Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,585,221 T→C 10.0% intergenic (‑208/‑168) PP_1388 ← / → PP_1389 EmrB/QacA family drug resistance transporter/oxaloacetate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,585,2210TC10.0% 129.9 / 5.4 50intergenic (‑208/‑168)PP_1388/PP_1389EmrB/QacA family drug resistance transporter/oxaloacetate decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (25/20);  new base C (3/2);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCGGTAGTTA  >  NC_002947/1585081‑1585361
                                                                                                                                            |                                                                                                                                            
tGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTgca                                                                                                                                       <  7:90235/148‑1 (MQ=255)
tGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATACAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTgcag                                                                                                                                      >  5:165447/1‑149 (MQ=255)
  tAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTgcagc                                                                                                                                     <  4:226305/148‑1 (MQ=255)
  tAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAACCTGGTgcaagc                                                                                                                                    <  4:168905/149‑4 (MQ=255)
       tGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTg                                                                                                                               >  7:133636/1‑149 (MQ=255)
          gTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCg                                                                                                                            >  5:248122/1‑149 (MQ=255)
              ggTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAg                                                                                                                         <  1:318284/148‑1 (MQ=255)
                   cTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGttttt                                                                                                                   <  6:294942/149‑1 (MQ=255)
                   cTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGttttt                                                                                                                   >  4:236956/1‑149 (MQ=255)
                   cTTCCTATGTACTATCTGCATCCCCTAGGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCAGGGGCTTGCACCAGGTGGGTGCAGCAGATTGCGGCAGGgtttt                                                                                                                   >  8:224168/1‑149 (MQ=255)
                               tATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCAT‑GCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGtct                                                                                                      >  7:38655/1‑149 (MQ=255)
                                aTCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGtct                                                                                                      >  5:92216/1‑149 (MQ=255)
                                 tCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGtctc                                                                                                     >  1:319127/1‑149 (MQ=255)
                                    gCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCca                                                                                                  >  2:112401/1‑149 (MQ=255)
                                     cATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGtctc                                                                                                     <  4:101055/145‑1 (MQ=255)
                                          ccTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCtt                                                                                            <  6:92216/149‑1 (MQ=255)
                                           cTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCttt                                                                                           <  2:319127/149‑1 (MQ=255)
                                              tGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTAt                                                                                        >  2:152916/1‑149 (MQ=255)
                                               gCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTAtt                                                                                       >  5:276037/1‑149 (MQ=255)
                                               gCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTAtt                                                                                       >  5:281285/1‑149 (MQ=255)
                                               gCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTAt                                                                                        >  6:186160/1‑148 (MQ=255)
                                                    aTCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCctct                                                                                  >  6:280866/1‑149 (MQ=255)
                                                                       cccAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGa                                                               >  6:197182/2‑149 (MQ=255)
                                                                         cAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGAc                                                              <  6:281285/148‑1 (MQ=255)
                                                                         cAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGAc                                                              <  6:276037/148‑1 (MQ=255)
                                                                               tgcATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGcc                                                          >  4:166095/1‑146 (MQ=255)
                                                                                     gCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGc                                                  >  5:313937/1‑148 (MQ=255)
                                                                                         cGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACgg                                             <  6:248122/149‑1 (MQ=255)
                                                                                            gATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCAAGACCGCCCAGATGGCGACGGTTg                                          >  4:177887/1‑149 (MQ=255)
                                                                                                tCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTCTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGaa                                       <  8:133636/148‑1 (MQ=255)
                                                                                                     ggTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAAcaggt                                  <  3:166095/148‑1 (MQ=255)
                                                                                                       tATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAAcaggtcag                               <  7:224168/149‑1 (MQ=255)
                                                                                                           cctcaGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAAcaggtcagg                              >  8:92468/4‑146 (MQ=255)
                                                                                                             acaGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGAc                         <  5:280866/149‑1 (MQ=255)
                                                                                                                     tGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTa                  >  4:278000/1‑148 (MQ=255)
                                                                                                                     tGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTa                  >  4:134611/1‑148 (MQ=255)
                                                                                                                        aaTCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAgg              <  8:38655/149‑1 (MQ=255)
                                                                                                                            cGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGt           <  1:152916/148‑1 (MQ=255)
                                                                                                                            cGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTc          >  1:326040/1‑149 (MQ=255)
                                                                                                                             ggggCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCCCACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCg         >  4:302369/1‑149 (MQ=255)
                                                                                                                             ggggCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGaa                                       >  6:70726/1‑119 (MQ=255)
                                                                                                                             ggggCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGaa                                       <  5:70726/119‑1 (MQ=255)
                                                                                                                                gCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCGGTa      <  5:186160/149‑1 (MQ=255)
                                                                                                                                 gctGCACCAACCTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAAcag                                    <  4:40830/116‑1 (MQ=255)
                                                                                                                                 gctGCACCAACCTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAAcag                                    >  3:40830/3‑118 (MQ=255)
                                                                                                                                 gctGCACCAACCTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCGGTAg     >  4:310270/3‑149 (MQ=255)
                                                                                                                                  ctGCACCAACCTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCGGTAGt    >  3:312154/2‑149 (MQ=255)
                                                                                                                                     cACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCGGTAGTTa  <  6:313937/148‑1 (MQ=255)
                                                                                                                                      aCCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTg                                          <  4:131986/107‑1 (MQ=255)
                                                                                                                                      aCCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTg                                          >  3:131986/1‑107 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TGTAACCTGAGTTAGGTAGCTTCCTATGTACTATCTGCATCCCCTATGCAAGATCGGCATTGGCGCATTCATCCAGTGCTGCATGGCTGCGTGATATCGTTGGTATGCCACAGAGGCTGAAATCCGGGGCTTGCACCAGGTTGGTGCAGCATATTGCCGCAGGTTTTTCAGTCCGCTGTCTCCCACACCTTTTATTCCTCTGCCTGCATGTCGAGCATGACCGCCCAGATGGCGACGGTTGGAACAGGTCAGGTAGACCCGATTACAGGGGTCGGTAGTTA  >  NC_002947/1585081‑1585361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑