Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 149,917 A→C 7.8% S345A (TCG→GCG)  PP_0142 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947149,9170AC 7.8% 183.6 / 4.9 61S345A (TCG→GCG) PP_0142ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (27/29);  new base C (3/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

AGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCG  >  NC_002947/149771‑150057
                                                                                                                                                  |                                                                                                                                            
aGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACt                                                                                                                                            <  2:259602/149‑1 (MQ=255)
   ggTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCgg                                                                                                                                         <  1:76764/149‑1 (MQ=255)
    gTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGTCGGTGGGGGCACACACCACCTCGgc                                                                                                                                        <  7:125171/149‑1 (MQ=255)
        cAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTg                                                                                                                                    >  7:199144/1‑149 (MQ=255)
            tGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGc                                                                                                                                <  3:76959/149‑1 (MQ=255)
               cgctGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCAGGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGa                                                                                                                             <  8:2833/149‑1 (MQ=255)
                gcgGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCACCACCGACTCGGCGCTGCCGCTGAc                                                                                                                            >  1:164621/1‑149 (MQ=255)
                        cGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGaa                                                                                                                      <  7:276986/147‑1 (MQ=255)
                         gATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGcc                                                                                                                   >  5:299413/1‑149 (MQ=255)
                         gATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGcc                                                                                                                   >  4:63974/1‑149 (MQ=255)
                              cGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGaa                                                                                                                      <  4:186214/141‑1 (MQ=255)
                              cGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGaa                                                                                                                      >  3:186214/1‑141 (MQ=255)
                              cGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCa                                                                                                              <  6:299413/149‑1 (MQ=255)
                              cGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCa                                                                                                              <  5:729/149‑1 (MQ=255)
                                 aaTTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCa                                                                                                              >  5:250651/1‑146 (MQ=255)
                                      tGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGcc                                                                                                       >  8:41736/1‑148 (MQ=255)
                                        gCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGAt                                                                                                    <  7:263031/149‑1 (MQ=255)
                                             tCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATggcgg                                                                                               >  2:57601/1‑149 (MQ=255)
                                                  aGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATc                                                                                          <  7:41736/149‑1 (MQ=255)
                                                  aGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATc                                                                                          <  1:57601/149‑1 (MQ=255)
                                                    cccATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAg                                                                                        <  7:176126/149‑1 (MQ=255)
                                                      cATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAgg                                                                                       >  3:259855/1‑148 (MQ=255)
                                                      cATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGa                                                                                      <  5:160683/149‑1 (MQ=255)
                                                       aTCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAgg                                                                                       >  4:192262/1‑147 (MQ=255)
                                                           ccATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGAcc                                                                                                                           <  5:134445/107‑1 (MQ=255)
                                                           ccATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGAcc                                                                                                                           >  6:134445/1‑107 (MQ=255)
                                                            cATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCtt                                                                                                                 <  6:55949/116‑1 (MQ=255)
                                                            cATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCtt                                                                                                                 >  5:55949/1‑116 (MQ=255)
                                                             aTGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGCCTCGCCGCTGCCGGTGCCATTCAAGCCTTCCAGCCAGCCGATGGCGGCGAGCAGGAAGGCaa                                                                                >  7:188138/1‑148 (MQ=255)
                                                                aagaaCAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGaa                                                                            >  5:79852/1‑149 (MQ=255)
                                                                 agaaCAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAAc                                                                           <  3:192262/149‑1 (MQ=255)
                                                                  gaaCAGCGCTGCCACCGCATCCAGCACGAGTACCACAAATATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACg                                                                          <  2:164621/149‑1 (MQ=255)
                                                                        cgcTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCt                                                                    <  4:259855/149‑1 (MQ=255)
                                                                        cgcTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCt                                                                    >  3:265272/1‑149 (MQ=255)
                                                                             ccACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGc                                                                >  6:12269/1‑148 (MQ=255)
                                                                                 cGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGc                                                           <  4:243430/149‑1 (MQ=255)
                                                                                     tCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGt                                                                        <  5:96085/132‑1 (MQ=255)
                                                                                     tCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGt                                                                        >  6:96085/1‑132 (MQ=255)
                                                                                        aGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAg                                                    <  8:199144/149‑1 (MQ=255)
                                                                                             gATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTg                                                                  <  1:133257/130‑1 (MQ=255)
                                                                                             gATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTg                                                                  >  2:133257/1‑130 (MQ=255)
                                                                                              aTGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGtg                                              <  6:79852/149‑1 (MQ=255)
                                                                                               tGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGtgc                                             <  5:12269/149‑1 (MQ=255)
                                                                                                  ccgcaAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAg                                                                                    >  1:290095/4‑107 (MQ=255)
                                                                                                  ccgcaAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAg                                                                                    <  2:290095/104‑1 (MQ=255)
                                                                                                  ccACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGtgctgc                                          <  4:265272/149‑1 (MQ=255)
                                                                                                  ccACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGtgctg                                           >  2:229205/1‑148 (MQ=255)
                                                                                                    acaAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCAc                                        >  1:78959/1‑149 (MQ=255)
                                                                                                    acaAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCAc                                        >  6:240024/1‑149 (MQ=255)
                                                                                                          aTCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAAt                                  <  7:220520/149‑1 (MQ=255)
                                                                                                                tGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCt                            >  7:243189/1‑149 (MQ=255)
                                                                                                                     cagcgCCGAGTCGGTGGGGGCACACACCACCTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTg                         >  6:299810/5‑147 (MQ=38)
                                                                                                                       gcgCCGAGTCGGTGGGGGCACACACCACCTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTgcagc                     <  5:299810/147‑1 (MQ=255)
                                                                                                                          ccGAGTCGGTGGGGGCACACACCACCTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCagcagc                  >  8:223707/1‑149 (MQ=255)
                                                                                                                          ccGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTgcagca                    >  2:208127/1‑147 (MQ=255)
                                                                                                                          ccGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTgcagca                    <  1:208127/147‑1 (MQ=255)
                                                                                                                            gAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCtt                           <  7:240313/138‑1 (MQ=255)
                                                                                                                            gAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCtt                           >  8:240313/1‑138 (MQ=255)
                                                                                                                             aGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGa                >  4:405/1‑148 (MQ=255)
                                                                                                                                        gCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGACAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGc    >  8:192161/1‑149 (MQ=255)
                                                                                                                                          ccccACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCg  >  4:263637/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
AGCGGTTGCAGATGCCGCGGGCTTCGATTACGCAATTCTGGCCACTCACCAGCCCATCTCCATGAAGAACAGCGCTGCCACCGCATCCAGCACGATGACCACAAAAATCGACTGCACCACTGCCGAGGTGGTGTGTGCCCCCACCGACTCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCG  >  NC_002947/149771‑150057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑