Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,596,417 G→A 8.3% A198A (GCC→GCT)  dctD‑III ← C4‑dicarboxylate transport transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,596,4170GA 8.3% 130.3 / 2.4 48A198A (GCC→GCT) dctD‑IIIC4‑dicarboxylate transport transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (25/19);  new base A (2/2);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

CTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCAGGCCGGCCAGGTCCAGCACTTGCCGGCGCAGCTGTTGCAGGCC  >  NC_002947/1596271‑1596563
                                                                                                                                                   |                                                                                                                                                  
ctctCGTTTTCGTCGAGGTAGAGGGTGCCACCGTTGGCATACTCCAGCTTGCGGTTGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGATCAGTTCCGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAggg                                                                                                                                                   <  1:226795/149‑1 (MQ=255)
ctctCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAggg                                                                                                                                                   <  5:49531/148‑1 (MQ=255)
   tcGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGAGCTa                                                                                                                                                >  3:60281/1‑148 (MQ=255)
   tcGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGAGCTa                                                                                                                                                >  3:55979/1‑148 (MQ=255)
     gATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTa                                                                                                                                                <  4:189129/146‑1 (MQ=255)
     gATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACa                                                                                                                                              >  4:307756/1‑148 (MQ=255)
           gtcgAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCTCACTTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATTGCCGCGCAGTTAAGGGCTACAAAAGGCt                                                                                                                                       >  5:225124/1‑149 (MQ=255)
               aGGAACACGGTGCCACCGTTCGCATACTCCAGCTTGCAGATGCGTTTACCCTGGGCGCCCCTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGCGATGGCCGCGCATATAAGGGCTACAAAGGGCTTgtc                                                                                                                                   >  4:34504/1‑147 (MQ=255)
                 gAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAgg                                                                                                                                                    >  4:173899/1‑130 (MQ=255)
                 gAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAgg                                                                                                                                                    <  3:173899/130‑1 (MQ=255)
                 gAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTggcgg                                                                                                                                 <  3:307756/149‑1 (MQ=255)
                    cacGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGc                                                                                                                                        >  7:118772/1‑139 (MQ=255)
                    cacGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGc                                                                                                                                        <  8:118772/139‑1 (MQ=255)
                       ggTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGACGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACggg                                                                                                                           >  3:36346/1‑149 (MQ=255)
                        gTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGGGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACgggg                                                                                                                          <  3:34504/149‑1 (MQ=255)
                           ccACCGTTGGCATACTCCAGCTCGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGcc                                                                                                                       <  7:98331/149‑1 (MQ=255)
                               cGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCg                                                                                                                      >  6:259720/1‑146 (MQ=255)
                               cGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGaa                                                                                                                    <  5:259720/148‑1 (MQ=255)
                               cGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAg                                                                                                                   <  3:192188/149‑1 (MQ=255)
                                     cATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTg                                                                                                              >  7:294767/1‑148 (MQ=255)
                                       tactacAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAAAAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTgcag                                                                                                           >  6:45000/1‑149 (MQ=255)
                                              gCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCgg                                                                                                    >  4:201126/1‑149 (MQ=255)
                                                ttgactaTGCTTTTACGCTGGGGGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGc                                                                                                  <  6:225124/143‑1 (MQ=255)
                                                    cGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCAcc                                                                                              >  1:107192/1‑149 (MQ=255)
                                                      aTGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCg                                                                                            <  6:83066/149‑1 (MQ=255)
                                                          gTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTcc                                                                                        >  8:68813/1‑149 (MQ=255)
                                                             tACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTg                                                                                     >  7:56826/1‑149 (MQ=255)
                                                              aCCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTg                                                                                     <  5:45000/148‑1 (MQ=255)
                                                               cccTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGc                                                                                    >  5:150474/1‑148 (MQ=255)
                                                                ccTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCa                                                                                  <  6:12353/149‑1 (MQ=255)
                                                                     gcgcCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGcc                                                                             <  6:150474/149‑1 (MQ=255)
                                                                     gcgcCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTCGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGcc                                                                             >  2:5268/1‑149 (MQ=255)
                                                                                ggCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACcgc                                                                  <  3:201126/149‑1 (MQ=255)
                                                                                       ctctCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCaa                                                           >  3:60990/1‑149 (MQ=255)
                                                                                          tcATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAacac                                                        >  1:129676/1‑149 (MQ=255)
                                                                                            aTGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGt                                                      >  4:42267/1‑149 (MQ=255)
                                                                                            aTGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGAGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAacac                                                        <  4:60281/147‑1 (MQ=255)
                                                                                            aTGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGAGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAacac                                                        <  4:55979/147‑1 (MQ=255)
                                                                                                 cGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGGTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCAcc                                                 <  1:5268/149‑1 (MQ=255)
                                                                                                  gAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCg                                                >  2:5942/1‑149 (MQ=255)
                                                                                                             gCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCAggccggc                                      >  6:156631/1‑149 (MQ=255)
                                                                                                                   aaCAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCAGGCCGGCCAGGTc                                >  1:325896/1‑149 (MQ=255)
                                                                                                                      aGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCAGGCCGGCCAGGTCCAg                             <  8:56826/149‑1 (MQ=255)
                                                                                                                      aGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCAGGCCGGCCAGGTCCAg                             <  4:36346/149‑1 (MQ=255)
                                                                                                                        cGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCAGGCCGGCCAGGTCCAGCa                           >  5:263203/1‑149 (MQ=255)
                                                                                                                        cGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCAATCCCGTTTAAACCGCGGATCAACACTTACACCGCCAGGCCGGCCAgtaccacc                            >  4:89114/1‑141 (MQ=255)
                                                                                                                            tCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCAGGCCGGCCAGGTCCAGCACTTg                       >  8:156123/1‑149 (MQ=255)
                                                                                                                                 gATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCAGGCCGGCCAGGTCCAGCACTTGCCGgc                  >  6:178793/1‑149 (MQ=255)
                                                                                                                                       cgcgCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTTCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCAGGCCGGCCAGGTCCAGCACTTGCCGGCGCAGCt            <  3:42267/149‑1 (MQ=255)
                                                                                                                                       cgcgCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCAGGCCGGCCAGGTCCAGCACTTGCCGGCGCAGCt            <  5:156631/149‑1 (MQ=255)
                                                                                                                                             gTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCAGGCCGGCCAGGTCCAGCACTTGCCGGCGCAGCTGTTGCa      <  4:60990/149‑1 (MQ=255)
                                                                                                                                                 aGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGACGGTTTCACCGCGGATCAACACGTTCACCGGCAGGCCGGCCAGGTCCAGCACTTGCCGTCGCAGCTGTTGCAGGcc  >  7:106395/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTCTCGATTTCGTCGAGGAACACGGTGCCACCGTTGGCATACTCCAGCTTGCCGATGCGTTTACCCTGGGCGCCCGTGAAGGCGCCGCTCTCATGGCCGAACAGTT‑CGGCCTCGAACAGCGACTCGGGGATGGCCGCGCAGTTAAGGGCTACAAAGGGCTTGGCGGCACGGGGGCCGAAGTCGTGCAGGCAGCGGGCCACCCGCTCCTTGCCACTGCCGGTTTCACCGCGGATCAACACGTTCACCGGCAGGCCGGCCAGGTCCAGCACTTGCCGGCGCAGCTGTTGCAGGCC  >  NC_002947/1596271‑1596563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑