Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,645,887 G→C 9.2% S490R (AGC→AGG)  gcd ← quinoprotein glucose dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,645,8870GC 9.2% 151.9 / 2.3 50S490R (AGC→AGG) gcdquinoprotein glucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (21/24);  new base C (3/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTTGTGGGGTGGGGATCTCGCGGATCGGCACGATTGGCGTACCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTGCGGTCGGCGCCCCACTGGTCCGGG  >  NC_002947/1645743‑1646028
                                                                                                                                                |                                                                                                                                              
ggcTTGTGGGGTGGGGATCTCGCGGATCGGCACGATTGGCGTACCGTCGCGGCTGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGc                                                                                                                                            <  8:22640/147‑1 (MQ=255)
        gggtggggATCTCGCGGATCGGCACGATTGGCGTACCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTcc                                                                                                                                    <  1:308020/149‑1 (MQ=255)
        gggtggggATCTCGCGGATCGGCACGATTGGCGTACCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTcc                                                                                                                                    <  2:202737/149‑1 (MQ=255)
        gggtggggATCTCGCGGATCGGCACGATTGGCGTACCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCAGCCAGCCGAccct                                                                                                                                     <  8:70468/148‑4 (MQ=255)
             gggATCTCGCGGATCGGCACGATTGGCGTACCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTc                                                                                                                               >  1:2053/1‑149 (MQ=255)
              ggATCTCGCGGATCGGCACGATTGGCGTACCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTcc                                                                                                                              <  4:103991/149‑1 (MQ=255)
                            ggCACGATTGGCGTACCGTTGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCgtggt                                                                                                                 <  2:84271/148‑1 (MQ=255)
                             gCACGATTGGCGTACCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGtggtgg                                                                                                               <  2:44049/149‑1 (MQ=255)
                             gCACGATTGGCGTACCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTAGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGtggtgg                                                                                                               >  6:322067/1‑149 (MQ=255)
                                   ttGGCGTACCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAAc                                                                                                         <  1:317957/149‑1 (MQ=255)
                                   ttGGCGTACCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAAc                                                                                                         <  2:33651/149‑1 (MQ=255)
                                       cGTACCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGAt                                                                                                     >  3:259653/1‑149 (MQ=255)
                                          aCCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGt                                                                                                  >  5:116412/1‑149 (MQ=255)
                                          aCCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGt                                                                                                  >  8:2577/1‑149 (MQ=255)
                                                    cggTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGc                                                                                        >  6:58612/1‑149 (MQ=255)
                                                      gTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATg                                                                                                                                 >  1:77726/1‑106 (MQ=255)
                                                      gTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATg                                                                                                                                 <  2:77726/106‑1 (MQ=255)
                                                      gTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCt                                                                                      <  7:57413/149‑1 (MQ=255)
                                                         gAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGc                                                                                   >  2:256549/1‑149 (MQ=255)
                                                             aCGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑Gt                                                                              >  2:271832/1‑149 (MQ=255)
                                                              cGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTg                                                                             <  7:135715/149‑1 (MQ=255)
                                                              cGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTg                                                                             <  2:311893/149‑1 (MQ=255)
                                                              cGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTg                                                                             <  3:253815/149‑1 (MQ=255)
                                                                tACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGc                                                                           <  8:144383/149‑1 (MQ=255)
                                                                 aCAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGc                                                                           >  7:228624/1‑148 (MQ=255)
                                                                    ggCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAgg                                                                       >  4:5030/1‑149 (MQ=255)
                                                                         ccTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAg                                                                  >  7:130874/1‑149 (MQ=255)
                                                                             gcttgGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGc                                                               >  6:291077/1‑148 (MQ=255)
                                                                                      gTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCg                                                     <  2:120059/149‑1 (MQ=255)
                                                                                         cGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGgcg                                                  <  7:2577/149‑1 (MQ=255)
                                                                                                          acacCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGcc                                 >  4:290092/1‑149 (MQ=255)
                                                                                                            acCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCg                               >  8:107234/1‑149 (MQ=255)
                                                                                                              cATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCggg                             >  4:75794/1‑149 (MQ=255)
                                                                                                               aTCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCgggg                            >  7:56438/1‑149 (MQ=255)
                                                                                                                      gTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTGCGGTc                     <  2:174988/149‑1 (MQ=255)
                                                                                                                      gTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTGCGGTc                     <  5:58612/149‑1 (MQ=255)
                                                                                                                          tCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTg                          >  1:25641/1‑140 (MQ=255)
                                                                                                                          tCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTg                          <  2:25641/140‑1 (MQ=255)
                                                                                                                                 tACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTGCGGTCGGCGCCCCACt          >  7:294454/1‑149 (MQ=255)
                                                                                                                                   tggacgTCGGCAGCCAGCCGACCTCCATGTCCCACAGCTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCAAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTGCGGTCGGCGCCCCACTgg        >  3:150526/6‑149 (MQ=255)
                                                                                                                                    cAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTGCGGTCGGCGccc              <  5:240773/142‑1 (MQ=255)
                                                                                                                                    cAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTGCGGTCGGCGccc              >  6:240773/1‑142 (MQ=255)
                                                                                                                                    cAGGGTCCGCTGCCTGCCGACGTCCATGTCCCACAGGTCGAGGTGGGTGAACTGATAGTTCAAGCGTGCCTTGAAC‑GTGGCCAGGTCAAGGTCCAACACCCCGGCGCTGTACTTCTCGGCGACCGGGGTGCGGTCGGCGACCCACTgct       >  2:186134/1‑147 (MQ=255)
                                                                                                                                      acgTCGGCAGCCAGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTGCGGTCGGCGCCCCACTGGTcc     >  1:213591/3‑149 (MQ=255)
                                                                                                                                       cgTCGGCAGCCAGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTGCGGTCGGCGCCCCACTGGTCCg    <  7:100642/148‑1 (MQ=255)
                                                                                                                                         tCGGCTGGCTGCCGACGTCCATGTGCGACTGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTGCGGTCGGCGCCCCACTGGTCCggg  <  3:305121/149‑1 (MQ=255)
                                                                                                                                         tCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTAc                                            >  3:286169/1‑107 (MQ=255)
                                                                                                                                         tCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTAc                                            <  4:286169/107‑1 (MQ=255)
                                                                                                                                         tCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTGCGGTCGGCGCCCCACTGGTCCggg  <  3:75794/149‑1 (MQ=255)
                                                                                                                                         tCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTGCGGTCGGCGCCCCACTGGTCCggg  <  4:54019/149‑1 (MQ=255)
                                                                                                                                                gctgCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCGAGTGGCCAGGTCAAGGGCGACCACGCCGGCGCTg                                               <  1:38962/98‑1 (MQ=255)
                                                                                                                                                gctgCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTg                                               >  2:38962/1‑97 (MQ=255)
                                                                                                                                                |                                                                                                                                              
GCCTTGTGGGGTGGGGATCTCGCGGATCGGCACGATTGGCGTACCGTCGCGGCGGTCGAGCACGTACAGGCTGCCTTGCTTGGTCGGTACGATGATCGCCGGTTTCACACCATCGTCGGTTTTCAGGTGTACCAGGGTCGGCTGGCTGCCGACGTCCATGTCCCACAGGTCGTGGTGGGTGAACTGATAGTTCCAGCGTGCCTTGCCG‑GTGGCCAGGTCAAGGGCGACCACGCCGGCGCTGTACTTCTCGGCGCCCGGGGTGCGGTCGGCGCCCCACTGGTCCGGG  >  NC_002947/1645743‑1646028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑