Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,614,877	A→C	5.4%	L292R (CTG→CGG)	PP_1415 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,614,877	0	A	C	5.4%	231.7 / 3.1	74	L292R (CTG→CGG)	PP_1415	membrane protein
Reads supporting (aligned to +/- strand):  ref base A (41/29);  new base C (2/2);  total (43/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

TCCCACAACCGGAAGAGCGGTTCTGCAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAACGCGCGGTTGAAGTGACAGGC  >  NC_002947/1614736‑1615025                                                                                                                                               |                                                                                                                                                     tCCCACAACCGGAAGAGCGGTTCTGCAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCa                                                                                                                                                <  7:298623/148‑1 (MQ=255) tCCCACAACCGGAAGAGCGGTTCTGCAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAg                                                                                                                                               >  7:255055/1‑149 (MQ=255)   ccACAACCGGAAGAGCGGTTCTGCAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGc                                                                                                                                              <  7:84416/148‑1 (MQ=255)   ccACAACCGGAAGAGCGGTTCTGCAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATGTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGc                                                                                                                                              >  4:17786/1‑148 (MQ=255)    cacaACCGGAAGAGCGGTTCTGCAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCg                                                                                                                                             >  6:260898/1‑148 (MQ=255)      caACCGGAAGAGCGGTTCTGCAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAAc                                                                                                                                          <  2:210573/149‑1 (MQ=255)         ccGGAAGAGCGGTTCTGCAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCGGGC‑TTCATCCCCAGTGTCAGCGAACGc                                                                                                                                        >  5:322226/1‑148 (MQ=255)          cGGAAGAGCGGTTCTGCAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCaa                                                                                                                                      <  4:61016/149‑1 (MQ=255)                agCGGTTCTGCAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAg                                                                                                                                <  6:12678/149‑1 (MQ=255)                agCGGTTCTGCAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAg                                                                                                                                <  3:8730/149‑1 (MQ=255)                    gTTCTGCAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTg                                                                                                                              >  6:242918/1‑147 (MQ=255)                          cAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGtcat                                                                                                                       >  8:242660/1‑148 (MQ=255)                          cAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGtcat                                                                                                                       >  6:12557/1‑148 (MQ=255)                           aaGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGtcatca                                                                                                                     >  4:57110/1‑149 (MQ=255)                                 caacaCCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCCGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCt                                                                                                                 >  2:297513/1‑147 (MQ=255)                                 caacaCCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCCGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCt                                                                                                                 >  2:293261/1‑147 (MQ=255)                                    cacCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTcagca                                                                                                            <  1:323527/149‑1 (MQ=255)                                     acCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCTAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTcag                                                                                                              <  8:81474/146‑1 (MQ=255)                                     acCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCTAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTcag                                                                                                              >  7:81474/1‑146 (MQ=255)                                         ggATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCAc                                                                                                       >  2:303544/1‑149 (MQ=255)                                         ggATCAGGCGCATCACCTGCAGCGCCGGCACCACCCGCACTGACAATTGCAGCGTTTCGGCCGGGACGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCAc                                                                                                       <  6:322226/149‑1 (MQ=255)                                                  gcATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTg                                                                                              >  6:170916/1‑149 (MQ=255)                                                       aCCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCCGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTggcgg                                                                                          <  1:293261/148‑1 (MQ=255)                                                       aCCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCCGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTggcgg                                                                                          <  1:297513/148‑1 (MQ=255)                                                                gcCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCACCAGCCACGCGGCACTGGCGGCAATCGCCAt                                                                                <  1:29588/149‑1 (MQ=255)                                                                  cGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGA‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATg                                                                               >  5:179009/1‑148 (MQ=255)                                                                    tCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGcac                                                                            <  7:242660/149‑1 (MQ=255)                                                                      aCCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACaa                                                                          >  7:209805/1‑149 (MQ=255)                                                                        cAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTggcgg                                                                                          <  2:278280/131‑1 (MQ=255)                                                                        cAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTggcgg                                                                                          >  1:278280/1‑131 (MQ=255)                                                                           cGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCg                                                                     >  6:262359/1‑149 (MQ=255)                                                                             gCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTg                                                                   <  3:57110/149‑1 (MQ=255)                                                                                cTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGc                                                                 >  3:231462/1‑148 (MQ=255)                                                                                   aCAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCAt                                                              >  2:183927/1‑148 (MQ=255)                                                                                     aaTTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATc                                                             >  5:28970/1‑147 (MQ=255)                                                                                     aaTTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATc                                                             >  5:62758/1‑147 (MQ=255)                                                                                       ttGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGCGATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAg                                                          >  1:235375/1‑149 (MQ=255)                                                                                       ttGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAgg                                                         <  5:262359/149‑1 (MQ=255)                                                                                          cAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTgc                                                      >  6:237263/1‑149 (MQ=255)                                                                                          cAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTgc                                                      <  6:179009/149‑1 (MQ=255)                                                                                          cAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTgc                                                      >  3:233830/1‑149 (MQ=255)                                                                                          cAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTgc                                                      >  3:173247/1‑149 (MQ=255)                                                                                           aGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTgcg                                                     >  7:53726/1‑149 (MQ=255)                                                                                            gCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTgcgc                                                    <  5:170916/149‑1 (MQ=255)                                                                                             cGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGt                                                        <  7:185263/144‑1 (MQ=255)                                                                                             cGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGt                                                        >  8:185263/1‑144 (MQ=255)                                                                                               tttCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGcccc                                                 <  5:260898/149‑1 (MQ=255)                                                                                                 tCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAg                                               >  6:252469/1‑149 (MQ=255)                                                                                                  cGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAg                                               >  4:3115/1‑148 (MQ=255)                                                                                                            gCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTg                                    >  6:270223/1‑149 (MQ=255)                                                                                                            gCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTg                                    >  8:186589/1‑149 (MQ=255)                                                                                                            gCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATAGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTg                                    >  8:181795/1‑149 (MQ=255)                                                                                                              tCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCATTCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTgcg                                  >  2:172897/1‑149 (MQ=255)                                                                                                              tCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAAc                                                                         >  2:173946/1‑110 (MQ=255)                                                                                                              tCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAAc                                                                         <  1:173946/110‑1 (MQ=255)                                                                                                                 tttCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCgcgg                               <  3:3115/149‑1 (MQ=255)                                                                                                                     ggCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGga                           <  1:129240/149‑1 (MQ=255)                                                                                                                      gCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGcggcgg                            >  2:2578/1‑147 (MQ=255)                                                                                                                      gCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGgaa                          <  8:209805/149‑1 (MQ=255)                                                                                                                          tCCCGCCAGGC‑ATCATCCCCAGTGTCAGCTAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGTGCGGCGgaagaa                       <  8:255055/148‑1 (MQ=255)                                                                                                                          tCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCGCCAGGAACGCCGGTGCGCGGCGGAAGAAc                      >  2:97629/1‑149 (MQ=255)                                                                                                                            ccGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGCCGGTGCGCGGCGGAAGAAcgc                    >  7:93863/1‑149 (MQ=255)                                                                                                                              gCCAGGG‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAAcgcgc                  <  6:62758/149‑1 (MQ=255)                                                                                                                               ccAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAAcgcgcg                 <  4:233830/149‑1 (MQ=255)                                                                                                                               ccAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAAcgcgcg                 >  5:99756/1‑149 (MQ=255)                                                                                                                                   gC‑ATCATCCCCAGTGTCAGCGAACGGAAGTGGAGGGGAGTCAGCATGGTCAGCCGCCACGCGGCACTGGCGGCAATCGCCGTGCGCAGCGCCGTGGCCATCATGGTGCGCCCCAGGACCGACGGTGCGGGGCGGAAGAGCGCGCGGTTg             >  2:276714/1‑149 (MQ=255)                                                                                                                                   gC‑ATCATCCCCAGTGACAGCGAACGCAAGTCCAGTGCAGTCATCATGCTAAGCCGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAACGCGCGGtt              >  2:239169/1‑148 (MQ=255)                                                                                                                                    c‑atcatCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAACGCGCGGTTGa            <  5:242918/149‑1 (MQ=255)                                                                                                                                       tcctcCCCAGTGGCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAACGCGCGGTTGAAg          <  3:245098/146‑1 (MQ=255)                                                                                                                                       tcatcCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAACGCGCGGTTGAAg          >  3:151424/1‑149 (MQ=255)                                                                                                                                            cccAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAACGCGCGGTTGAAGTGa       >  3:224708/1‑147 (MQ=255)                                                                                                                                             ccAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAACGCGCGGTTGAAg          >  2:12902/1‑143 (MQ=255)                                                                                                                                             ccAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAACGCGCGGTTGAAg          <  1:12902/143‑1 (MQ=255)                                                                                                                                             ccAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAACGCGCGGTTGAAGTGAcag    <  2:235375/149‑1 (MQ=255)                                                                                                                                               aGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAACGCGCGGTTGAAGTGAcaggc  <  5:12557/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                     TCCCACAACCGGAAGAGCGGTTCTGCAAGAAACAACACCAGGATCAGGCGCATCACCTGCAGCGCCGTCACCAGCGGCACTGACAATTGCAGCGTTTCGGCCGTGAGGCTCATTTCGGCGATCCCGCCAGGC‑ATCATCCCCAGTGTCAGCGAACGCAAGTCCAGTGCAGTCATCATGCTCAGCAGCCACGCGGCACTGGCGGCAATCGCCATGCACAACGCCGTGGCCATCAAGGTGCGCCCCAGGAACGACGGTGCGCGGCGGAAGAACGCGCGGTTGAAGTGACAGGC  >  NC_002947/1614736‑1615025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑