Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,808,075 G→A 7.8% V42V (GTG→GTA)  kdsA‑I → 2‑dehydro‑3‑deoxyphosphooctonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,808,0750GA 7.8% 187.3 / 4.0 64V42V (GTG→GTA) kdsA‑I2‑dehydro‑3‑deoxyphosphooctonate aldolase
Reads supporting (aligned to +/- strand):  ref base G (30/29);  new base A (2/3);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

AGAAGAACAAGGCCTGATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGT  >  NC_002947/1807930‑1808218
                                                                                                                                                 |                                                                                                                                               
agaataACAAGGCCTGATCGATTACCCATAAGATCATTCGCGTCGTTAAAATCGAGATCGCCAACGACAAGCCTTTCTTCCTGTTCGTCGGCATGAACGTTCTGTAATCCCGTGACCTGGCGATTAAGGTCTGCGAAGAGTACGTGCgg                                                                                                                                              <  7:202318/149‑1 (MQ=255)
        aaGGCCTGATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCga                                                                                                                                      <  7:253263/149‑1 (MQ=255)
               gATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTc                                                                                                                               >  7:193402/1‑149 (MQ=255)
                aTCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCg                                                                                                                              <  8:193402/149‑1 (MQ=255)
                     tGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGCTTCTCGGTaac                                                                                                                         <  1:162080/149‑3 (MQ=255)
                     tGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTaac                                                                                                                         <  3:255079/149‑3 (MQ=255)
                         ccAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGCTTCTCGGTaac                                                                                                                         <  1:133498/145‑3 (MQ=255)
                         ccAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGCTTCTCGGTaac                                                                                                                         >  2:133498/1‑143 (MQ=255)
                         ccAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCg                                                                                                                      >  3:244059/1‑148 (MQ=255)
                          cAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTa                                                                                                                    <  3:16142/149‑1 (MQ=255)
                          cAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTa                                                                                                                    <  4:210800/149‑1 (MQ=255)
                           agaagaTGATTCTCTTCTGTAACATCGAGATCGCCAACTACAAGCCGTTCGTCCTGTTCGGCTGCATGAACGTTCTGGAATCCCGTGACATGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAATCTCGGTATCCCGTAc                                                                                                                   <  6:146440/149‑1 (MQ=255)
                           agaagaTCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTAc                                                                                                                   >  6:97494/1‑149 (MQ=255)
                                aTCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACgtgt                                                                                                               >  3:61861/1‑148 (MQ=255)
                                 tCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTc                                                                                                             <  4:102321/149‑1 (MQ=255)
                                   aTTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTc                                                                                                             >  1:151801/1‑147 (MQ=255)
                                       gcgTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAAcc                                                                                                                        <  6:76166/132‑1 (MQ=255)
                                       gcgTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAAcc                                                                                                                        >  5:76166/1‑132 (MQ=255)
                                             gTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTc                                                                                                 <  5:97494/149‑1 (MQ=255)
                                             gTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTc                                                                                                 >  7:236863/1‑149 (MQ=255)
                                             gTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTc                                                                                                 >  1:219417/1‑149 (MQ=255)
                                                  aTCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTAc                                                                                                                   >  3:154579/1‑126 (MQ=255)
                                                  aTCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTAc                                                                                                                   <  4:154579/126‑1 (MQ=255)
                                                  aTCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGAc                                                                                              >  4:198191/1‑147 (MQ=255)
                                                      agaTCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGc                                                                                         >  6:241211/1‑148 (MQ=255)
                                                             cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                 <  2:272878/149‑1 (MQ=255)
                                                             cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                 <  4:75301/149‑1 (MQ=255)
                                                             cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                 >  6:316931/1‑149 (MQ=255)
                                                             cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                 >  1:144639/1‑149 (MQ=255)
                                                             cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGAGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                 >  5:242522/1‑149 (MQ=255)
                                                              aaCGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTtc                                                                                <  3:198191/149‑1 (MQ=255)
                                                              aaCGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTtc                                                                                <  7:175694/149‑1 (MQ=255)
                                                                 gACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTtcgtc                                                                             <  8:236863/149‑1 (MQ=255)
                                                                   cAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATGCGGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCACGTAAGTGTTCAAGGACAGTGTCGACAAGGCCAACAGTTCGTCgg                                                                           >  7:139001/1‑149 (MQ=255)
                                                                     aGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAgg                                                                                                         >  8:253826/1‑117 (MQ=255)
                                                                     aGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAgg                                                                                                         <  7:253826/117‑1 (MQ=255)
                                                                      gCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTaa                                                                        >  4:273114/1‑149 (MQ=255)
                                                                      gCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAACCCgc                                                                                                                     <  5:91588/104‑2 (MQ=37)
                                                                      gCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAACCCgc                                                                                                                     >  6:91588/1‑103 (MQ=37)
                                                                              tCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTAc                                                                >  2:152458/1‑149 (MQ=255)
                                                                              tCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTAc                                                                <  2:158394/149‑1 (MQ=255)
                                                                               ccTGTTCGGAGGCATGAACGTTCTGGAATCGCGTGACCTGGCGATGAAGGTATGCGAAGAGTACGTGAGGGTTACAGAGAAGCTCGGTATCCCGTACTTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAAAGTCCTaaa                                                               >  7:130024/1‑147 (MQ=255)
                                                                                   ttCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTgg                                                           <  1:152458/149‑1 (MQ=255)
                                                                                         ggcATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTTCGAAGAGTACGTGCGGTTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAATGCCAGTTTCGACAAGGCCAACCGTTAGTCGGTAACGTCCTACCGTGGCCCTgg                                                     <  8:139001/149‑1 (MQ=255)
                                                                                          gcATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATACCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTgg                                                     >  7:85134/1‑148 (MQ=255)
                                                                                                     cTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAgg                                          <  2:219417/148‑1 (MQ=255)
                                                                                                      tGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAgggg                                        >  7:69389/1‑149 (MQ=255)
                                                                                                       ggAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGc                                       >  6:37159/1‑149 (MQ=255)
                                                                                                          aTCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTg                                     >  2:148209/1‑148 (MQ=255)
                                                                                                             ccGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTg                                     >  1:207222/1‑145 (MQ=255)
                                                                                                             ccGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTg                                     >  4:306608/1‑145 (MQ=255)
                                                                                                                  aCCTGGCTAAGAAGGTCTGCGAAGAGTAAGTGCGGGTTACCTAGAAGCTCTGTATACCGTACGTTTTCAATGCCATTTTCGACAATGACAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCtt                             <  8:130024/148‑1 (MQ=255)
                                                                                                                  aCCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCtt                             <  5:241211/148‑1 (MQ=255)
                                                                                                                  aCCTGGCGATGAAGGTCTGCGAAGAGTACGAGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCtt                             <  6:242522/148‑1 (MQ=255)
                                                                                                                       gCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGaa                         <  7:171102/147‑1 (MQ=255)
                                                                                                                       gCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGaa                         <  2:207222/147‑1 (MQ=255)
                                                                                                                          aTGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCa                                                                                                      >  7:103806/1‑67 (MQ=255)
                                                                                                                          aTGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCa                                                                                                      <  8:103806/67‑1 (MQ=255)
                                                                                                                          aTGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGaa                         >  1:293905/1‑144 (MQ=255)
                                                                                                                                gTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAgcg              >  2:301062/1‑149 (MQ=255)
                                                                                                                                     cGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCt         >  6:194550/1‑149 (MQ=255)
                                                                                                                                      gAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCtt        >  8:272358/1‑149 (MQ=255)
                                                                                                                                          agTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAAc    <  5:316931/149‑1 (MQ=255)
                                                                                                                                             aCGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGt  <  2:151801/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AGAAGAACAAGGCCTGATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGT  >  NC_002947/1807930‑1808218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑