Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,585,220 G→C 9.8% intergenic (‑207/‑169) PP_1388 ← / → PP_1389 EmrB/QacA family drug resistance transporter/oxaloacetate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,585,2200GC 9.8% 150.8 / 5.7 50intergenic (‑207/‑169)PP_1388/PP_1389EmrB/QacA family drug resistance transporter/oxaloacetate decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (25/20);  new base C (3/2);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCGGTAGTTA  >  NC_002947/1585072‑1585361
                                                                                                                                                    |                                                                                                                                             
cccTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAgg                                                                                                                                               <  3:155271/149‑1 (MQ=255)
         tGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTgca                                                                                                                                       <  7:90235/148‑1 (MQ=255)
         tGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATACAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTgcag                                                                                                                                      >  5:165447/1‑149 (MQ=255)
           tAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTgcagc                                                                                                                                     <  4:226305/148‑1 (MQ=255)
           tAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAACCTGGTgcaagc                                                                                                                                    <  4:168905/149‑4 (MQ=255)
                tGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTg                                                                                                                               >  7:133636/1‑149 (MQ=255)
                   gTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCg                                                                                                                            >  5:248122/1‑149 (MQ=255)
                       ggTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAg                                                                                                                         <  1:318284/148‑1 (MQ=255)
                            cTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGttttt                                                                                                                   <  6:294942/149‑1 (MQ=255)
                            cTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGttttt                                                                                                                   >  4:236956/1‑149 (MQ=255)
                            cTTCCTATGTACTATCTGCATCCCCTAGGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCAGGGGCTTGCACCAGGTGGGTGCAGCAGATTGCGGCAGGgtttt                                                                                                                   >  8:224168/1‑149 (MQ=255)
                                        tATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCAT‑GCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGtct                                                                                                      >  7:38655/1‑149 (MQ=255)
                                         aTCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGtct                                                                                                      >  5:92216/1‑149 (MQ=255)
                                          tCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGtctc                                                                                                     >  1:319127/1‑149 (MQ=255)
                                             gCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCca                                                                                                  >  2:112401/1‑149 (MQ=255)
                                              cATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGtctc                                                                                                     <  4:101055/145‑1 (MQ=255)
                                                   ccTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCtt                                                                                            <  6:92216/149‑1 (MQ=255)
                                                    cTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCttt                                                                                           <  2:319127/149‑1 (MQ=255)
                                                       tGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTAt                                                                                        >  2:152916/1‑149 (MQ=255)
                                                        gCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTAtt                                                                                       >  5:281285/1‑149 (MQ=255)
                                                        gCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTAtt                                                                                       >  5:276037/1‑149 (MQ=255)
                                                        gCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTAt                                                                                        >  6:186160/1‑148 (MQ=255)
                                                             aTCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCctct                                                                                  >  6:280866/1‑149 (MQ=255)
                                                                                cccAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGa                                                               >  6:197182/2‑149 (MQ=255)
                                                                                  cAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGAc                                                              <  6:281285/148‑1 (MQ=255)
                                                                                  cAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGAc                                                              <  6:276037/148‑1 (MQ=255)
                                                                                        tgcATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGcc                                                          >  4:166095/1‑146 (MQ=255)
                                                                                              gCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGc                                                  >  5:313937/1‑148 (MQ=255)
                                                                                                  cGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACgg                                             <  6:248122/149‑1 (MQ=255)
                                                                                                     gATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCAAGACCGCCCAGATGGCGACGGTTg                                          >  4:177887/1‑149 (MQ=255)
                                                                                                         tCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTCTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGaa                                       <  8:133636/148‑1 (MQ=255)
                                                                                                              ggTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAAcaggt                                  <  3:166095/148‑1 (MQ=255)
                                                                                                                tATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAAcaggtcag                               <  7:224168/149‑1 (MQ=255)
                                                                                                                    cctcaGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAAcaggtcagg                              >  8:92468/4‑146 (MQ=255)
                                                                                                                      acaGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGAc                         <  5:280866/149‑1 (MQ=255)
                                                                                                                              tGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTa                  >  4:278000/1‑148 (MQ=255)
                                                                                                                              tGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTa                  >  4:134611/1‑148 (MQ=255)
                                                                                                                                 aaTCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAgg              <  8:38655/149‑1 (MQ=255)
                                                                                                                                     cGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGt           <  1:152916/148‑1 (MQ=255)
                                                                                                                                     cGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTc          >  1:326040/1‑149 (MQ=255)
                                                                                                                                      ggggCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCCCACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCg         >  4:302369/1‑149 (MQ=255)
                                                                                                                                      ggggCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGaa                                       >  6:70726/1‑119 (MQ=255)
                                                                                                                                      ggggCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGaa                                       <  5:70726/119‑1 (MQ=255)
                                                                                                                                         gCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCGGTa      <  5:186160/149‑1 (MQ=255)
                                                                                                                                          gctGCACCAACCTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAAcag                                    <  4:40830/116‑1 (MQ=255)
                                                                                                                                          gctGCACCAACCTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAAcag                                    >  3:40830/3‑118 (MQ=255)
                                                                                                                                          gctGCACCAACCTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCGGTAg     >  4:310270/3‑149 (MQ=255)
                                                                                                                                           ctGCACCAACCTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCGGTAGt    >  3:312154/2‑149 (MQ=255)
                                                                                                                                              cACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCGGTAGTTa  <  6:313937/148‑1 (MQ=255)
                                                                                                                                               aCCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTg                                          <  4:131986/107‑1 (MQ=255)
                                                                                                                                               aCCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTg                                          >  3:131986/1‑107 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
CCCTCGGATTGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCGGTAGTTA  >  NC_002947/1585072‑1585361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑