Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,808,090 G→T 14.2% K47N (AAG→AAT) ‡ kdsA‑I → 2‑dehydro‑3‑deoxyphosphooctonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,808,0900GT14.2% 178.0 / 17.8 69K47N (AAG→AAT) ‡kdsA‑I2‑dehydro‑3‑deoxyphosphooctonate aldolase
Reads supporting (aligned to +/- strand):  ref base G (30/29);  new base T (4/6);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

GATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGATCACCGATGTGC  >  NC_002947/1807945‑1808238
                                                                                                                                                 |                                                                                                                                                    
gATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTc                                                                                                                                                   >  7:193402/1‑149 (MQ=255)
 aTCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCg                                                                                                                                                  <  8:193402/149‑1 (MQ=255)
      tGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGCTTCTCGGTaac                                                                                                                                             <  1:162080/149‑3 (MQ=255)
      tGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTaac                                                                                                                                             <  3:255079/149‑3 (MQ=255)
          ccAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGCTTCTCGGTaac                                                                                                                                             <  1:133498/145‑3 (MQ=255)
          ccAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGCTTCTCGGTaac                                                                                                                                             >  2:133498/1‑143 (MQ=255)
          ccAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCg                                                                                                                                          >  3:244059/1‑148 (MQ=255)
           cAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTa                                                                                                                                        <  3:16142/149‑1 (MQ=255)
           cAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTa                                                                                                                                        <  4:210800/149‑1 (MQ=255)
            agaagaTGATTCTCTTCTGTAACATCGAGATCGCCAACTACAAGCCGTTCGTCCTGTTCGGCTGCATGAACGTTCTGGAATCCCGTGACATGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAATCTCGGTATCCCGTAc                                                                                                                                       <  6:146440/149‑1 (MQ=255)
            agaagaTCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTAc                                                                                                                                       >  6:97494/1‑149 (MQ=255)
                 aTCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACgtgt                                                                                                                                   >  3:61861/1‑148 (MQ=255)
                  tCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTc                                                                                                                                 <  4:102321/149‑1 (MQ=255)
                    aTTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTc                                                                                                                                 >  1:151801/1‑147 (MQ=255)
                        gcgTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAAcc                                                                                                                                            >  5:76166/1‑132 (MQ=255)
                        gcgTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAAcc                                                                                                                                            <  6:76166/132‑1 (MQ=255)
                              gTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTc                                                                                                                     <  5:97494/149‑1 (MQ=255)
                              gTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTc                                                                                                                     >  7:236863/1‑149 (MQ=255)
                              gTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTc                                                                                                                     >  1:219417/1‑149 (MQ=255)
                                   aTCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTAc                                                                                                                                       <  4:154579/126‑1 (MQ=255)
                                   aTCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTAc                                                                                                                                       >  3:154579/1‑126 (MQ=255)
                                   aTCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGAc                                                                                                                  >  4:198191/1‑147 (MQ=255)
                                       agaTCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGc                                                                                                             >  6:241211/1‑148 (MQ=255)
                                              cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                                     >  6:316931/1‑149 (MQ=255)
                                              cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                                     <  4:75301/149‑1 (MQ=255)
                                              cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                                     <  2:272878/149‑1 (MQ=255)
                                              cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                                     >  1:144639/1‑149 (MQ=255)
                                              cAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGAGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGtt                                                                                                     >  5:242522/1‑149 (MQ=255)
                                               aaCGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTtc                                                                                                    <  3:198191/149‑1 (MQ=255)
                                               aaCGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTtc                                                                                                    <  7:175694/149‑1 (MQ=255)
                                                  gACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTtcgtc                                                                                                 <  8:236863/149‑1 (MQ=255)
                                                    cAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATGCGGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCACGTAAGTGTTCAAGGACAGTGTCGACAAGGCCAACAGTTCGTCgg                                                                                               >  7:139001/1‑149 (MQ=255)
                                                      aGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAgg                                                                                                                             <  7:253826/117‑1 (MQ=255)
                                                      aGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAgg                                                                                                                             >  8:253826/1‑117 (MQ=255)
                                                       gCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTaa                                                                                            >  4:273114/1‑149 (MQ=255)
                                                       gCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAACCCgc                                                                                                                                         >  6:91588/1‑103 (MQ=37)
                                                       gCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTACGGGATACCGAGCTTCTCGGTAACCCgc                                                                                                                                         <  5:91588/104‑2 (MQ=37)
                                                               tCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTAc                                                                                    <  2:158394/149‑1 (MQ=255)
                                                               tCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTAc                                                                                    >  2:152458/1‑149 (MQ=255)
                                                                ccTGTTCGGAGGCATGAACGTTCTGGAATCGCGTGACCTGGCGATGAAGGTATGCGAAGAGTACGTGAGGGTTACAGAGAAGCTCGGTATCCCGTACTTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAAAGTCCTaaa                                                                                   >  7:130024/1‑147 (MQ=255)
                                                                    ttCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTgg                                                                               <  1:152458/149‑1 (MQ=255)
                                                                          ggcATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTTCGAAGAGTACGTGCGGTTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAATGCCAGTTTCGACAAGGCCAACCGTTAGTCGGTAACGTCCTACCGTGGCCCTgg                                                                         <  8:139001/149‑1 (MQ=255)
                                                                           gcATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATACCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTgg                                                                         >  7:85134/1‑148 (MQ=255)
                                                                                      cTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAgg                                                              <  2:219417/148‑1 (MQ=255)
                                                                                       tGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAgggg                                                            >  7:69389/1‑149 (MQ=255)
                                                                                        ggAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGc                                                           >  6:37159/1‑149 (MQ=255)
                                                                                           aTCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTg                                                         >  2:148209/1‑148 (MQ=255)
                                                                                              ccGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTg                                                         >  4:306608/1‑145 (MQ=255)
                                                                                              ccGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTg                                                         >  1:207222/1‑145 (MQ=255)
                                                                                                   aCCTGGCTAAGAAGGTCTGCGAAGAGTAAGTGCGGGTTACCTAGAAGCTCTGTATACCGTACGTTTTCAATGCCATTTTCGACAATGACAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCtt                                                 <  8:130024/148‑1 (MQ=255)
                                                                                                   aCCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCtt                                                 <  5:241211/148‑1 (MQ=255)
                                                                                                   aCCTGGCGATGAAGGTCTGCGAAGAGTACGAGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCtt                                                 <  6:242522/148‑1 (MQ=255)
                                                                                                        gCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGaa                                             <  2:207222/147‑1 (MQ=255)
                                                                                                        gCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGaa                                             <  7:171102/147‑1 (MQ=255)
                                                                                                           aTGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCa                                                                                                                          >  7:103806/1‑67 (MQ=255)
                                                                                                           aTGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCa                                                                                                                          <  8:103806/67‑1 (MQ=255)
                                                                                                           aTGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGaa                                             >  1:293905/1‑144 (MQ=255)
                                                                                                                 gTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAgcg                                  >  2:301062/1‑149 (MQ=255)
                                                                                                                      cGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCt                             >  6:194550/1‑149 (MQ=255)
                                                                                                                       gAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCtt                            >  8:272358/1‑149 (MQ=255)
                                                                                                                           agTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAAc                        <  5:316931/149‑1 (MQ=255)
                                                                                                                              aCGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGt                      <  2:151801/148‑1 (MQ=255)
                                                                                                                                   cGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGt                <  8:85134/149‑1 (MQ=255)
                                                                                                                                   cGGGATACCGAGCTTCTCGGTAACCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGt                >  1:131905/1‑149 (MQ=255)
                                                                                                                                     ggTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGa              <  3:28852/149‑1 (MQ=255)
                                                                                                                                         aCCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGt                >  4:221286/1‑143 (MQ=255)
                                                                                                                                         aCCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGt                <  3:221286/143‑1 (MQ=255)
                                                                                                                                          ccGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGATCAc          <  3:306608/148‑1 (MQ=255)
                                                                                                                                             agaAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCAGCTTCAACGTGCCGGTGATCACCGAt      <  1:148209/149‑1 (MQ=255)
                                                                                                                                                 gCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGATCACCGATGTGc  <  5:194550/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GATCGATGACCCAGAAGATCATTCGCGTCGGTAACATCGAGATCGCCAACGACAAGCCGTTCGTCCTGTTCGGCGGCATGAACGTTCTGGAATCCCGTGACCTGGCGATGAAGGTCTGCGAAGAGTACGTGCGGGTTACCGAGAAGCTCGGTATCCCGTACGTGTTCAAGGCCAGTTTCGACAAGGCCAACCGTTCGTCGGTAACGTCCTACCGTGGCCCTGGCATGGAAGAGGGGCTGAAGATCTTCGAAGAGATCAAGCGCACCTTCAACGTGCCGGTGATCACCGATGTGC  >  NC_002947/1807945‑1808238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑