Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,748,944	A→T	9.4%	intergenic (‑277/‑55)	PP_1548 ← / → PP_5744	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,748,944	0	A	T	9.4%	174.6 / 8.5	64	intergenic (‑277/‑55)	PP_1548/PP_5744	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (25/33);  new base T (2/4);  total (27/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

CAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAGGGTTTCAGCGATGATCAACTCGCCGCTGG  >  NC_002947/1748801‑1749092                                                                                                                                                  |                                                                                                                                                     caGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGTGCTTGCATTTA‑AAAAACAA‑GCATGCTTTTATAAATGCAAGTTCGCtt                                                                                                                                                 <  5:221018/149‑1 (MQ=25)   gAAGTCAATCGCCGGATTAACTCGTCGCCTGGTGGCTCATCTGCTGTTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCTAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTtgt                                                                                                                                               <  3:299594/149‑1 (MQ=255)     aCTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGATCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGTGCTTGCATTTA‑AAAAACAA‑GCATGCTTTTATAAATGCAAGTTCGCTtgcat                                                                                                                                             <  6:167323/149‑4 (MQ=21)       tCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTaa                                                                                                                                           >  8:280292/1‑149 (MQ=255)              ccGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTtctc                                                                                                                                    <  8:159888/149‑1 (MQ=255)              ccGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTtctc                                                                                                                                    >  6:297406/1‑149 (MQ=255)                 gATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTa                                                                                                                                 <  1:233335/149‑1 (MQ=255)                   ttAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACt                                                                                                                               >  7:116113/1‑149 (MQ=255)                      aCTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGTGCTTGCATTTA‑AAAAACAA‑GCATGCTTTTATAAATGCAAGTTCGCTtgca                                                                                                                                              <  6:133968/131‑3 (MQ=18)                      aCTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGTGCTTGCATTTA‑AAAAACAA‑GCATGCTTTTATAAATGCAAGTTCGCTtgca                                                                                                                                              >  5:133968/1‑129 (MQ=18)                      aCTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCTCGCTTGTGTAATTTTCTCTTACTGTa                                                                                                                            >  6:142336/1‑149 (MQ=255)                        tCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATg                                                                                                                          >  1:161262/1‑149 (MQ=255)                        tCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATg                                                                                                                          >  3:271337/1‑149 (MQ=255)                          ggCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTTCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATCGCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTAt                                                                                                                           <  1:240294/147‑1 (MQ=255)                                      cTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGTCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAgtgtgt                                                                                                            <  6:272733/149‑1 (MQ=255)                                         aTCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTa                                                                                                           <  7:280292/147‑1 (MQ=255)                                             gctgGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGTAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCt                                                                                                     >  8:132780/1‑149 (MQ=255)                                              ctgGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGa                                                                                                        <  2:198190/145‑1 (MQ=255)                                              ctgGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGa                                                                                                        <  4:271337/145‑1 (MQ=255)                                               tgGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCttt                                                                                                   >  2:313334/1‑149 (MQ=255)                                                 gTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCg                                                                                                 >  8:140428/1‑149 (MQ=255)                                                 gTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCg                                                                                                 <  5:109525/149‑1 (MQ=255)                                                    tttAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGa                                                                                               <  7:112074/148‑1 (MQ=255)                                                                tGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAgg                                                                                   >  2:66202/1‑148 (MQ=255)                                                                     gCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAagag                                                                             <  6:230536/149‑1 (MQ=255)                                                                               ccAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTa                                                                    <  3:209397/148‑1 (MQ=255)                                                                               ccAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTAt                                                                   >  4:288188/1‑149 (MQ=255)                                                                                 aGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATg                                                                  >  5:247392/1‑148 (MQ=255)                                                                                   cTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGaa                                                               <  7:140428/149‑1 (MQ=255)                                                                                      gATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAagag                                                            >  7:222541/1‑149 (MQ=255)                                                                                      gATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAaga                                                             <  3:288188/148‑1 (MQ=255)                                                                                            aatgcaaGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAAc                                                       >  5:280518/1‑148 (MQ=255)                                                                                              tgcaaGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAg                                                    >  6:81033/1‑149 (MQ=255)                                                                                                    cGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCTCGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGa                                              <  5:142336/149‑1 (MQ=255)                                                                                                     gAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAg                                             <  1:66202/149‑1 (MQ=255)                                                                                                     gAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAg                                             <  1:297202/149‑1 (MQ=255)                                                                                                      aaCTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGc                                            <  2:258308/149‑1 (MQ=255)                                                                                                         ttGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTAt                                                                   >  3:246567/1‑123 (MQ=255)                                                                                                         ttGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTAt                                                                   <  4:246567/123‑1 (MQ=255)                                                                                                         ttGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGc                                         >  6:242800/1‑149 (MQ=255)                                                                                                           gCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTg                                       >  6:236964/1‑149 (MQ=255)                                                                                                            cATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTgg                                      <  5:297406/149‑1 (MQ=255)                                                                                                             aTTTATAATAGCTT‑GCTTG‑TTTTTTAAATGCAAGCACGGTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGATAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTgg                                      <  5:28557/148‑1 (MQ=255)                                                                                                             aTTTATAAAAGCAT‑GCTTG‑ATTTTTAAATGCAAGCACGCTTGTGTAATTTTCACTTACTGTAAGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGCCGCAGGAAAGAGCAGTTAGTATGGAAGAGCTAACGAGCCTTGAGCCGCTgg                                      >  6:28557/1‑148 (MQ=255)                                                                                                              tttATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGc                                    >  6:282359/1‑149 (MQ=255)                                                                                                              tttATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGc                                    <  7:132780/149‑1 (MQ=255)                                                                                                               ttATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCt                                   >  3:62091/1‑149 (MQ=255)                                                                                                                tataAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCtt                                  >  5:97459/1‑149 (MQ=255)                                                                                                                  taAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGa                                <  2:161262/149‑1 (MQ=255)                                                                                                                    aaaGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAgg                              <  1:313334/149‑1 (MQ=255)                                                                                                                     aaGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAggg                             >  1:158770/1‑149 (MQ=255)                                                                                                                     aaGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCT‑ACGAGCCTTGAGCGGCTGGGCTTGAgg                              <  4:9933/147‑1 (MQ=255)                                                                                                                     aaGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCT‑ACGAGCCTTGAGCGGCTGGGCTTGAgg                              >  3:9933/1‑147 (MQ=255)                                                                                                                                g‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGAAGTTAGTATGGAAGAGCTAACGAGACTTGAGCTGATGGGCTTGATGGTTTCAGCgat                   >  8:93804/1‑149 (MQ=255)                                                                                                                                    ttttAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAGGGTTTCAGCgatga                 <  6:280518/148‑1 (MQ=255)                                                                                                                                     tttAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAGGGTTTCAGCgatgat                <  7:233602/148‑1 (MQ=255)                                                                                                                                      ttAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAGGGTTTCAGCGATGATc               <  5:282359/148‑1 (MQ=255)                                                                                                                                        aaaTGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCATTGTGTAGGAGCTTTCGGATGTCGGCGCTCGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAGGGTTTCAGCGATGATCaa             <  7:93804/148‑1 (MQ=255)                                                                                                                                        aaaTGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAGGGTTTCAGCGATGATCaa             <  5:81033/148‑1 (MQ=255)                                                                                                                                             cAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAgg                              >  2:35355/1‑126 (MQ=255)                                                                                                                                             cAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAgg                              <  1:35355/126‑1 (MQ=255)                                                                                                                                             cAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAGGGTTTCAGCGATGATCAACTcgc        <  8:26150/148‑1 (MQ=255)                                                                                                                                             cAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAGGGTTTCAGCGATGATCAACTcgc        <  8:31019/148‑1 (MQ=255)                                                                                                                                                 cACGCTTGTGTAATTTTCTCTTACTGTATGGAGGTCCAGTGTGTAGGAGCTTTCGGATGTCTGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAGGGTTTCAGCGATGATCAACTCGCCGCTg   <  8:116113/149‑1 (MQ=255)                                                                                                                                                  aCGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAGGGTTTCAGCGATGATCAACTCGCCGCTgg  <  6:247392/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                     CAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCAT‑GCTTG‑TTTTTTAAATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGAGCGGCTGGGCTTGAGGGTTTCAGCGATGATCAACTCGCCGCTGG  >  NC_002947/1748801‑1749092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑