Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,541,267 C→T 8.0% A86A (GCG→GCA)  panE ← 2‑dehydropantoate 2‑reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,541,2670CT 8.0% 249.1 / 6.3 86A86A (GCG→GCA) panE2‑dehydropantoate 2‑reductase
Reads supporting (aligned to +/- strand):  ref base C (37/42);  new base T (2/5);  total (39/47)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCTCGCGGAAGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAGGCCTG  >  NC_002947/1541120‑1541410
                                                                                                                                                   |                                                                                                                                               
ccccTCGCGGAAGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATc                                                                                                                                                 <  6:141538/148‑1 (MQ=255)
ccccTCGCGGAAGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCg                                                                                                                                                <  2:320654/149‑1 (MQ=255)
  ccTCGCGGAAGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGcc                                                                                                                                              >  8:47502/1‑149 (MQ=255)
  ccTCGCGGAAGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGcc                                                                                                                                              >  5:291667/1‑149 (MQ=255)
   cTCGCGGAAGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCg                                                                                                                                             >  8:111485/1‑149 (MQ=255)
   cTCGCGGAAGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCg                                                                                                                                             >  8:106850/1‑149 (MQ=255)
        ggAAGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCGATTGCCggtgta                                                                                                                                        <  1:277812/149‑5 (MQ=255)
          aaGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTGCCCCGGCGATTGCCGGTGTAGc                                                                                                                                      <  5:171832/149‑1 (MQ=255)
          aaGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGc                                                                                                                                      <  3:100517/149‑1 (MQ=255)
          aaGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGc                                                                                                                                      >  7:115021/1‑149 (MQ=255)
            ggCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCGTGGCTGCCCAGGACATTCTGCAACAGCATCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTGCCCCGGCGATTGCCGGTGTAGCgc                                                                                                                                    <  4:199151/149‑2 (MQ=255)
            ggCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGt                                                                                                                                    >  1:141299/1‑149 (MQ=255)
            ggCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGt                                                                                                                                    <  6:291667/149‑1 (MQ=255)
              cgcCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCa                                                                                                                                  >  4:281410/1‑149 (MQ=255)
                 cTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCAGTTCGGCGCTCTGGGCCAGCCGGGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAg                                                                                                                               <  5:178078/149‑1 (MQ=255)
                 cTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAg                                                                                                                               <  5:12986/149‑1 (MQ=255)
                 cTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAg                                                                                                                               <  3:145703/149‑1 (MQ=255)
                 cTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGAGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAg                                                                                                                               >  8:286969/1‑149 (MQ=255)
                     ggTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCt                                                                                                                           <  7:286969/149‑1 (MQ=255)
                     ggTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCt                                                                                                                           >  4:48912/1‑149 (MQ=255)
                     ggTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCt                                                                                                                           <  7:12603/149‑1 (MQ=255)
                       tGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTg                                                                                                                         >  3:106506/1‑149 (MQ=255)
                          tGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAg                                                                                                                      >  3:17127/1‑149 (MQ=255)
                            gAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAg                                                                                                                      <  6:112273/147‑1 (MQ=255)
                              ggCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGcca                                                                                                                  <  6:13811/149‑1 (MQ=255)
                                cGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGccacc                                                                                                                <  8:258549/149‑1 (MQ=255)
                                 gAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTACCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGccac                                                                                                                 >  4:127979/1‑147 (MQ=255)
                                  aaGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACcag                                                                                                              <  6:257167/149‑1 (MQ=255)
                                   aGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACcagc                                                                                                             <  8:75975/149‑1 (MQ=255)
                                       gCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGcc                                                                                                         >  2:138520/1‑149 (MQ=255)
                                        cAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCg                                                                                                        <  4:100025/149‑1 (MQ=255)
                                         agcgagcgTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCgg                                                                                                       <  8:287588/149‑1 (MQ=255)
                                          gcgagcgTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGt                                                                                                      <  3:48912/149‑1 (MQ=255)
                                                 tGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCgg                                                                                               >  6:188927/1‑149 (MQ=255)
                                                    ggCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGcc                                                                                            <  4:106506/149‑1 (MQ=255)
                                                    ggCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGcc                                                                                            >  2:249214/1‑149 (MQ=255)
                                                       aCCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTg                                                                                         >  5:215529/1‑149 (MQ=255)
                                                        ccAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTgc                                                                                        <  5:188927/149‑1 (MQ=255)
                                                          aGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTgcgc                                                                                      <  8:115021/149‑1 (MQ=255)
                                                            gTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCtt                                                                                    <  3:281410/149‑1 (MQ=255)
                                                             tCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTtg                                                                                   >  4:309425/1‑149 (MQ=255)
                                                               ggCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTtgt                                                                                  <  1:138520/148‑1 (MQ=255)
                                                               ggCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTtgt                                                                                  <  7:201165/148‑1 (MQ=255)
                                                                  gACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCg                                                                              >  1:280814/1‑149 (MQ=255)
                                                                     ttCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGttt                                                                           >  2:99979/1‑149 (MQ=255)
                                                                        aTCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATTGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCg                                                                         <  8:138913/148‑1 (MQ=255)
                                                                              gCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCgg                                                                    <  6:102038/147‑1 (MQ=255)
                                                                                  cccAGGCCATCCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATg                                                                <  1:55205/147‑1 (MQ=255)
                                                                                    cAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCg                                                             >  5:145384/1‑148 (MQ=255)
                                                                                    cAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCg                                                             >  4:61719/1‑148 (MQ=255)
                                                                                     aGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTa                                                           <  3:211502/149‑1 (MQ=255)
                                                                                         cATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAgcgc                                                       >  7:96470/1‑149 (MQ=255)
                                                                                         cATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAgcgc                                                       >  4:287909/1‑149 (MQ=255)
                                                                                                cAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGtt                                                 <  4:17127/148‑1 (MQ=255)
                                                                                                cAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGtt                                                 <  4:118765/148‑1 (MQ=255)
                                                                                                cAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGGGGCGCTACACCGGCAATCGCAGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGTCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGttt                                                <  2:308539/149‑1 (MQ=255)
                                                                                                 aaCAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTg                                               <  1:249214/149‑1 (MQ=255)
                                                                                                  aCAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTgg                                              >  2:290394/1‑149 (MQ=255)
                                                                                                    agcagcACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTc                                            <  1:267706/149‑1 (MQ=255)
                                                                                                    agcagcACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTc                                            <  3:287909/149‑1 (MQ=255)
                                                                                                     gcagcaCTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTcc                                           <  7:111485/149‑1 (MQ=255)
                                                                                                     gcagcaCTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTcc                                           <  7:106850/149‑1 (MQ=255)
                                                                                                       agcaCTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTACGGCGTAGCGCCGGGTTTGGTCCTg                                         >  8:93278/1‑149 (MQ=255)
                                                                                                          aCTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTc                                      <  2:141299/149‑1 (MQ=255)
                                                                                                            ttCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGa                                    >  7:106153/1‑149 (MQ=255)
                                                                                                              cGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGAc                                   >  7:68816/1‑148 (MQ=255)
                                                                                                                gcgcTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAg                                >  2:188617/1‑149 (MQ=255)
                                                                                                                   ctctCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGt                             >  6:328681/1‑149 (MQ=255)
                                                                                                                     ctcGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGt                             >  8:25170/1‑147 (MQ=255)
                                                                                                                      tcGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAg                          >  6:231339/1‑149 (MQ=255)
                                                                                                                        ggCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCa                           <  3:309425/146‑1 (MQ=255)
                                                                                                                         gCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCgg                                                    <  4:272735/120‑1 (MQ=255)
                                                                                                                         gCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCgg                                                    >  3:272735/1‑120 (MQ=255)
                                                                                                                         gCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGcc                       <  1:188617/149‑1 (MQ=255)
                                                                                                                            aGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCAcc                    >  6:127126/1‑149 (MQ=255)
                                                                                                                            aGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCAcc                    >  3:63635/1‑149 (MQ=255)
                                                                                                                             gCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCg                   <  2:280814/149‑1 (MQ=255)
                                                                                                                              ccGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCgg                  >  4:280259/1‑149 (MQ=255)
                                                                                                                               tatgacgcTGCCCCGGCGATTGCCGGTGTAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGc                 >  6:296665/6‑149 (MQ=255)
                                                                                                                               tatgacgcTGCCCCGGCGATTGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGc                 >  4:262323/6‑149 (MQ=255)
                                                                                                                                     gcTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCctgct            >  1:177536/1‑148 (MQ=255)
                                                                                                                                         ccccGGCGATTGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGAt         <  3:262323/145‑1 (MQ=255)
                                                                                                                                         cacCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGTCCTGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCATGGTCAGGCCACCGGCCTGCTGAt         <  7:47502/147‑1 (MQ=255)
                                                                                                                                         cacCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATa        <  6:145384/148‑1 (MQ=255)
                                                                                                                                         cacCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAg       <  5:328681/149‑1 (MQ=255)
                                                                                                                                           ccGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAGGc     >  4:126687/1‑149 (MQ=255)
                                                                                                                                              gCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAGGCCTg  <  3:197989/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CCCCTCGCGGAAGGCGCCTTCGGTGCTGGAGGCGAAGATGCAGCGAGCGTGAGGCACCAGGTCGGCGACTTCATCCTGGCTGCCCAGGCCATTCTGCAACAGCAGCACTTCGGCGCTCTCGGCCAGCCGTGGCGCTACACCGGCAATCGCCGGGGCAGCGTCATAGGCCTTGCAGGCCACCAGCAGCCGGTGGATCGGGCCTTGCGCTTGTGCCGTTTCGGCCGGTATGGCGTAGCGCCGGGTTTGGTCCTGCTCGACCAGGGTCAGGCCACCGGCCTGCTGATAGGCCTG  >  NC_002947/1541120‑1541410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑