Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,581,266 G→T 100% A44D (GCC→GAC)  ttgB ← efflux pump membrane protein TtgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,581,2660GT100.0% 164.7 / NA 50A44D (GCC→GAC) ttgBefflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/27);  total (23/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGGTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATGGTT  >  NC_002947/1581122‑1581400
                                                                                                                                                |                                                                                                                                      
gCGGTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACggcgatgtcgat                                                                                                                                    >  7:274326/1‑149 (MQ=255)
gCGGTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGACCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACggcgatgtcgat                                                                                                                                    >  2:38265/1‑149 (MQ=255)
    tAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGAtggct                                                                                                                                >  5:114662/1‑149 (MQ=255)
    tAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGAtggct                                                                                                                                <  3:81953/149‑1 (MQ=255)
        ggTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACTACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGAtggctggag                                                                                                                            >  4:211421/1‑147 (MQ=255)
            aTGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGgcgc                                                                                                                        <  1:111662/149‑1 (MQ=255)
                tgcCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATa                                                                                                                    >  6:238794/1‑149 (MQ=255)
                    gTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACggcgatgtcga                                                                                                                                     >  8:89431/1‑128 (MQ=255)
                    gTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACggcgatgtcga                                                                                                                                     <  7:89431/128‑1 (MQ=255)
                     tCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTggg                                                                                                               <  8:274326/149‑1 (MQ=255)
                     tCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTggg                                                                                                               <  3:196449/149‑1 (MQ=255)
                         aGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTAc                                                                                                           <  5:238794/149‑1 (MQ=255)
                           ttACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGGGCGATACTGGGGTACTg                                                                                                         <  3:206531/149‑1 (MQ=255)
                             aCTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTgg                                                                                                        <  4:44827/148‑1 (MQ=255)
                                     acgacACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGAt                                                                                                   >  5:85301/1‑145 (MQ=255)
                                     acgacACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGAt                                                                                                   <  6:85301/145‑1 (MQ=255)
                                       gacACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGACGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAg                                                                                             >  2:276872/1‑149 (MQ=255)
                                          acaTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGt                                                                                                       >  3:302577/1‑136 (MQ=255)
                                          acaTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGt                                                                                                       <  4:302577/136‑1 (MQ=255)
                                            aTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTATTGGTTGATCGGCAGCTTCa                                                                                        <  7:181237/149‑1 (MQ=255)
                                            aTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCtt                                                                                          >  6:140077/1‑147 (MQ=255)
                                              aaCGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGAtggct                                                                                                                                <  5:298365/107‑1 (MQ=255)
                                              aaCGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGAtggct                                                                                                                                >  6:298365/1‑107 (MQ=255)
                                                      ttGTCGATACCGTTGAGCTGCTGCTCGACCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACaa                                                                              <  1:38265/149‑1 (MQ=255)
                                                           gATACCGTTGTGGTGCTTCTCGATCTCCTGCACGACGGTGTCCTGCACGGTTTGCTCCGAGGCGCCCGGGTAGGTCTCGGCGATTTCGATGGGTGGCGTCGCGTTACTGGGGTACTGGTTGATCGGCAGCTTCAGGAGTGACAAGGccg                                                                         <  4:116431/149‑2 (MQ=255)
                                                           gATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGccc                                                                         >  8:299910/1‑149 (MQ=255)
                                                            aTACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGcc                                                                          <  8:107667/147‑1 (MQ=255)
                                                            aTACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGcc                                                                          >  7:107667/1‑147 (MQ=255)
                                                               ccGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGAc                                                                     <  2:84488/149‑1 (MQ=255)
                                                                 gTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCa                                                                   <  4:311235/149‑1 (MQ=255)
                                                                 gTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCa                                                                   <  2:325666/149‑1 (MQ=255)
                                                                   tGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGAt                                                                                    >  5:220866/1‑130 (MQ=255)
                                                                   tGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGAt                                                                                    <  6:220866/130‑1 (MQ=255)
                                                                     aGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCa                                                                <  6:114662/148‑1 (MQ=255)
                                                                            gctCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCAc                                                         >  5:68399/1‑148 (MQ=255)
                                                                             ctCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCAcc                                                        <  7:299910/148‑1 (MQ=255)
                                                                                gATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAgg                                                    >  5:158132/1‑149 (MQ=255)
                                                                                          accacGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCAcc                                            >  7:34111/1‑147 (MQ=255)
                                                                                           ccacGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCACGACCAGCATGATCACCAAGGCGATCACCCAg                                         >  5:209239/1‑149 (MQ=255)
                                                                                            cacGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAgg                                        >  6:285692/1‑149 (MQ=255)
                                                                                                     cTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCa                                              <  1:232851/134‑1 (MQ=255)
                                                                                                     cTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCa                                              >  2:232851/1‑134 (MQ=255)
                                                                                                     cTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCg                                      >  1:289638/1‑142 (MQ=255)
                                                                                                      tGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGaa                                    >  4:313351/1‑143 (MQ=255)
                                                                                                      tGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGaa                                    <  3:313351/143‑1 (MQ=255)
                                                                                                           ggTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAg                                                                  >  4:248390/1‑108 (MQ=255)
                                                                                                           ggTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAg                                                                  <  3:248390/108‑1 (MQ=255)
                                                                                                                gcgcCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGGCAAGGc                                                                           >  2:79676/1‑94 (MQ=255)
                                                                                                                gcgcCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGc                                                                           <  1:79676/94‑1 (MQ=255)
                                                                                                                  gcCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATa                    >  3:317860/1‑147 (MQ=255)
                                                                                                                   ccGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAg                 <  5:140077/149‑1 (MQ=255)
                                                                                                                          gcCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTc           >  2:301483/1‑148 (MQ=255)
                                                                                                                                gTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATgg    <  1:301483/149‑1 (MQ=255)
                                                                                                                                   ggTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCGGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATGGtt  <  3:211421/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                      
GCGGTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATGGTT  >  NC_002947/1581122‑1581400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑