                                                                                                                  gAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCtt                           >  8:240313/1‑138 (MQ=255)
                                                                                                                                   aGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGa                >  4:405/1‑148 (MQ=255)
                                                                                                                                              gCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGACAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGc    >  8:192161/1‑149 (MQ=255)
                                                                                                                                                ccccACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCg  >  4:263637/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GGCCGAAGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCG  >  NC_002947/149765‑150057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑