Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,629,453 G→T 7.3% Q321H (CAG→CAT)  mucB → sigma factor AlgU regulator MucB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,629,4530GT 7.3% 214.7 / 4.2 68Q321H (CAG→CAT) mucBsigma factor AlgU regulator MucB
Reads supporting (aligned to +/- strand):  ref base G (32/31);  new base T (3/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

ACCCAGCTAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAGGGGTCTT  >  NC_002947/1629307‑1629600
                                                                                                                                                  |                                                                                                                                                   
aCCCAGCTAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGt                                                                                                                                                    >  7:25724/1‑148 (MQ=255)
    aGCTAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATgg                                                                                                                                               >  7:259519/1‑149 (MQ=255)
       tAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCATTACTGgcgc                                                                                                                                            <  6:195995/149‑1 (MQ=255)
           cccGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCtt                                                                                                                                        <  8:212335/149‑1 (MQ=255)
            ccGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCttt                                                                                                                                       >  6:2434/1‑149 (MQ=255)
               aCCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATTCCCAGGCGCGCCAGTAATGGCGCGCTTTTGc                                                                                                                                    <  4:225850/149‑1 (MQ=255)
               aCCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGAAGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGc                                                                                                                                    >  5:169680/1‑149 (MQ=255)
                  tCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTg                                                                                                                                 >  1:13770/1‑149 (MQ=255)
                  tCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACCCGACGCCCTGTCGATGCGCACCCCGGAGGCCCAGCCGCGCCAGTAATGGCGCGCTTTTGCTTg                                                                                                                                 >  2:209776/1‑149 (MQ=255)
                    ggCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAAGCGCGCCATTACTGgcgcgcctgg                                                                                                                                      >  6:3758/1‑138 (MQ=255)
                    ggCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAAGCGCGCCATTACTGgcgcgcctgg                                                                                                                                      <  5:3758/142‑5 (MQ=255)
                     gCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGcc                                                                                                                               >  3:50130/1‑148 (MQ=255)
                       aGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAg                                                                                                                            <  4:159089/149‑1 (MQ=255)
                             gcgTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAg                                                                                                                       <  1:247389/148‑1 (MQ=255)
                                   ccTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTg                                                                                                                <  4:50130/149‑1 (MQ=255)
                                   ccTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTg                                                                                                                <  7:29601/149‑1 (MQ=255)
                                    cTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTgg                                                                                                               >  7:247240/1‑149 (MQ=255)
                                    cTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTgg                                                                                                               <  5:55960/149‑1 (MQ=255)
                                       aaTACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGc                                                                                                             <  3:279891/148‑1 (MQ=255)
                                        aTACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCa                                                                                                            >  7:78991/1‑148 (MQ=255)
                                            acCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGc                                                                                                       >  1:271601/1‑149 (MQ=255)
                                              ccAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCt                                                                                                     >  3:13082/1‑149 (MQ=255)
                                              ccAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCt                                                                                                     >  8:21747/1‑149 (MQ=255)
                                               cAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCt                                                                                                     >  2:300703/1‑148 (MQ=255)
                                                   ggCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCtgaaat                                                                                                >  2:297209/1‑149 (MQ=255)
                                                    gCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTACTGGCGCGCTTTTGCGTGCCGAGCCAGGGCTGGTGGGCAGACGCCtgaaat                                                                                                >  8:255341/1‑148 (MQ=255)
                                                    gCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGc                                                                                                             >  8:226448/1‑135 (MQ=255)
                                                          tGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTa                                                                                         <  7:64939/149‑1 (MQ=255)
                                                            aTGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTaaa                                                                                       <  8:247240/149‑1 (MQ=255)
                                                            aTGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTaaa                                                                                       <  8:25724/149‑1 (MQ=255)
                                                            aTGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTaa                                                                                        <  7:21747/148‑1 (MQ=255)
                                                             tGGGGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGTCGCGCTTTTGCGTGTCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAg                                                                                      <  1:209776/149‑1 (MQ=255)
                                                              ggTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAg                                                                                      >  5:305785/1‑148 (MQ=255)
                                                              ggTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGACCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAg                                                                                      >  5:286713/1‑148 (MQ=255)
                                                                 gACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCAtt                                                                                  >  2:115944/1‑149 (MQ=255)
                                                                   ccGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTg                                                                                 >  8:271597/1‑148 (MQ=255)
                                                                    cGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGt                                                                                >  3:303123/1‑148 (MQ=255)
                                                                        gTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCAttt                                                                           <  5:137153/149‑1 (MQ=255)
                                                                            gCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCaa                                                                       <  2:288740/149‑1 (MQ=255)
                                                                            gCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCaa                                                                       <  5:2434/149‑1 (MQ=255)
                                                                            gCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCaa                                                                       <  4:303123/149‑1 (MQ=255)
                                                                              gAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATc                                                                     >  6:153304/1‑149 (MQ=255)
                                                                                     cGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTg                                                                          >  5:30333/1‑137 (MQ=255)
                                                                                     cGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTg                                                                          <  6:30333/137‑1 (MQ=255)
                                                                                            tACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAAtttt                                                       <  2:271601/149‑1 (MQ=255)
                                                                                            tACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATGGTCATTTGCAATCCATCGGCAAAtttt                                                       >  6:96543/1‑149 (MQ=255)
                                                                                             aCGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTc                                                      >  8:99070/1‑149 (MQ=255)
                                                                                              cGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTc                                                      <  6:305785/148‑1 (MQ=255)
                                                                                                  aGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAg                                                 <  7:99070/149‑1 (MQ=255)
                                                                                                      cgcgTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCa                                             <  5:153304/149‑1 (MQ=255)
                                                                                                             cccTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTg                                      <  8:78991/149‑1 (MQ=255)
                                                                                                              ccTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTg                                      >  8:172604/1‑148 (MQ=255)
                                                                                                               cTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGaa                                    <  4:13082/149‑1 (MQ=255)
                                                                                                                  tCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAaga                                  <  6:169680/148‑1 (MQ=255)
                                                                                                                  tCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAag                                   <  1:286437/147‑1 (MQ=255)
                                                                                                                      tGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTg                             <  1:300703/149‑1 (MQ=255)
                                                                                                                            cccAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCttt                       >  6:116926/1‑149 (MQ=255)
                                                                                                                            cccAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCttt                       >  6:116924/1‑149 (MQ=255)
                                                                                                                                      ccAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTtgt             >  4:87462/1‑149 (MQ=255)
                                                                                                                                       cAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTATCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTtgtg            >  8:76097/1‑149 (MQ=255)
                                                                                                                                        aagcgcgcCATTACTGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTtgtg            >  7:226008/3‑148 (MQ=255)
                                                                                                                                        aagcgcgcCATTACTGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTtgtg            >  7:154547/3‑148 (MQ=255)
                                                                                                                                            gcgcCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAggg       <  6:286713/149‑1 (MQ=255)
                                                                                                                                            gcgcCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAggg       <  7:255341/149‑1 (MQ=255)
                                                                                                                                            gcgcCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAggg       <  1:115944/149‑1 (MQ=255)
                                                                                                                                                cAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGa                                     >  4:120181/1‑115 (MQ=255)
                                                                                                                                                cAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGa                                     <  3:120181/115‑1 (MQ=255)
                                                                                                                                                 aGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAGGGGTCtt  >  6:13363/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ACCCAGCTAGGCCCGACCTCGGCAGTCTCGCGTCGCCTGAATACACCCAAGGGCAAAGTGATGGTGACCGTGGTTGGCGAAATTCCGCTGGGTACGGCAGAACGCGTCGCCCTGTCGATGCGCCCCCAGGATGCCCAGGCGCGCCAGTAATGGCGCGCTTTTGCGTGCCGAGCCAAGGCAGGTGGGCATACGCCTGAAATGAAATTAAAGCATTGTCATTTGCAATCCATCGGCAAATTTTCTATAGGTCAGGCTTTGAAGAGCCTGGCCTTTTCTGCGTTGTGCAGGGGTCTT  >  NC_002947/1629307‑1629600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑