| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,563,817 | A→G | 7.1% | intergenic (+83/+39) | PP_1372 → / ← pitB | hypothetical protein/phosphate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,563,817 | 0 | A | G | 7.1% | 156.5 / 2.6 | 56 | intergenic (+83/+39) | PP_1372/pitB | hypothetical protein/phosphate transporter |
| Reads supporting (aligned to +/- strand): ref base A (28/24); new base G (2/2); total (30/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
GTTGGGGCGGCAGTTGGTGCGCGGGTTGATGGGG‑T‑CGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGC > NC_002947/1563674‑1563964 | gTTGGGGCGGCAGTTGGTGCGCGGGTTGATGGGG‑T‑CGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑Ggc > 4:303676/1‑149 (MQ=255) ggggCGGCAGTTGGTGCGCGGGTTGATGGGGAG‑CGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGAACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCAATGAA‑AAA‑Ggcgc > 1:204653/1‑149 (MQ=255) ggggCGGCAGTTGGTGCGCGGGTTGATGGGG‑TACGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAATCCCGCTCCGGCAAAGAACAAGGTGGCGAAGGCATAACGGCTATGAA‑AAA‑Ggcgc > 1:200043/1‑149 (MQ=255) gcggcAGTTGGTGCGCGGGTTGATGGGG‑T‑CGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGccc > 7:184931/1‑147 (MQ=255) gTTGGTGCGCGGGTTGATGGGG‑T‑CGTTGTTAGGGGGCAAGAAAAGGTAGTCATCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGAGACCGAGGgcgc > 1:243783/1‑149 (MQ=255) ttGGTGCGCGGGTTGATGGGG‑T‑CGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGcc > 8:5377/1‑149 (MQ=255) ttgTGCGCGGGTTGATGGGG‑T‑CGTTGTTAGGTGGCAAGAAAAGGTATTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGcc < 2:204653/146‑1 (MQ=255) tGGTGCGCGGGTTGATGGGG‑T‑CGTTTTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTTGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGcc < 2:200043/148‑1 (MQ=255) gTGCGCGGGTTGATGGGG‑T‑CGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑GAA‑GGCGCCCTCGGGCGCCttt > 6:7013/1‑148 (MQ=255) cgcgGGTTGATGGGG‑T‑CGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCGCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑GAA‑GGCGCCCTCGGGCGCCtttttc < 5:7013/149‑5 (MQ=255) aTGGGG‑T‑CGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGgt < 4:282954/149‑1 (MQ=255) ggg‑T‑CGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGgtgt > 1:29338/1‑148 (MQ=255) ttgttAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCttca > 4:220664/1‑129 (MQ=255) ttgttAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCCCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCttca < 3:220664/129‑1 (MQ=255) aGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAATGAA‑GGCGccctc > 8:204847/1‑112 (MQ=255) aGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAATGAA‑GGCGccctc < 7:204847/114‑3 (MQ=255) aGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGAGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAAt > 2:206118/1‑149 (MQ=255) cAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCttt < 4:220915/149‑1 (MQ=255) aGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTgg < 8:184931/149‑1 (MQ=255) ggTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACgcca < 7:5377/149‑1 (MQ=255) gTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACgccag > 5:323618/1‑149 (MQ=255) cTGCGCCGCTCCATACCCATGGTAGGACAAGCCCGCTCCGTCAAAGAACAAGTTGGCGTAGTCATTACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGATTTGGACGCCAGCCAGAAc < 4:244263/147‑1 (MQ=255) tGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAgg > 4:125246/1‑149 (MQ=255) gcgccgcgccATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAggc > 5:53119/1‑149 (MQ=255) gccgcgccATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAggccg < 2:29338/149‑1 (MQ=255) ccgcgccATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAggccgg > 5:294792/1‑149 (MQ=255) gccATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGgccgc > 5:176515/1‑149 (MQ=255) aTACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAg > 4:181406/1‑149 (MQ=255) tACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAgg < 3:303676/149‑1 (MQ=255) ccTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGccagccag < 2:90060/128‑1 (MQ=255) ccTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGccagccag > 1:90060/1‑128 (MQ=255) ccTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGcc > 5:290263/1‑148 (MQ=255) ttGATAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCAt > 5:213590/1‑149 (MQ=255) gTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCg < 3:156615/148‑1 (MQ=255) gTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCg < 6:294792/148‑1 (MQ=255) tAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGag > 8:104344/1‑149 (MQ=255) gCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGaggc < 6:323618/149‑1 (MQ=255) cTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCa < 6:176515/149‑1 (MQ=255) cGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAAGGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAg > 1:226066/1‑148 (MQ=255) cGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGa > 8:185031/1‑149 (MQ=255) cGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGa < 1:206118/149‑1 (MQ=255) cAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGAcc > 4:195895/1‑148 (MQ=255) cAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGAcc < 3:195895/148‑1 (MQ=255) aaaGAACACGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAggccg < 4:6961/111‑1 (MQ=255) aaaGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGACCCa > 8:150701/1‑149 (MQ=255) aacaaCAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGACCCAt < 7:104344/146‑1 (MQ=255) aaCAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGACCCATGc > 7:267277/1‑148 (MQ=255) aCAAGGTGGCGTAGGCATAACGGCTATGAA‑ACA‑GGCGCCCGAGGGCTCCTTCATTCATTGCGGGGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCATGGGTCCTGAccctgcct < 6:182061/149‑6 (MQ=255) aCAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGACCCATGc > 6:210898/1‑147 (MQ=255) gCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGACCCATGCCAGCAGGAt < 2:226066/148‑1 (MQ=255) gTAGGAATAACGGATTTGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGACCCATGCCAGCAGGATGGt < 6:213590/149‑1 (MQ=255) gCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGACCCATGCCAGCAGGATGGtttt > 1:205294/1‑148 (MQ=255) gCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGACCCATGCCAGCAGGATGGTTTTc < 7:185031/149‑1 (MQ=255) tAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGACCCATGCCAGCAGGATGGTTTTca > 8:85193/1‑147 (MQ=255) cGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGAcc < 4:89775/122‑1 (MQ=255) cGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGAcc > 3:89775/1‑122 (MQ=255) a‑aaa‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGc < 7:150701/149‑1 (MQ=255) | GTTGGGGCGGCAGTTGGTGCGCGGGTTGATGGGG‑T‑CGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAA‑AAA‑GGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCA‑GGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGC > NC_002947/1563674‑1563964 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |